Cloning and characterization of two plasmids from Bacillus thuringiensis in Bacillus subtilis.

Research output: Contribution to journal › Journal article › Research › peer-review

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Cloning and characterization of two plasmids from Bacillus thuringiensis in Bacillus subtilis. / Boe, L; Tolker-Nielsen, Tim; Madsen, S M; Andrup, L; Bolander, G.

In: Plasmid, Vol. 25, No. 3, 1991, p. 190-7.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Boe, L, Tolker-Nielsen, T, Madsen, SM, Andrup, L & Bolander, G 1991, 'Cloning and characterization of two plasmids from Bacillus thuringiensis in Bacillus subtilis.', Plasmid, vol. 25, no. 3, pp. 190-7.

APA

Boe, L., Tolker-Nielsen, T., Madsen, S. M., Andrup, L., & Bolander, G. (1991). Cloning and characterization of two plasmids from Bacillus thuringiensis in Bacillus subtilis. Plasmid, 25(3), 190-7.

Vancouver

Boe L, Tolker-Nielsen T, Madsen SM, Andrup L, Bolander G. Cloning and characterization of two plasmids from Bacillus thuringiensis in Bacillus subtilis. Plasmid. 1991;25(3):190-7.

Author

Boe, L ; Tolker-Nielsen, Tim ; Madsen, S M ; Andrup, L ; Bolander, G. / Cloning and characterization of two plasmids from Bacillus thuringiensis in Bacillus subtilis. In: Plasmid. 1991 ; Vol. 25, No. 3. pp. 190-7.

Bibtex

@article{1289faa0bcc311dd8e02000ea68e967b,
title = "Cloning and characterization of two plasmids from Bacillus thuringiensis in Bacillus subtilis.",
abstract = "Bacillus thuringiensis subspecies israliensis plasmids pTX14-1 and pTX14-3 were cloned and analyzed by Southern blot hybridization for their replication mechanism in Bacillus subtilis. The cloning of pTX14-1 into the replicon deficient vector pBOE335 showed the usual characteristics of single-stranded DNA plasmids, i.e., it generated circular single-stranded DNA and high molecular weight (HMW) multimers. The other plasmid, pTX14-3, behaved differently; it generated neither single-stranded DNA nor HMW multimers. Treatment with rifampicin did not result in the accumulation of single-stranded DNA. However, deletion of an EcoRI-PstI fragment resulted in the accumulation of both single-stranded DNA and HMW multimers. From various deletion derivatives, we have mapped the minus origin and the locus responsible for suppression of HMW multimer formation. Full activity of the minus origin and of the locus suppressing HMW formation was only observed on the native replicon, indicating a coupling to the plus strand synthesis.",
author = "L Boe and Tim Tolker-Nielsen and Madsen, {S M} and L Andrup and G Bolander",
note = "Keywords: Bacillus subtilis; Bacillus thuringiensis; Blotting, Southern; Chromosome Deletion; Cloning, Molecular; DNA, Bacterial; Plasmids; Replicon; Restriction Mapping",
year = "1991",
language = "English",
volume = "25",
pages = "190--7",
journal = "Plasmid",
issn = "0147-619X",
publisher = "Academic Press",
number = "3",

}

RIS

TY - JOUR

T1 - Cloning and characterization of two plasmids from Bacillus thuringiensis in Bacillus subtilis.

AU - Boe, L

AU - Tolker-Nielsen, Tim

AU - Madsen, S M

AU - Andrup, L

AU - Bolander, G

N1 - Keywords: Bacillus subtilis; Bacillus thuringiensis; Blotting, Southern; Chromosome Deletion; Cloning, Molecular; DNA, Bacterial; Plasmids; Replicon; Restriction Mapping

PY - 1991

Y1 - 1991

N2 - Bacillus thuringiensis subspecies israliensis plasmids pTX14-1 and pTX14-3 were cloned and analyzed by Southern blot hybridization for their replication mechanism in Bacillus subtilis. The cloning of pTX14-1 into the replicon deficient vector pBOE335 showed the usual characteristics of single-stranded DNA plasmids, i.e., it generated circular single-stranded DNA and high molecular weight (HMW) multimers. The other plasmid, pTX14-3, behaved differently; it generated neither single-stranded DNA nor HMW multimers. Treatment with rifampicin did not result in the accumulation of single-stranded DNA. However, deletion of an EcoRI-PstI fragment resulted in the accumulation of both single-stranded DNA and HMW multimers. From various deletion derivatives, we have mapped the minus origin and the locus responsible for suppression of HMW multimer formation. Full activity of the minus origin and of the locus suppressing HMW formation was only observed on the native replicon, indicating a coupling to the plus strand synthesis.

AB - Bacillus thuringiensis subspecies israliensis plasmids pTX14-1 and pTX14-3 were cloned and analyzed by Southern blot hybridization for their replication mechanism in Bacillus subtilis. The cloning of pTX14-1 into the replicon deficient vector pBOE335 showed the usual characteristics of single-stranded DNA plasmids, i.e., it generated circular single-stranded DNA and high molecular weight (HMW) multimers. The other plasmid, pTX14-3, behaved differently; it generated neither single-stranded DNA nor HMW multimers. Treatment with rifampicin did not result in the accumulation of single-stranded DNA. However, deletion of an EcoRI-PstI fragment resulted in the accumulation of both single-stranded DNA and HMW multimers. From various deletion derivatives, we have mapped the minus origin and the locus responsible for suppression of HMW multimer formation. Full activity of the minus origin and of the locus suppressing HMW formation was only observed on the native replicon, indicating a coupling to the plus strand synthesis.

M3 - Journal article

C2 - 1924556

VL - 25

SP - 190

EP - 197

JO - Plasmid

JF - Plasmid

SN - 0147-619X

IS - 3

ER -

ID: 8778913