C-di-GMP regulates antimicrobial peptide resistance in Pseudomonas aeruginosa

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

C-di-GMP regulates antimicrobial peptide resistance in Pseudomonas aeruginosa. / Chua, Song Lin; Tan, Sean Yang-Yi; Rybtke, Morten Theil; Chen, Yicai; Rice, Scott A; Kjelleberg, Staffan; Tolker-Nielsen, Tim; Yang, Liang; Givskov, Michael.

In: Antimicrobial Agents and Chemotherapy, Vol. 57, No. 5, 2013, p. 2066-2075.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Chua, SL, Tan, SY-Y, Rybtke, MT, Chen, Y, Rice, SA, Kjelleberg, S, Tolker-Nielsen, T, Yang, L & Givskov, M 2013, 'C-di-GMP regulates antimicrobial peptide resistance in Pseudomonas aeruginosa', Antimicrobial Agents and Chemotherapy, vol. 57, no. 5, pp. 2066-2075. https://doi.org/10.1128/AAC.02499-12

APA

Chua, S. L., Tan, S. Y-Y., Rybtke, M. T., Chen, Y., Rice, S. A., Kjelleberg, S., Tolker-Nielsen, T., Yang, L., & Givskov, M. (2013). C-di-GMP regulates antimicrobial peptide resistance in Pseudomonas aeruginosa. Antimicrobial Agents and Chemotherapy, 57(5), 2066-2075. https://doi.org/10.1128/AAC.02499-12

Vancouver

Chua SL, Tan SY-Y, Rybtke MT, Chen Y, Rice SA, Kjelleberg S et al. C-di-GMP regulates antimicrobial peptide resistance in Pseudomonas aeruginosa. Antimicrobial Agents and Chemotherapy. 2013;57(5):2066-2075. https://doi.org/10.1128/AAC.02499-12

Author

Chua, Song Lin ; Tan, Sean Yang-Yi ; Rybtke, Morten Theil ; Chen, Yicai ; Rice, Scott A ; Kjelleberg, Staffan ; Tolker-Nielsen, Tim ; Yang, Liang ; Givskov, Michael. / C-di-GMP regulates antimicrobial peptide resistance in Pseudomonas aeruginosa. In: Antimicrobial Agents and Chemotherapy. 2013 ; Vol. 57, No. 5. pp. 2066-2075.

Bibtex

@article{8206e70fac334fc9b63719f8a1f2d33a,
title = "C-di-GMP regulates antimicrobial peptide resistance in Pseudomonas aeruginosa",
abstract = "Bis-(3'-5')-cyclic dimeric GMP (c-di-GMP) is an intracellular second messenger which controls the life styles of many bacteria. A high intracellular level of c-di-GMP induces a biofilm lifestyle, whereas a low intracellular level of c-di-GMP stimulates dispersal of biofilms and promotes a planktonic lifestyle. Here, we used expression of different reporters to show that planktonic cells (PCells), biofilm cells (BCells) and cells dispersed from biofilms (DCells) had distinct intracellular c-di-GMP levels. Proteomics analysis showed that the low intracellular c-di-GMP level of DCells induced the expression of proteins required for the virulence and development of antimicrobial peptide resistance in P. aeruginosa. In accordance, P. aeruginosa cells with low c-di-GMP levels were found to be more resistant to colistin than P. aeruginosa cells with high c-di-GMP levels. This contradicts the current dogma stating that dispersed cells are inevitably more susceptible to antibiotics than their sessile counterparts.",
author = "Chua, {Song Lin} and Tan, {Sean Yang-Yi} and Rybtke, {Morten Theil} and Yicai Chen and Rice, {Scott A} and Staffan Kjelleberg and Tim Tolker-Nielsen and Liang Yang and Michael Givskov",
year = "2013",
doi = "10.1128/AAC.02499-12",
language = "English",
volume = "57",
pages = "2066--2075",
journal = "Antimicrobial Agents and Chemotherapy",
issn = "0066-4804",
publisher = "American Society for Microbiology",
number = "5",

}

RIS

TY - JOUR

T1 - C-di-GMP regulates antimicrobial peptide resistance in Pseudomonas aeruginosa

AU - Chua, Song Lin

AU - Tan, Sean Yang-Yi

AU - Rybtke, Morten Theil

AU - Chen, Yicai

AU - Rice, Scott A

AU - Kjelleberg, Staffan

AU - Tolker-Nielsen, Tim

AU - Yang, Liang

AU - Givskov, Michael

PY - 2013

Y1 - 2013

N2 - Bis-(3'-5')-cyclic dimeric GMP (c-di-GMP) is an intracellular second messenger which controls the life styles of many bacteria. A high intracellular level of c-di-GMP induces a biofilm lifestyle, whereas a low intracellular level of c-di-GMP stimulates dispersal of biofilms and promotes a planktonic lifestyle. Here, we used expression of different reporters to show that planktonic cells (PCells), biofilm cells (BCells) and cells dispersed from biofilms (DCells) had distinct intracellular c-di-GMP levels. Proteomics analysis showed that the low intracellular c-di-GMP level of DCells induced the expression of proteins required for the virulence and development of antimicrobial peptide resistance in P. aeruginosa. In accordance, P. aeruginosa cells with low c-di-GMP levels were found to be more resistant to colistin than P. aeruginosa cells with high c-di-GMP levels. This contradicts the current dogma stating that dispersed cells are inevitably more susceptible to antibiotics than their sessile counterparts.

AB - Bis-(3'-5')-cyclic dimeric GMP (c-di-GMP) is an intracellular second messenger which controls the life styles of many bacteria. A high intracellular level of c-di-GMP induces a biofilm lifestyle, whereas a low intracellular level of c-di-GMP stimulates dispersal of biofilms and promotes a planktonic lifestyle. Here, we used expression of different reporters to show that planktonic cells (PCells), biofilm cells (BCells) and cells dispersed from biofilms (DCells) had distinct intracellular c-di-GMP levels. Proteomics analysis showed that the low intracellular c-di-GMP level of DCells induced the expression of proteins required for the virulence and development of antimicrobial peptide resistance in P. aeruginosa. In accordance, P. aeruginosa cells with low c-di-GMP levels were found to be more resistant to colistin than P. aeruginosa cells with high c-di-GMP levels. This contradicts the current dogma stating that dispersed cells are inevitably more susceptible to antibiotics than their sessile counterparts.

U2 - 10.1128/AAC.02499-12

DO - 10.1128/AAC.02499-12

M3 - Journal article

C2 - 23403434

VL - 57

SP - 2066

EP - 2075

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

SN - 0066-4804

IS - 5

ER -

ID: 44310769