Colistin-tobramycin combinations are superior to monotherapy concerning the killing of biofilm Pseudomonas aeruginosa

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Colistin-tobramycin combinations are superior to monotherapy concerning the killing of biofilm Pseudomonas aeruginosa. / Herrmann, Gloria; Yang, Liang; Wu, Hong; Song, Zhijun; Wang, Hengzhuang; Høiby, Niels; Ulrich, Martina; Molin, Søren; Riethmüller, Joachim; Döring, Gerd.

In: The Journal of Infectious Diseases, Vol. 202, No. 10, 15.11.2010, p. 1585-92.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Herrmann, G, Yang, L, Wu, H, Song, Z, Wang, H, Høiby, N, Ulrich, M, Molin, S, Riethmüller, J & Döring, G 2010, 'Colistin-tobramycin combinations are superior to monotherapy concerning the killing of biofilm Pseudomonas aeruginosa', The Journal of Infectious Diseases, vol. 202, no. 10, pp. 1585-92. https://doi.org/10.1086/656788

APA

Herrmann, G., Yang, L., Wu, H., Song, Z., Wang, H., Høiby, N., Ulrich, M., Molin, S., Riethmüller, J., & Döring, G. (2010). Colistin-tobramycin combinations are superior to monotherapy concerning the killing of biofilm Pseudomonas aeruginosa. The Journal of Infectious Diseases, 202(10), 1585-92. https://doi.org/10.1086/656788

Vancouver

Herrmann G, Yang L, Wu H, Song Z, Wang H, Høiby N et al. Colistin-tobramycin combinations are superior to monotherapy concerning the killing of biofilm Pseudomonas aeruginosa. The Journal of Infectious Diseases. 2010 Nov 15;202(10):1585-92. https://doi.org/10.1086/656788

Author

Herrmann, Gloria ; Yang, Liang ; Wu, Hong ; Song, Zhijun ; Wang, Hengzhuang ; Høiby, Niels ; Ulrich, Martina ; Molin, Søren ; Riethmüller, Joachim ; Döring, Gerd. / Colistin-tobramycin combinations are superior to monotherapy concerning the killing of biofilm Pseudomonas aeruginosa. In: The Journal of Infectious Diseases. 2010 ; Vol. 202, No. 10. pp. 1585-92.

Bibtex

@article{a304c06a2dfe46fa9e36d7d3d48a86ca,
title = "Colistin-tobramycin combinations are superior to monotherapy concerning the killing of biofilm Pseudomonas aeruginosa",
abstract = "BACKGROUND: Antibiotic combination therapy might be more efficient than single antibiotics to combat Pseudomonas aeruginosa biofilms in the airways of patients with cystic fibrosis. We tested the ability of colistin sulphate-tobramycin combinations and single antibiotics to kill P. aeruginosa biofilms.METHODS: P. aeruginosa biofilms were generated in vitro and in rat lungs. In a pilot study, 5 patients with cystic fibrosis inhaled colistin and then tobramycin for 4 weeks. The changes in P. aeruginosa counts and lung function were assessed before and after therapy.RESULTS: Antibiotic combination therapy significantly reduced the number of P. aeruginosa cells in P. aeruginosa biofilm models in vitro. When rats were challenged with 1 x 10(7) cfu of P. aeruginosa, which was embedded in alginate beads, mortality rates, lung pathologic findings, and bacterial colony-forming unit counts were significantly lower after 7 days in animals receiving antibiotic combination than in animals receiving single antibiotics. In patients with cystic fibrosis, inhaled colistin-tobramycin was well tolerated and resulted in a mean decrease of 2.52 + /- 2.5 log(10) cfu of P. aeruginosa per milliliter of sputum (P = .027). Measurements of forced expiratory volume in 1 s, obtained both before and after the study, did not differ significantly.CONCLUSION: Colistin-tobramycin combinations are more efficient than respective single antibiotics for killing P. aeruginosa in biofilms in vitro, and they significantly reduced P. aeruginosa cell counts in a rat lung infection model and in patients with cystic fibrosis.",
keywords = "Administration, Inhalation, Adult, Animals, Anti-Bacterial Agents, Biofilms, Colistin, Cystic Fibrosis, Drug Therapy, Combination, Female, Humans, Lung, Male, Microbial Sensitivity Tests, Pilot Projects, Pseudomonas Infections, Pseudomonas aeruginosa, Rats, Rats, Inbred Lew, Tobramycin, Treatment Outcome",
author = "Gloria Herrmann and Liang Yang and Hong Wu and Zhijun Song and Hengzhuang Wang and Niels H{\o}iby and Martina Ulrich and S{\o}ren Molin and Joachim Riethm{\"u}ller and Gerd D{\"o}ring",
year = "2010",
month = nov,
day = "15",
doi = "10.1086/656788",
language = "English",
volume = "202",
pages = "1585--92",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "10",

}

RIS

TY - JOUR

T1 - Colistin-tobramycin combinations are superior to monotherapy concerning the killing of biofilm Pseudomonas aeruginosa

AU - Herrmann, Gloria

AU - Yang, Liang

AU - Wu, Hong

AU - Song, Zhijun

AU - Wang, Hengzhuang

AU - Høiby, Niels

AU - Ulrich, Martina

AU - Molin, Søren

AU - Riethmüller, Joachim

AU - Döring, Gerd

PY - 2010/11/15

Y1 - 2010/11/15

N2 - BACKGROUND: Antibiotic combination therapy might be more efficient than single antibiotics to combat Pseudomonas aeruginosa biofilms in the airways of patients with cystic fibrosis. We tested the ability of colistin sulphate-tobramycin combinations and single antibiotics to kill P. aeruginosa biofilms.METHODS: P. aeruginosa biofilms were generated in vitro and in rat lungs. In a pilot study, 5 patients with cystic fibrosis inhaled colistin and then tobramycin for 4 weeks. The changes in P. aeruginosa counts and lung function were assessed before and after therapy.RESULTS: Antibiotic combination therapy significantly reduced the number of P. aeruginosa cells in P. aeruginosa biofilm models in vitro. When rats were challenged with 1 x 10(7) cfu of P. aeruginosa, which was embedded in alginate beads, mortality rates, lung pathologic findings, and bacterial colony-forming unit counts were significantly lower after 7 days in animals receiving antibiotic combination than in animals receiving single antibiotics. In patients with cystic fibrosis, inhaled colistin-tobramycin was well tolerated and resulted in a mean decrease of 2.52 + /- 2.5 log(10) cfu of P. aeruginosa per milliliter of sputum (P = .027). Measurements of forced expiratory volume in 1 s, obtained both before and after the study, did not differ significantly.CONCLUSION: Colistin-tobramycin combinations are more efficient than respective single antibiotics for killing P. aeruginosa in biofilms in vitro, and they significantly reduced P. aeruginosa cell counts in a rat lung infection model and in patients with cystic fibrosis.

AB - BACKGROUND: Antibiotic combination therapy might be more efficient than single antibiotics to combat Pseudomonas aeruginosa biofilms in the airways of patients with cystic fibrosis. We tested the ability of colistin sulphate-tobramycin combinations and single antibiotics to kill P. aeruginosa biofilms.METHODS: P. aeruginosa biofilms were generated in vitro and in rat lungs. In a pilot study, 5 patients with cystic fibrosis inhaled colistin and then tobramycin for 4 weeks. The changes in P. aeruginosa counts and lung function were assessed before and after therapy.RESULTS: Antibiotic combination therapy significantly reduced the number of P. aeruginosa cells in P. aeruginosa biofilm models in vitro. When rats were challenged with 1 x 10(7) cfu of P. aeruginosa, which was embedded in alginate beads, mortality rates, lung pathologic findings, and bacterial colony-forming unit counts were significantly lower after 7 days in animals receiving antibiotic combination than in animals receiving single antibiotics. In patients with cystic fibrosis, inhaled colistin-tobramycin was well tolerated and resulted in a mean decrease of 2.52 + /- 2.5 log(10) cfu of P. aeruginosa per milliliter of sputum (P = .027). Measurements of forced expiratory volume in 1 s, obtained both before and after the study, did not differ significantly.CONCLUSION: Colistin-tobramycin combinations are more efficient than respective single antibiotics for killing P. aeruginosa in biofilms in vitro, and they significantly reduced P. aeruginosa cell counts in a rat lung infection model and in patients with cystic fibrosis.

KW - Administration, Inhalation

KW - Adult

KW - Animals

KW - Anti-Bacterial Agents

KW - Biofilms

KW - Colistin

KW - Cystic Fibrosis

KW - Drug Therapy, Combination

KW - Female

KW - Humans

KW - Lung

KW - Male

KW - Microbial Sensitivity Tests

KW - Pilot Projects

KW - Pseudomonas Infections

KW - Pseudomonas aeruginosa

KW - Rats

KW - Rats, Inbred Lew

KW - Tobramycin

KW - Treatment Outcome

U2 - 10.1086/656788

DO - 10.1086/656788

M3 - Journal article

C2 - 20942647

VL - 202

SP - 1585

EP - 1592

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

IS - 10

ER -

ID: 113179749