Colony morphology and transcriptome profiling of Pseudomonas putida KT2440 and its mutants deficient in alginate or all EPS synthesis under controlled matric potentials

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Colony morphology and transcriptome profiling of Pseudomonas putida KT2440 and its mutants deficient in alginate or all EPS synthesis under controlled matric potentials. / Gulez, Gamze; Altintas, Ali; Fazli, Mustafa; Dechesne, Arnaud; Workman, Christopher; Tolker-Nielsen, Tim; Smets, Barth F.

In: MicrobiologyOpen, Vol. 3, No. 4, 2014, p. 457-469.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Gulez, G, Altintas, A, Fazli, M, Dechesne, A, Workman, C, Tolker-Nielsen, T & Smets, BF 2014, 'Colony morphology and transcriptome profiling of Pseudomonas putida KT2440 and its mutants deficient in alginate or all EPS synthesis under controlled matric potentials', MicrobiologyOpen, vol. 3, no. 4, pp. 457-469. https://doi.org/10.1002/mbo3.180

APA

Gulez, G., Altintas, A., Fazli, M., Dechesne, A., Workman, C., Tolker-Nielsen, T., & Smets, B. F. (2014). Colony morphology and transcriptome profiling of Pseudomonas putida KT2440 and its mutants deficient in alginate or all EPS synthesis under controlled matric potentials. MicrobiologyOpen, 3(4), 457-469. https://doi.org/10.1002/mbo3.180

Vancouver

Gulez G, Altintas A, Fazli M, Dechesne A, Workman C, Tolker-Nielsen T et al. Colony morphology and transcriptome profiling of Pseudomonas putida KT2440 and its mutants deficient in alginate or all EPS synthesis under controlled matric potentials. MicrobiologyOpen. 2014;3(4):457-469. https://doi.org/10.1002/mbo3.180

Author

Gulez, Gamze ; Altintas, Ali ; Fazli, Mustafa ; Dechesne, Arnaud ; Workman, Christopher ; Tolker-Nielsen, Tim ; Smets, Barth F. / Colony morphology and transcriptome profiling of Pseudomonas putida KT2440 and its mutants deficient in alginate or all EPS synthesis under controlled matric potentials. In: MicrobiologyOpen. 2014 ; Vol. 3, No. 4. pp. 457-469.

Bibtex

@article{144617062ce44ee184a9eac11f38a4ce,
title = "Colony morphology and transcriptome profiling of Pseudomonas putida KT2440 and its mutants deficient in alginate or all EPS synthesis under controlled matric potentials",
abstract = "Pseudomonas putida is a versatile bacterial species adapted to soil and its fluctuations. Like many other species living in soil, P. putida often faces water limitation. Alginate, an exopolysaccharide (EPS) produced by P. putida, is known to create hydrated environments and alleviate the effect of water limitation. In addition to alginate, P. putida is capable of producing cellulose (bcs), putida exopolysaccharide a (pea), and putida exopolysaccharide b (peb). However, unlike alginate, not much is known about their roles under water limitation. Hence, in this study we examined the role of different EPS components under mild water limitation. To create environmentally realistic water limited conditions as observed in soil, we used the Pressurized Porous Surface Model. Our main hypothesis was that under water limitation and in the absence of alginate other exopolysaccharides would be more active to maintain homeostasis. To test our hypothesis, we investigated colony morphologies and whole genome transcriptomes of P. putida KT2440 wild type and its mutants deficient in synthesis of either alginate or all known EPS. Overall our results support that alginate is an important exopolysaccharide under water limitation and in the absence of alginate other tolerance mechanisms are activated.",
author = "Gamze Gulez and Ali Altintas and Mustafa Fazli and Arnaud Dechesne and Christopher Workman and Tim Tolker-Nielsen and Smets, {Barth F.}",
note = "{\textcopyright} 2014 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.",
year = "2014",
doi = "10.1002/mbo3.180",
language = "English",
volume = "3",
pages = "457--469",
journal = "MicrobiologyOpen",
issn = "2045-8827",
publisher = "JohnWiley & Sons Ltd",
number = "4",

}

RIS

TY - JOUR

T1 - Colony morphology and transcriptome profiling of Pseudomonas putida KT2440 and its mutants deficient in alginate or all EPS synthesis under controlled matric potentials

AU - Gulez, Gamze

AU - Altintas, Ali

AU - Fazli, Mustafa

AU - Dechesne, Arnaud

AU - Workman, Christopher

AU - Tolker-Nielsen, Tim

AU - Smets, Barth F.

N1 - © 2014 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.

PY - 2014

Y1 - 2014

N2 - Pseudomonas putida is a versatile bacterial species adapted to soil and its fluctuations. Like many other species living in soil, P. putida often faces water limitation. Alginate, an exopolysaccharide (EPS) produced by P. putida, is known to create hydrated environments and alleviate the effect of water limitation. In addition to alginate, P. putida is capable of producing cellulose (bcs), putida exopolysaccharide a (pea), and putida exopolysaccharide b (peb). However, unlike alginate, not much is known about their roles under water limitation. Hence, in this study we examined the role of different EPS components under mild water limitation. To create environmentally realistic water limited conditions as observed in soil, we used the Pressurized Porous Surface Model. Our main hypothesis was that under water limitation and in the absence of alginate other exopolysaccharides would be more active to maintain homeostasis. To test our hypothesis, we investigated colony morphologies and whole genome transcriptomes of P. putida KT2440 wild type and its mutants deficient in synthesis of either alginate or all known EPS. Overall our results support that alginate is an important exopolysaccharide under water limitation and in the absence of alginate other tolerance mechanisms are activated.

AB - Pseudomonas putida is a versatile bacterial species adapted to soil and its fluctuations. Like many other species living in soil, P. putida often faces water limitation. Alginate, an exopolysaccharide (EPS) produced by P. putida, is known to create hydrated environments and alleviate the effect of water limitation. In addition to alginate, P. putida is capable of producing cellulose (bcs), putida exopolysaccharide a (pea), and putida exopolysaccharide b (peb). However, unlike alginate, not much is known about their roles under water limitation. Hence, in this study we examined the role of different EPS components under mild water limitation. To create environmentally realistic water limited conditions as observed in soil, we used the Pressurized Porous Surface Model. Our main hypothesis was that under water limitation and in the absence of alginate other exopolysaccharides would be more active to maintain homeostasis. To test our hypothesis, we investigated colony morphologies and whole genome transcriptomes of P. putida KT2440 wild type and its mutants deficient in synthesis of either alginate or all known EPS. Overall our results support that alginate is an important exopolysaccharide under water limitation and in the absence of alginate other tolerance mechanisms are activated.

U2 - 10.1002/mbo3.180

DO - 10.1002/mbo3.180

M3 - Journal article

C2 - 24912454

VL - 3

SP - 457

EP - 469

JO - MicrobiologyOpen

JF - MicrobiologyOpen

SN - 2045-8827

IS - 4

ER -

ID: 129018530