Combination Therapy Strategy of Quorum Quenching Enzyme and Quorum Sensing Inhibitor in Suppressing Multiple Quorum Sensing Pathways of P. aeruginosa

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Combination Therapy Strategy of Quorum Quenching Enzyme and Quorum Sensing Inhibitor in Suppressing Multiple Quorum Sensing Pathways of P. aeruginosa. / Fong, July; Zhang, Chaodong; Yang, Renliang; Boo, Zhao Zhi; Tan, Soon Keat; Nielsen, Thomas E.; Givskov, Michael; Liu, Xue-Wei; Bin, Wu; Su, Haibin; Yang, Liang.

In: Scientific Reports, Vol. 8, 1155, 01.12.2018.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Fong, J, Zhang, C, Yang, R, Boo, ZZ, Tan, SK, Nielsen, TE, Givskov, M, Liu, X-W, Bin, W, Su, H & Yang, L 2018, 'Combination Therapy Strategy of Quorum Quenching Enzyme and Quorum Sensing Inhibitor in Suppressing Multiple Quorum Sensing Pathways of P. aeruginosa', Scientific Reports, vol. 8, 1155. https://doi.org/10.1038/s41598-018-19504-w

APA

Fong, J., Zhang, C., Yang, R., Boo, Z. Z., Tan, S. K., Nielsen, T. E., Givskov, M., Liu, X-W., Bin, W., Su, H., & Yang, L. (2018). Combination Therapy Strategy of Quorum Quenching Enzyme and Quorum Sensing Inhibitor in Suppressing Multiple Quorum Sensing Pathways of P. aeruginosa. Scientific Reports, 8, [1155]. https://doi.org/10.1038/s41598-018-19504-w

Vancouver

Fong J, Zhang C, Yang R, Boo ZZ, Tan SK, Nielsen TE et al. Combination Therapy Strategy of Quorum Quenching Enzyme and Quorum Sensing Inhibitor in Suppressing Multiple Quorum Sensing Pathways of P. aeruginosa. Scientific Reports. 2018 Dec 1;8. 1155. https://doi.org/10.1038/s41598-018-19504-w

Author

Fong, July ; Zhang, Chaodong ; Yang, Renliang ; Boo, Zhao Zhi ; Tan, Soon Keat ; Nielsen, Thomas E. ; Givskov, Michael ; Liu, Xue-Wei ; Bin, Wu ; Su, Haibin ; Yang, Liang. / Combination Therapy Strategy of Quorum Quenching Enzyme and Quorum Sensing Inhibitor in Suppressing Multiple Quorum Sensing Pathways of P. aeruginosa. In: Scientific Reports. 2018 ; Vol. 8.

Bibtex

@article{bb4ac92a06d4476ea6890e1ce20612fe,
title = "Combination Therapy Strategy of Quorum Quenching Enzyme and Quorum Sensing Inhibitor in Suppressing Multiple Quorum Sensing Pathways of P. aeruginosa",
abstract = "The threat of antibiotic resistant bacteria has called for alternative antimicrobial strategies that would mitigate the increase of classical resistance mechanism. Many bacteria employ quorum sensing (QS) to govern the production of virulence factors and formation of drug-resistant biofilms. Targeting the mechanism of QS has proven to be a functional alternative to conventional antibiotic control of infections. However, the presence of multiple QS systems in individual bacterial species poses a challenge to this approach. Quorum sensing inhibitors (QSI) and quorum quenching enzymes (QQE) have been both investigated for their QS interfering capabilities. Here, we first simulated the combination effect of QQE and QSI in blocking bacterial QS. The effect was next validated by experiments using AiiA as QQE and G1 as QSI on Pseudomonas aeruginosa LasR/I and RhlR/I QS circuits. Combination of QQE and QSI almost completely blocked the P. aeruginosa las and rhl QS systems. Our findings provide a potential chemical biology application strategy for bacterial QS disruption.",
author = "July Fong and Chaodong Zhang and Renliang Yang and Boo, {Zhao Zhi} and Tan, {Soon Keat} and Nielsen, {Thomas E.} and Michael Givskov and Xue-Wei Liu and Wu Bin and Haibin Su and Liang Yang",
year = "2018",
month = dec,
day = "1",
doi = "10.1038/s41598-018-19504-w",
language = "English",
volume = "8",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Combination Therapy Strategy of Quorum Quenching Enzyme and Quorum Sensing Inhibitor in Suppressing Multiple Quorum Sensing Pathways of P. aeruginosa

AU - Fong, July

AU - Zhang, Chaodong

AU - Yang, Renliang

AU - Boo, Zhao Zhi

AU - Tan, Soon Keat

AU - Nielsen, Thomas E.

AU - Givskov, Michael

AU - Liu, Xue-Wei

AU - Bin, Wu

AU - Su, Haibin

AU - Yang, Liang

PY - 2018/12/1

Y1 - 2018/12/1

N2 - The threat of antibiotic resistant bacteria has called for alternative antimicrobial strategies that would mitigate the increase of classical resistance mechanism. Many bacteria employ quorum sensing (QS) to govern the production of virulence factors and formation of drug-resistant biofilms. Targeting the mechanism of QS has proven to be a functional alternative to conventional antibiotic control of infections. However, the presence of multiple QS systems in individual bacterial species poses a challenge to this approach. Quorum sensing inhibitors (QSI) and quorum quenching enzymes (QQE) have been both investigated for their QS interfering capabilities. Here, we first simulated the combination effect of QQE and QSI in blocking bacterial QS. The effect was next validated by experiments using AiiA as QQE and G1 as QSI on Pseudomonas aeruginosa LasR/I and RhlR/I QS circuits. Combination of QQE and QSI almost completely blocked the P. aeruginosa las and rhl QS systems. Our findings provide a potential chemical biology application strategy for bacterial QS disruption.

AB - The threat of antibiotic resistant bacteria has called for alternative antimicrobial strategies that would mitigate the increase of classical resistance mechanism. Many bacteria employ quorum sensing (QS) to govern the production of virulence factors and formation of drug-resistant biofilms. Targeting the mechanism of QS has proven to be a functional alternative to conventional antibiotic control of infections. However, the presence of multiple QS systems in individual bacterial species poses a challenge to this approach. Quorum sensing inhibitors (QSI) and quorum quenching enzymes (QQE) have been both investigated for their QS interfering capabilities. Here, we first simulated the combination effect of QQE and QSI in blocking bacterial QS. The effect was next validated by experiments using AiiA as QQE and G1 as QSI on Pseudomonas aeruginosa LasR/I and RhlR/I QS circuits. Combination of QQE and QSI almost completely blocked the P. aeruginosa las and rhl QS systems. Our findings provide a potential chemical biology application strategy for bacterial QS disruption.

U2 - 10.1038/s41598-018-19504-w

DO - 10.1038/s41598-018-19504-w

M3 - Journal article

C2 - 29348452

VL - 8

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 1155

ER -

ID: 189106922