Antibodies against chromosomal beta-lactamase.

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Antibodies against chromosomal beta-lactamase. / Giwercman, B; Rasmussen, J W; Ciofu, Oana; Clemmentsen, I; Schumacher, H; Høiby, N.

In: Antimicrobial Agents and Chemotherapy, Vol. 38, No. 10, 1994, p. 2306-10.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Giwercman, B, Rasmussen, JW, Ciofu, O, Clemmentsen, I, Schumacher, H & Høiby, N 1994, 'Antibodies against chromosomal beta-lactamase.', Antimicrobial Agents and Chemotherapy, vol. 38, no. 10, pp. 2306-10.

APA

Giwercman, B., Rasmussen, J. W., Ciofu, O., Clemmentsen, I., Schumacher, H., & Høiby, N. (1994). Antibodies against chromosomal beta-lactamase. Antimicrobial Agents and Chemotherapy, 38(10), 2306-10.

Vancouver

Giwercman B, Rasmussen JW, Ciofu O, Clemmentsen I, Schumacher H, Høiby N. Antibodies against chromosomal beta-lactamase. Antimicrobial Agents and Chemotherapy. 1994;38(10):2306-10.

Author

Giwercman, B ; Rasmussen, J W ; Ciofu, Oana ; Clemmentsen, I ; Schumacher, H ; Høiby, N. / Antibodies against chromosomal beta-lactamase. In: Antimicrobial Agents and Chemotherapy. 1994 ; Vol. 38, No. 10. pp. 2306-10.

Bibtex

@article{895f9520ba2211ddae57000ea68e967b,
title = "Antibodies against chromosomal beta-lactamase.",
abstract = "A murine monoclonal anti-chromosomal beta-lactamase antibody was developed and an immunoblotting technique was used to study the presence of serum and sputum antibodies against Pseudomonas aeruginosa chromosomal group 1 beta-lactamase in patients with cystic fibrosis (CF). The serum antibody response was studied with serum samples collected in 1992 from 56 CF patients in a cross-sectional study and with serum samples from 18 CF patients in a longitudinal study. Anti-beta-lactamase immunoglobulin G antibodies were present in all of the serum samples from the patients with chronic bronchopulmonary P. aeruginosa infection (CF + P) but in none of the CF patients with no or intermittent P. aeruginosa infection. Anti-beta-lactamase antibodies were present in serum from CF + P patients after six antipseudomonal courses (median) and correlated with infection with a beta-lactam-resistant strain of P. aeruginosa. The sputum antibody response and the beta-lactamase activity in sputum samples from 14 of the CF + P patients were also studied. beta-lactamase antibodies were present in 10 of these samples. P. aeruginosa strains isolated from these samples were partially derepressed, producing group 1 cephalosporinase. We found a wide range of chromosomal beta-lactamase activity in the sputum samples, with no correlation with basal or induced activity of beta-lactamase expression. The presence of anti-beta-lactamase antibodies in endobronchial sputum could be an important factor in the defense against the infection. On the other hand, immune complexes between the beta-lactamase and corresponding antibodies could play a role in the pathogenesis of bronchopulmonary injury in CF by mediating hyperimmune reactions.",
author = "B Giwercman and Rasmussen, {J W} and Oana Ciofu and I Clemmentsen and H Schumacher and N H{\o}iby",
note = "Keywords: Adolescent; Adult; Animals; Antibodies, Bacterial; Child; Chromosomes, Bacterial; Cystic Fibrosis; Humans; Immunoglobulin G; Mice; Mice, Inbred BALB C; Middle Aged; Pseudomonas Infections; Pseudomonas aeruginosa; Sputum; beta-Lactamases",
year = "1994",
language = "English",
volume = "38",
pages = "2306--10",
journal = "Antimicrobial Agents and Chemotherapy",
issn = "0066-4804",
publisher = "American Society for Microbiology",
number = "10",

}

RIS

TY - JOUR

T1 - Antibodies against chromosomal beta-lactamase.

AU - Giwercman, B

AU - Rasmussen, J W

AU - Ciofu, Oana

AU - Clemmentsen, I

AU - Schumacher, H

AU - Høiby, N

N1 - Keywords: Adolescent; Adult; Animals; Antibodies, Bacterial; Child; Chromosomes, Bacterial; Cystic Fibrosis; Humans; Immunoglobulin G; Mice; Mice, Inbred BALB C; Middle Aged; Pseudomonas Infections; Pseudomonas aeruginosa; Sputum; beta-Lactamases

PY - 1994

Y1 - 1994

N2 - A murine monoclonal anti-chromosomal beta-lactamase antibody was developed and an immunoblotting technique was used to study the presence of serum and sputum antibodies against Pseudomonas aeruginosa chromosomal group 1 beta-lactamase in patients with cystic fibrosis (CF). The serum antibody response was studied with serum samples collected in 1992 from 56 CF patients in a cross-sectional study and with serum samples from 18 CF patients in a longitudinal study. Anti-beta-lactamase immunoglobulin G antibodies were present in all of the serum samples from the patients with chronic bronchopulmonary P. aeruginosa infection (CF + P) but in none of the CF patients with no or intermittent P. aeruginosa infection. Anti-beta-lactamase antibodies were present in serum from CF + P patients after six antipseudomonal courses (median) and correlated with infection with a beta-lactam-resistant strain of P. aeruginosa. The sputum antibody response and the beta-lactamase activity in sputum samples from 14 of the CF + P patients were also studied. beta-lactamase antibodies were present in 10 of these samples. P. aeruginosa strains isolated from these samples were partially derepressed, producing group 1 cephalosporinase. We found a wide range of chromosomal beta-lactamase activity in the sputum samples, with no correlation with basal or induced activity of beta-lactamase expression. The presence of anti-beta-lactamase antibodies in endobronchial sputum could be an important factor in the defense against the infection. On the other hand, immune complexes between the beta-lactamase and corresponding antibodies could play a role in the pathogenesis of bronchopulmonary injury in CF by mediating hyperimmune reactions.

AB - A murine monoclonal anti-chromosomal beta-lactamase antibody was developed and an immunoblotting technique was used to study the presence of serum and sputum antibodies against Pseudomonas aeruginosa chromosomal group 1 beta-lactamase in patients with cystic fibrosis (CF). The serum antibody response was studied with serum samples collected in 1992 from 56 CF patients in a cross-sectional study and with serum samples from 18 CF patients in a longitudinal study. Anti-beta-lactamase immunoglobulin G antibodies were present in all of the serum samples from the patients with chronic bronchopulmonary P. aeruginosa infection (CF + P) but in none of the CF patients with no or intermittent P. aeruginosa infection. Anti-beta-lactamase antibodies were present in serum from CF + P patients after six antipseudomonal courses (median) and correlated with infection with a beta-lactam-resistant strain of P. aeruginosa. The sputum antibody response and the beta-lactamase activity in sputum samples from 14 of the CF + P patients were also studied. beta-lactamase antibodies were present in 10 of these samples. P. aeruginosa strains isolated from these samples were partially derepressed, producing group 1 cephalosporinase. We found a wide range of chromosomal beta-lactamase activity in the sputum samples, with no correlation with basal or induced activity of beta-lactamase expression. The presence of anti-beta-lactamase antibodies in endobronchial sputum could be an important factor in the defense against the infection. On the other hand, immune complexes between the beta-lactamase and corresponding antibodies could play a role in the pathogenesis of bronchopulmonary injury in CF by mediating hyperimmune reactions.

M3 - Journal article

C2 - 7840561

VL - 38

SP - 2306

EP - 2310

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

SN - 0066-4804

IS - 10

ER -

ID: 8745184