Anti-Pseudomonas aeruginosa IgY antibodies augment bacterial clearance in a murine pneumonia model
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Anti-Pseudomonas aeruginosa IgY antibodies augment bacterial clearance in a murine pneumonia model. / Thomsen, K.; Christophersen, L.; Bjarnsholt, T.; Jensen, Peter Østrup; Moser, C.; Høiby, N.
In: Journal of Cystic Fibrosis, Vol. 15, No. 2, 03.2016, p. 171-178.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Anti-Pseudomonas aeruginosa IgY antibodies augment bacterial clearance in a murine pneumonia model
AU - Thomsen, K.
AU - Christophersen, L.
AU - Bjarnsholt, T.
AU - Jensen, Peter Østrup
AU - Moser, C.
AU - Høiby, N.
PY - 2016/3
Y1 - 2016/3
N2 - Background: Oral prophylactic therapy by gargling with pathogen-specific egg yolk immunoglobulins (IgY) may reduce the initial airway colonization with Pseudomonas aeruginosa in cystic fibrosis (CF) patients. IgY antibodies impart passive immunization and we investigated the effects of anti-P. aeruginosa IgY antibodies on bacterial eradication in a murine pneumonia model. Methods: P. aeruginosa pneumonia was established in Balb/c mice and the effects of prophylactic IgY administration on lung bacteriology, clinical parameters and subsequent inflammation were compared to controls. Results: Prophylactic administration of IgY antibodies targeting P. aeruginosa significantly reduced the bacterial burden by 2-log 24 h post-infection compared to controls and was accompanied by significantly reduced clinical symptom scores and successive inflammatory cytokine profile indicative of diminished lung inflammation. Conclusions: Passive immunization by anti-P. aeruginosa IgY therapy facilitates promptly bacterial clearance and moderates inflammation in P. aeruginosa lung infection and may serve as an adjunct to antibiotics in reducing early colonization.
AB - Background: Oral prophylactic therapy by gargling with pathogen-specific egg yolk immunoglobulins (IgY) may reduce the initial airway colonization with Pseudomonas aeruginosa in cystic fibrosis (CF) patients. IgY antibodies impart passive immunization and we investigated the effects of anti-P. aeruginosa IgY antibodies on bacterial eradication in a murine pneumonia model. Methods: P. aeruginosa pneumonia was established in Balb/c mice and the effects of prophylactic IgY administration on lung bacteriology, clinical parameters and subsequent inflammation were compared to controls. Results: Prophylactic administration of IgY antibodies targeting P. aeruginosa significantly reduced the bacterial burden by 2-log 24 h post-infection compared to controls and was accompanied by significantly reduced clinical symptom scores and successive inflammatory cytokine profile indicative of diminished lung inflammation. Conclusions: Passive immunization by anti-P. aeruginosa IgY therapy facilitates promptly bacterial clearance and moderates inflammation in P. aeruginosa lung infection and may serve as an adjunct to antibiotics in reducing early colonization.
KW - Cystic fibrosis
KW - IgY
KW - Immunotherapy
KW - Pseudomonas aeruginosa
U2 - 10.1016/j.jcf.2015.08.002
DO - 10.1016/j.jcf.2015.08.002
M3 - Journal article
C2 - 26303991
AN - SCOPUS:84939600789
VL - 15
SP - 171
EP - 178
JO - Journal of Cystic Fibrosis
JF - Journal of Cystic Fibrosis
SN - 1569-1993
IS - 2
ER -
ID: 168882053