Biofilm formation by Staphylococcus epidermidis on peritoneal dialysis catheters and the effects of extracellular products from Pseudomonas aeruginosa

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Biofilm formation by Staphylococcus epidermidis on peritoneal dialysis catheters and the effects of extracellular products from Pseudomonas aeruginosa. / Pihl, Maria; Arvidsson, Anna; Skepö, Marie; Nilsson, Martin; Givskov, Michael; Tolker-Nielsen, Tim; Svensäter, Gunnel; Davies, Julia R.

In: Pathogens and Disease, Vol. 67, No. 3, 04.2013, p. 192-198.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Pihl, M, Arvidsson, A, Skepö, M, Nilsson, M, Givskov, M, Tolker-Nielsen, T, Svensäter, G & Davies, JR 2013, 'Biofilm formation by Staphylococcus epidermidis on peritoneal dialysis catheters and the effects of extracellular products from Pseudomonas aeruginosa', Pathogens and Disease, vol. 67, no. 3, pp. 192-198. https://doi.org/10.1111/2049-632X.12035

APA

Pihl, M., Arvidsson, A., Skepö, M., Nilsson, M., Givskov, M., Tolker-Nielsen, T., Svensäter, G., & Davies, J. R. (2013). Biofilm formation by Staphylococcus epidermidis on peritoneal dialysis catheters and the effects of extracellular products from Pseudomonas aeruginosa. Pathogens and Disease, 67(3), 192-198. https://doi.org/10.1111/2049-632X.12035

Vancouver

Pihl M, Arvidsson A, Skepö M, Nilsson M, Givskov M, Tolker-Nielsen T et al. Biofilm formation by Staphylococcus epidermidis on peritoneal dialysis catheters and the effects of extracellular products from Pseudomonas aeruginosa. Pathogens and Disease. 2013 Apr;67(3):192-198. https://doi.org/10.1111/2049-632X.12035

Author

Pihl, Maria ; Arvidsson, Anna ; Skepö, Marie ; Nilsson, Martin ; Givskov, Michael ; Tolker-Nielsen, Tim ; Svensäter, Gunnel ; Davies, Julia R. / Biofilm formation by Staphylococcus epidermidis on peritoneal dialysis catheters and the effects of extracellular products from Pseudomonas aeruginosa. In: Pathogens and Disease. 2013 ; Vol. 67, No. 3. pp. 192-198.

Bibtex

@article{c61cdc7430eb4a9abff796e5d8763c2b,
title = "Biofilm formation by Staphylococcus epidermidis on peritoneal dialysis catheters and the effects of extracellular products from Pseudomonas aeruginosa",
abstract = "Biofilm formation by Staphylococcus epidermidis is a cause of infections related to peritoneal dialysis (PD). We have used a PD catheter flow-cell model in combination with confocal scanning laser microscopy and atomic force microscopy to study biofilm formation by S. epidermidis. Adherence to serum-coated catheters was four times greater than to uncoated ones, suggesting that S. epidermidis binds to serum proteins on the catheter surface. Pseudomonas aeruginosa biofilm supernatant interfered with the formation of a serum protein coat thereby reducing the capacity for biofilm formation in S. epidermidis. Supernatants from ΔpelA, ΔpslBCD and ΔrhlAB strains of P. aeruginosa showed no differences from the wild-type supernatant indicating that the effect on serum coat formation was not due to rhamnolipids or the PelA and PslBCD polysaccharides. Supernatant from P. aeruginosa also dispersed established S. epidermidis biofilms. Supernatants lacking PelA or PslBCD showed no differences from the wild type but that from a ΔrhlAB strain, showed reduced, but not abolished, capacity for dispersal. This suggests that rhamnolipids are involved but not wholly responsible for the effect. Thus, supernatants from P. aeruginosa contain promising substances for the prevention and treatment of biofilm infections, although further work is required to identity more active components.",
author = "Maria Pihl and Anna Arvidsson and Marie Skep{\"o} and Martin Nilsson and Michael Givskov and Tim Tolker-Nielsen and Gunnel Svens{\"a}ter and Davies, {Julia R}",
note = "{\textcopyright} 2013 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.",
year = "2013",
month = apr,
doi = "10.1111/2049-632X.12035",
language = "English",
volume = "67",
pages = "192--198",
journal = "FEMS Immunology and Medical Microbiology",
issn = "2049-632X",
publisher = "Oxford University Press",
number = "3",

}

RIS

TY - JOUR

T1 - Biofilm formation by Staphylococcus epidermidis on peritoneal dialysis catheters and the effects of extracellular products from Pseudomonas aeruginosa

AU - Pihl, Maria

AU - Arvidsson, Anna

AU - Skepö, Marie

AU - Nilsson, Martin

AU - Givskov, Michael

AU - Tolker-Nielsen, Tim

AU - Svensäter, Gunnel

AU - Davies, Julia R

N1 - © 2013 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

PY - 2013/4

Y1 - 2013/4

N2 - Biofilm formation by Staphylococcus epidermidis is a cause of infections related to peritoneal dialysis (PD). We have used a PD catheter flow-cell model in combination with confocal scanning laser microscopy and atomic force microscopy to study biofilm formation by S. epidermidis. Adherence to serum-coated catheters was four times greater than to uncoated ones, suggesting that S. epidermidis binds to serum proteins on the catheter surface. Pseudomonas aeruginosa biofilm supernatant interfered with the formation of a serum protein coat thereby reducing the capacity for biofilm formation in S. epidermidis. Supernatants from ΔpelA, ΔpslBCD and ΔrhlAB strains of P. aeruginosa showed no differences from the wild-type supernatant indicating that the effect on serum coat formation was not due to rhamnolipids or the PelA and PslBCD polysaccharides. Supernatant from P. aeruginosa also dispersed established S. epidermidis biofilms. Supernatants lacking PelA or PslBCD showed no differences from the wild type but that from a ΔrhlAB strain, showed reduced, but not abolished, capacity for dispersal. This suggests that rhamnolipids are involved but not wholly responsible for the effect. Thus, supernatants from P. aeruginosa contain promising substances for the prevention and treatment of biofilm infections, although further work is required to identity more active components.

AB - Biofilm formation by Staphylococcus epidermidis is a cause of infections related to peritoneal dialysis (PD). We have used a PD catheter flow-cell model in combination with confocal scanning laser microscopy and atomic force microscopy to study biofilm formation by S. epidermidis. Adherence to serum-coated catheters was four times greater than to uncoated ones, suggesting that S. epidermidis binds to serum proteins on the catheter surface. Pseudomonas aeruginosa biofilm supernatant interfered with the formation of a serum protein coat thereby reducing the capacity for biofilm formation in S. epidermidis. Supernatants from ΔpelA, ΔpslBCD and ΔrhlAB strains of P. aeruginosa showed no differences from the wild-type supernatant indicating that the effect on serum coat formation was not due to rhamnolipids or the PelA and PslBCD polysaccharides. Supernatant from P. aeruginosa also dispersed established S. epidermidis biofilms. Supernatants lacking PelA or PslBCD showed no differences from the wild type but that from a ΔrhlAB strain, showed reduced, but not abolished, capacity for dispersal. This suggests that rhamnolipids are involved but not wholly responsible for the effect. Thus, supernatants from P. aeruginosa contain promising substances for the prevention and treatment of biofilm infections, although further work is required to identity more active components.

U2 - 10.1111/2049-632X.12035

DO - 10.1111/2049-632X.12035

M3 - Journal article

C2 - 23620182

VL - 67

SP - 192

EP - 198

JO - FEMS Immunology and Medical Microbiology

JF - FEMS Immunology and Medical Microbiology

SN - 2049-632X

IS - 3

ER -

ID: 45954695