Bursting the bubble on bacterial biofilms: a flow cell methodology
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Bursting the bubble on bacterial biofilms : a flow cell methodology. / Crusz, Shanika A; Popat, Roman; Rybtke, Morten Theil; Cámara, Miguel; Givskov, Michael; Tolker-Nielsen, Tim; Diggle, Stephen P; Williams, Paul.
In: Biofouling, Vol. 28, No. 8, 2012, p. 835-842.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Bursting the bubble on bacterial biofilms
T2 - a flow cell methodology
AU - Crusz, Shanika A
AU - Popat, Roman
AU - Rybtke, Morten Theil
AU - Cámara, Miguel
AU - Givskov, Michael
AU - Tolker-Nielsen, Tim
AU - Diggle, Stephen P
AU - Williams, Paul
PY - 2012
Y1 - 2012
N2 - The flow cell biofilm system is an important and widely used tool for the in vitro cultivation and evaluation of bacterial biofilms under hydrodynamic conditions of flow. This paper provides an introduction to the background and use of such systems, accompanied by a detailed guide to the assembly of the apparatus including the description of new modifications which enhance its performance. As such, this is an essential guide for the novice biofilm researcher as well as providing valuable trouble-shooting techniques for even the most experienced laboratories. The adoption of a common and reliable methodology amongst researchers would enable findings to be shared and replicated amongst the biofilm research community, with the overall aim of advancing understanding and management of these complex and widespread bacterial communities.
AB - The flow cell biofilm system is an important and widely used tool for the in vitro cultivation and evaluation of bacterial biofilms under hydrodynamic conditions of flow. This paper provides an introduction to the background and use of such systems, accompanied by a detailed guide to the assembly of the apparatus including the description of new modifications which enhance its performance. As such, this is an essential guide for the novice biofilm researcher as well as providing valuable trouble-shooting techniques for even the most experienced laboratories. The adoption of a common and reliable methodology amongst researchers would enable findings to be shared and replicated amongst the biofilm research community, with the overall aim of advancing understanding and management of these complex and widespread bacterial communities.
U2 - 10.1080/08927014.2012.716044
DO - 10.1080/08927014.2012.716044
M3 - Journal article
C2 - 22877233
VL - 28
SP - 835
EP - 842
JO - Biofouling
JF - Biofouling
SN - 0892-7014
IS - 8
ER -
ID: 40262917