Biofilm formation by Pseudomonas aeruginosa wild type, flagella and type IV pili mutants.

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Biofilm formation by Pseudomonas aeruginosa wild type, flagella and type IV pili mutants. / Klausen, Mikkel; Heydorn, Arne; Ragas, Paula; Lambertsen, Lotte; Aaes-Jørgensen, Anders; Molin, Søren; Tolker-Nielsen, Tim.

In: Molecular Microbiology, Vol. 48, No. 6, 2003, p. 1511-24.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Klausen, M, Heydorn, A, Ragas, P, Lambertsen, L, Aaes-Jørgensen, A, Molin, S & Tolker-Nielsen, T 2003, 'Biofilm formation by Pseudomonas aeruginosa wild type, flagella and type IV pili mutants.', Molecular Microbiology, vol. 48, no. 6, pp. 1511-24.

APA

Klausen, M., Heydorn, A., Ragas, P., Lambertsen, L., Aaes-Jørgensen, A., Molin, S., & Tolker-Nielsen, T. (2003). Biofilm formation by Pseudomonas aeruginosa wild type, flagella and type IV pili mutants. Molecular Microbiology, 48(6), 1511-24.

Vancouver

Klausen M, Heydorn A, Ragas P, Lambertsen L, Aaes-Jørgensen A, Molin S et al. Biofilm formation by Pseudomonas aeruginosa wild type, flagella and type IV pili mutants. Molecular Microbiology. 2003;48(6):1511-24.

Author

Klausen, Mikkel ; Heydorn, Arne ; Ragas, Paula ; Lambertsen, Lotte ; Aaes-Jørgensen, Anders ; Molin, Søren ; Tolker-Nielsen, Tim. / Biofilm formation by Pseudomonas aeruginosa wild type, flagella and type IV pili mutants. In: Molecular Microbiology. 2003 ; Vol. 48, No. 6. pp. 1511-24.

Bibtex

@article{bc6d9e00bd4011dd8e02000ea68e967b,
title = "Biofilm formation by Pseudomonas aeruginosa wild type, flagella and type IV pili mutants.",
abstract = "Biofilm formation by Gfp-tagged Pseudomonas aeruginosa PAO1 wild type, flagella and type IV pili mutants in flow chambers irrigated with citrate minimal medium was characterized by the use of confocal laser scanning microscopy and comstat image analysis. Flagella and type IV pili were not necessary for P. aeruginosa initial attachment or biofilm formation, but the cell appendages had roles in biofilm development, as wild type, flagella and type IV pili mutants formed biofilms with different structures. Dynamics and selection during biofilm formation were investigated by tagging the wild type and flagella/type IV mutants with Yfp and Cfp and performing time-lapse confocal laser scanning microscopy in mixed colour biofilms. The initial microcolony formation occurred by clonal growth, after which wild-type P. aeruginosa bacteria spread over the substratum by means of twitching motility. The wild-type biofilms were dynamic compositions with extensive motility, competition and selection occurring during development. Bacterial migration prevented the formation of larger microcolonial structures in the wild-type biofilms. The results are discussed in relation to the current model for P. aeruginosa biofilm development.",
author = "Mikkel Klausen and Arne Heydorn and Paula Ragas and Lotte Lambertsen and Anders Aaes-J{\o}rgensen and S{\o}ren Molin and Tim Tolker-Nielsen",
note = "Keywords: Biofilms; Citrates; Culture Media; Fimbriae, Bacterial; Flagella; Green Fluorescent Proteins; Image Processing, Computer-Assisted; Luminescent Proteins; Microscopy, Confocal; Mutation; Pseudomonas aeruginosa; Software",
year = "2003",
language = "English",
volume = "48",
pages = "1511--24",
journal = "Molecular Microbiology",
issn = "0950-382X",
publisher = "Wiley-Blackwell",
number = "6",

}

RIS

TY - JOUR

T1 - Biofilm formation by Pseudomonas aeruginosa wild type, flagella and type IV pili mutants.

AU - Klausen, Mikkel

AU - Heydorn, Arne

AU - Ragas, Paula

AU - Lambertsen, Lotte

AU - Aaes-Jørgensen, Anders

AU - Molin, Søren

AU - Tolker-Nielsen, Tim

N1 - Keywords: Biofilms; Citrates; Culture Media; Fimbriae, Bacterial; Flagella; Green Fluorescent Proteins; Image Processing, Computer-Assisted; Luminescent Proteins; Microscopy, Confocal; Mutation; Pseudomonas aeruginosa; Software

PY - 2003

Y1 - 2003

N2 - Biofilm formation by Gfp-tagged Pseudomonas aeruginosa PAO1 wild type, flagella and type IV pili mutants in flow chambers irrigated with citrate minimal medium was characterized by the use of confocal laser scanning microscopy and comstat image analysis. Flagella and type IV pili were not necessary for P. aeruginosa initial attachment or biofilm formation, but the cell appendages had roles in biofilm development, as wild type, flagella and type IV pili mutants formed biofilms with different structures. Dynamics and selection during biofilm formation were investigated by tagging the wild type and flagella/type IV mutants with Yfp and Cfp and performing time-lapse confocal laser scanning microscopy in mixed colour biofilms. The initial microcolony formation occurred by clonal growth, after which wild-type P. aeruginosa bacteria spread over the substratum by means of twitching motility. The wild-type biofilms were dynamic compositions with extensive motility, competition and selection occurring during development. Bacterial migration prevented the formation of larger microcolonial structures in the wild-type biofilms. The results are discussed in relation to the current model for P. aeruginosa biofilm development.

AB - Biofilm formation by Gfp-tagged Pseudomonas aeruginosa PAO1 wild type, flagella and type IV pili mutants in flow chambers irrigated with citrate minimal medium was characterized by the use of confocal laser scanning microscopy and comstat image analysis. Flagella and type IV pili were not necessary for P. aeruginosa initial attachment or biofilm formation, but the cell appendages had roles in biofilm development, as wild type, flagella and type IV pili mutants formed biofilms with different structures. Dynamics and selection during biofilm formation were investigated by tagging the wild type and flagella/type IV mutants with Yfp and Cfp and performing time-lapse confocal laser scanning microscopy in mixed colour biofilms. The initial microcolony formation occurred by clonal growth, after which wild-type P. aeruginosa bacteria spread over the substratum by means of twitching motility. The wild-type biofilms were dynamic compositions with extensive motility, competition and selection occurring during development. Bacterial migration prevented the formation of larger microcolonial structures in the wild-type biofilms. The results are discussed in relation to the current model for P. aeruginosa biofilm development.

M3 - Journal article

C2 - 12791135

VL - 48

SP - 1511

EP - 1524

JO - Molecular Microbiology

JF - Molecular Microbiology

SN - 0950-382X

IS - 6

ER -

ID: 8780308