Antibiotic resistance of bacterial biofilms

Research output: Contribution to journalReviewpeer-review

Standard

Antibiotic resistance of bacterial biofilms. / Høiby, Niels; Bjarnsholt, Thomas; Givskov, Michael; Molin, Søren; Ciofu, Oana.

In: International Journal of Antimicrobial Agents, Vol. 35, No. 4, 2010, p. 322-332.

Research output: Contribution to journalReviewpeer-review

Harvard

Høiby, N, Bjarnsholt, T, Givskov, M, Molin, S & Ciofu, O 2010, 'Antibiotic resistance of bacterial biofilms', International Journal of Antimicrobial Agents, vol. 35, no. 4, pp. 322-332. https://doi.org/10.1016/j.ijantimicag.2009.12.011

APA

Høiby, N., Bjarnsholt, T., Givskov, M., Molin, S., & Ciofu, O. (2010). Antibiotic resistance of bacterial biofilms. International Journal of Antimicrobial Agents, 35(4), 322-332. https://doi.org/10.1016/j.ijantimicag.2009.12.011

Vancouver

Høiby N, Bjarnsholt T, Givskov M, Molin S, Ciofu O. Antibiotic resistance of bacterial biofilms. International Journal of Antimicrobial Agents. 2010;35(4):322-332. https://doi.org/10.1016/j.ijantimicag.2009.12.011

Author

Høiby, Niels ; Bjarnsholt, Thomas ; Givskov, Michael ; Molin, Søren ; Ciofu, Oana. / Antibiotic resistance of bacterial biofilms. In: International Journal of Antimicrobial Agents. 2010 ; Vol. 35, No. 4. pp. 322-332.

Bibtex

@article{972cfdc07d2e11df928f000ea68e967b,
title = "Antibiotic resistance of bacterial biofilms",
abstract = "A biofilm is a structured consortium of bacteria embedded in a self-produced polymer matrix consisting of polysaccharide, protein and DNA. Bacterial biofilms cause chronic infections because they show increased tolerance to antibiotics and disinfectant chemicals as well as resisting phagocytosis and other components of the body's defence system. The persistence of, for example, staphylococcal infections related to foreign bodies is due to biofilm formation. Likewise, chronic Pseudomonas aeruginosa lung infection in cystic fibrosis patients is caused by biofilm-growing mucoid strains. Characteristically, gradients of nutrients and oxygen exist from the top to the bottom of biofilms and these gradients are associated with decreased bacterial metabolic activity and increased doubling times of the bacterial cells; it is these more or less dormant cells that are responsible for some of the tolerance to antibiotics. Biofilm growth is associated with an increased level of mutations as well as with quorum-sensing-regulated mechanisms. Conventional resistance mechanisms such as chromosomal beta-lactamase, upregulated efflux pumps and mutations in antibiotic target molecules in bacteria also contribute to the survival of biofilms. Biofilms can be prevented by early aggressive antibiotic prophylaxis or therapy and they can be treated by chronic suppressive therapy. A promising strategy may be the use of enzymes that can dissolve the biofilm matrix (e.g. DNase and alginate lyase) as well as quorum-sensing inhibitors that increase biofilm susceptibility to antibiotics.",
author = "Niels H{\o}iby and Thomas Bjarnsholt and Michael Givskov and S{\o}ren Molin and Oana Ciofu",
note = "Keywords: Anti-Bacterial Agents; Bacteria; Bacterial Infections; Bacterial Physiological Phenomena; Biofilms; Drug Resistance, Bacterial; Humans",
year = "2010",
doi = "10.1016/j.ijantimicag.2009.12.011",
language = "English",
volume = "35",
pages = "322--332",
journal = "International Journal of Antimicrobial Agents",
issn = "0924-8579",
publisher = "Elsevier",
number = "4",

}

RIS

TY - JOUR

T1 - Antibiotic resistance of bacterial biofilms

AU - Høiby, Niels

AU - Bjarnsholt, Thomas

AU - Givskov, Michael

AU - Molin, Søren

AU - Ciofu, Oana

N1 - Keywords: Anti-Bacterial Agents; Bacteria; Bacterial Infections; Bacterial Physiological Phenomena; Biofilms; Drug Resistance, Bacterial; Humans

PY - 2010

Y1 - 2010

N2 - A biofilm is a structured consortium of bacteria embedded in a self-produced polymer matrix consisting of polysaccharide, protein and DNA. Bacterial biofilms cause chronic infections because they show increased tolerance to antibiotics and disinfectant chemicals as well as resisting phagocytosis and other components of the body's defence system. The persistence of, for example, staphylococcal infections related to foreign bodies is due to biofilm formation. Likewise, chronic Pseudomonas aeruginosa lung infection in cystic fibrosis patients is caused by biofilm-growing mucoid strains. Characteristically, gradients of nutrients and oxygen exist from the top to the bottom of biofilms and these gradients are associated with decreased bacterial metabolic activity and increased doubling times of the bacterial cells; it is these more or less dormant cells that are responsible for some of the tolerance to antibiotics. Biofilm growth is associated with an increased level of mutations as well as with quorum-sensing-regulated mechanisms. Conventional resistance mechanisms such as chromosomal beta-lactamase, upregulated efflux pumps and mutations in antibiotic target molecules in bacteria also contribute to the survival of biofilms. Biofilms can be prevented by early aggressive antibiotic prophylaxis or therapy and they can be treated by chronic suppressive therapy. A promising strategy may be the use of enzymes that can dissolve the biofilm matrix (e.g. DNase and alginate lyase) as well as quorum-sensing inhibitors that increase biofilm susceptibility to antibiotics.

AB - A biofilm is a structured consortium of bacteria embedded in a self-produced polymer matrix consisting of polysaccharide, protein and DNA. Bacterial biofilms cause chronic infections because they show increased tolerance to antibiotics and disinfectant chemicals as well as resisting phagocytosis and other components of the body's defence system. The persistence of, for example, staphylococcal infections related to foreign bodies is due to biofilm formation. Likewise, chronic Pseudomonas aeruginosa lung infection in cystic fibrosis patients is caused by biofilm-growing mucoid strains. Characteristically, gradients of nutrients and oxygen exist from the top to the bottom of biofilms and these gradients are associated with decreased bacterial metabolic activity and increased doubling times of the bacterial cells; it is these more or less dormant cells that are responsible for some of the tolerance to antibiotics. Biofilm growth is associated with an increased level of mutations as well as with quorum-sensing-regulated mechanisms. Conventional resistance mechanisms such as chromosomal beta-lactamase, upregulated efflux pumps and mutations in antibiotic target molecules in bacteria also contribute to the survival of biofilms. Biofilms can be prevented by early aggressive antibiotic prophylaxis or therapy and they can be treated by chronic suppressive therapy. A promising strategy may be the use of enzymes that can dissolve the biofilm matrix (e.g. DNase and alginate lyase) as well as quorum-sensing inhibitors that increase biofilm susceptibility to antibiotics.

U2 - 10.1016/j.ijantimicag.2009.12.011

DO - 10.1016/j.ijantimicag.2009.12.011

M3 - Review

C2 - 20149602

VL - 35

SP - 322

EP - 332

JO - International Journal of Antimicrobial Agents

JF - International Journal of Antimicrobial Agents

SN - 0924-8579

IS - 4

ER -

ID: 20393110