Biofilms of pathogenic nontuberculous mycobacteria targeted by new therapeutic approaches

Research output: Contribution to journal › Journal article › Research › peer-review

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Biofilms of pathogenic nontuberculous mycobacteria targeted by new therapeutic approaches. / Aung, Thet Tun; Yam, Joey Kuok Hoong; Lin, Shuimu; Salleh, Shuhaida Mohamed; Givskov, Michael; Liu, Shouping; Lwin, Nyein Chan; Yang, Liang; Beuerman, Roger W.

In: Antimicrobial Agents and Chemotherapy, Vol. 60, No. 1, 01.01.2016, p. 24-35.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Aung, TT, Yam, JKH, Lin, S, Salleh, SM, Givskov, M, Liu, S, Lwin, NC, Yang, L & Beuerman, RW 2016, 'Biofilms of pathogenic nontuberculous mycobacteria targeted by new therapeutic approaches', Antimicrobial Agents and Chemotherapy, vol. 60, no. 1, pp. 24-35. https://doi.org/10.1128/AAC.01509-15

APA

Aung, T. T., Yam, J. K. H., Lin, S., Salleh, S. M., Givskov, M., Liu, S., Lwin, N. C., Yang, L., & Beuerman, R. W. (2016). Biofilms of pathogenic nontuberculous mycobacteria targeted by new therapeutic approaches. Antimicrobial Agents and Chemotherapy, 60(1), 24-35. https://doi.org/10.1128/AAC.01509-15

Vancouver

Aung TT, Yam JKH, Lin S, Salleh SM, Givskov M, Liu S et al. Biofilms of pathogenic nontuberculous mycobacteria targeted by new therapeutic approaches. Antimicrobial Agents and Chemotherapy. 2016 Jan 1;60(1):24-35. https://doi.org/10.1128/AAC.01509-15

Author

Aung, Thet Tun ; Yam, Joey Kuok Hoong ; Lin, Shuimu ; Salleh, Shuhaida Mohamed ; Givskov, Michael ; Liu, Shouping ; Lwin, Nyein Chan ; Yang, Liang ; Beuerman, Roger W. / Biofilms of pathogenic nontuberculous mycobacteria targeted by new therapeutic approaches. In: Antimicrobial Agents and Chemotherapy. 2016 ; Vol. 60, No. 1. pp. 24-35.

Bibtex

@article{40c3c9f23884436ca4f0d2d9609e6624,
title = "Biofilms of pathogenic nontuberculous mycobacteria targeted by new therapeutic approaches",
abstract = "Microbial infections of the cornea are potentially devastating and can result in permanent visual loss or require vision-rescuing surgery. In recent years, there has been an increasing number of reports on nontuberculous mycobacterial infections of the cornea. Challenges to the management of nontuberculous mycobacterial keratitis include delayed laboratory detection, low index of clinical suspicion, poor drug penetration, slow response to therapy, and prolonged use of antibiotic combinations. The ability of nontuberculous mycobacteria to evade the host immune response and the ability to adhere and to form biofilms on biological and synthetic substrates contribute to the issue. Therefore, there is an urgent need for new antimicrobial compounds that can overcome these problems. In this study, we evaluated the biofilm architectures for Mycobacterium chelonae and Mycobacterium fortuitum in dynamic flow cell chamber and 8-well chamber slide models. Our results showed that mycobacterial biofilms were quite resistant to conventional antibiotics. However, DNase treatment could be used to overcome biofilm resistance. Moreover, we successfully evaluated a new antimicrobial compound (AM-228) that was effective not only for planktonic mycobacterial cells but also for biofilm treatment and was compared favorably with the most successful {"}fourth-generation{"} fluoroquinolone, gatifloxacin. Finally, a new treatment strategy emerged: a combination of DNase with an antibiotic was more effective than an antibiotic alone.",
author = "Aung, {Thet Tun} and Yam, {Joey Kuok Hoong} and Shuimu Lin and Salleh, {Shuhaida Mohamed} and Michael Givskov and Shouping Liu and Lwin, {Nyein Chan} and Liang Yang and Beuerman, {Roger W.}",
note = "Publisher Copyright: Copyright {\textcopyright} 2015 Aung et al.",
year = "2016",
month = jan,
day = "1",
doi = "10.1128/AAC.01509-15",
language = "English",
volume = "60",
pages = "24--35",
journal = "Antimicrobial Agents and Chemotherapy",
issn = "0066-4804",
publisher = "American Society for Microbiology",
number = "1",

}

RIS

TY - JOUR

T1 - Biofilms of pathogenic nontuberculous mycobacteria targeted by new therapeutic approaches

AU - Aung, Thet Tun

AU - Yam, Joey Kuok Hoong

AU - Lin, Shuimu

AU - Salleh, Shuhaida Mohamed

AU - Givskov, Michael

AU - Liu, Shouping

AU - Lwin, Nyein Chan

AU - Yang, Liang

AU - Beuerman, Roger W.

N1 - Publisher Copyright: Copyright © 2015 Aung et al.

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Microbial infections of the cornea are potentially devastating and can result in permanent visual loss or require vision-rescuing surgery. In recent years, there has been an increasing number of reports on nontuberculous mycobacterial infections of the cornea. Challenges to the management of nontuberculous mycobacterial keratitis include delayed laboratory detection, low index of clinical suspicion, poor drug penetration, slow response to therapy, and prolonged use of antibiotic combinations. The ability of nontuberculous mycobacteria to evade the host immune response and the ability to adhere and to form biofilms on biological and synthetic substrates contribute to the issue. Therefore, there is an urgent need for new antimicrobial compounds that can overcome these problems. In this study, we evaluated the biofilm architectures for Mycobacterium chelonae and Mycobacterium fortuitum in dynamic flow cell chamber and 8-well chamber slide models. Our results showed that mycobacterial biofilms were quite resistant to conventional antibiotics. However, DNase treatment could be used to overcome biofilm resistance. Moreover, we successfully evaluated a new antimicrobial compound (AM-228) that was effective not only for planktonic mycobacterial cells but also for biofilm treatment and was compared favorably with the most successful "fourth-generation" fluoroquinolone, gatifloxacin. Finally, a new treatment strategy emerged: a combination of DNase with an antibiotic was more effective than an antibiotic alone.

AB - Microbial infections of the cornea are potentially devastating and can result in permanent visual loss or require vision-rescuing surgery. In recent years, there has been an increasing number of reports on nontuberculous mycobacterial infections of the cornea. Challenges to the management of nontuberculous mycobacterial keratitis include delayed laboratory detection, low index of clinical suspicion, poor drug penetration, slow response to therapy, and prolonged use of antibiotic combinations. The ability of nontuberculous mycobacteria to evade the host immune response and the ability to adhere and to form biofilms on biological and synthetic substrates contribute to the issue. Therefore, there is an urgent need for new antimicrobial compounds that can overcome these problems. In this study, we evaluated the biofilm architectures for Mycobacterium chelonae and Mycobacterium fortuitum in dynamic flow cell chamber and 8-well chamber slide models. Our results showed that mycobacterial biofilms were quite resistant to conventional antibiotics. However, DNase treatment could be used to overcome biofilm resistance. Moreover, we successfully evaluated a new antimicrobial compound (AM-228) that was effective not only for planktonic mycobacterial cells but also for biofilm treatment and was compared favorably with the most successful "fourth-generation" fluoroquinolone, gatifloxacin. Finally, a new treatment strategy emerged: a combination of DNase with an antibiotic was more effective than an antibiotic alone.

UR - http://www.scopus.com/inward/record.url?scp=84957928103&partnerID=8YFLogxK

U2 - 10.1128/AAC.01509-15

DO - 10.1128/AAC.01509-15

M3 - Journal article

C2 - 26459903

AN - SCOPUS:84957928103

VL - 60

SP - 24

EP - 35

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

SN - 0066-4804

IS - 1

ER -

ID: 340025806