Delayed neutrophil recruitment allows nascent Staphylococcus aureus biofilm formation and immune evasion

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Delayed neutrophil recruitment allows nascent Staphylococcus aureus biofilm formation and immune evasion. / Pettygrove, Brian A.; Kratofil, Rachel M.; Alhede, Maria; Jensen, Peter; Newton, Michelle; Qvortrup, Klaus; Pallister, Kyler B.; Bjarnsholt, Thomas; Kubes, Paul; Voyich, Jovanka M.; Stewart, Philip S.

In: Biomaterials, Vol. 275, 120775, 08.2021.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Pettygrove, BA, Kratofil, RM, Alhede, M, Jensen, P, Newton, M, Qvortrup, K, Pallister, KB, Bjarnsholt, T, Kubes, P, Voyich, JM & Stewart, PS 2021, 'Delayed neutrophil recruitment allows nascent Staphylococcus aureus biofilm formation and immune evasion', Biomaterials, vol. 275, 120775. https://doi.org/10.1016/j.biomaterials.2021.120775

APA

Pettygrove, B. A., Kratofil, R. M., Alhede, M., Jensen, P., Newton, M., Qvortrup, K., Pallister, K. B., Bjarnsholt, T., Kubes, P., Voyich, J. M., & Stewart, P. S. (2021). Delayed neutrophil recruitment allows nascent Staphylococcus aureus biofilm formation and immune evasion. Biomaterials, 275, [120775]. https://doi.org/10.1016/j.biomaterials.2021.120775

Vancouver

Pettygrove BA, Kratofil RM, Alhede M, Jensen P, Newton M, Qvortrup K et al. Delayed neutrophil recruitment allows nascent Staphylococcus aureus biofilm formation and immune evasion. Biomaterials. 2021 Aug;275. 120775. https://doi.org/10.1016/j.biomaterials.2021.120775

Author

Pettygrove, Brian A. ; Kratofil, Rachel M. ; Alhede, Maria ; Jensen, Peter ; Newton, Michelle ; Qvortrup, Klaus ; Pallister, Kyler B. ; Bjarnsholt, Thomas ; Kubes, Paul ; Voyich, Jovanka M. ; Stewart, Philip S. / Delayed neutrophil recruitment allows nascent Staphylococcus aureus biofilm formation and immune evasion. In: Biomaterials. 2021 ; Vol. 275.

Bibtex

@article{6c869eabb353481a80f71b72eb45ab02,
title = "Delayed neutrophil recruitment allows nascent Staphylococcus aureus biofilm formation and immune evasion",
abstract = "Biofilms that form on implanted medical devices cause recalcitrant infections. The early events enabling contaminating bacteria to evade immune clearance, before a mature biofilm is established, are poorly understood. Live imaging in vitro demonstrated that Staphylococcus aureus sparsely inoculated on an abiotic surface can go undiscovered by human neutrophils, grow, and form aggregates. Small (~50 μm2) aggregates of attached bacteria resisted killing by human neutrophils, resulting in neutrophil lysis and bacterial persistence. In vivo, neutrophil recruitment to a peritoneal implant was spatially heterogenous, with some bacterial aggregates remaining undiscovered by neutrophils after 24 h. Intravital imaging in mouse skin revealed that attached S. aureus aggregates grew and remained undiscovered by neutrophils for up to 3 h. These results suggest a model in which delayed recruitment of neutrophils to an abiotic implant presents a critical window in which bacteria establish a nascent biofilm and acquire tolerance to neutrophil killing.",
keywords = "Biofilm, Implant-associated infection, Microscopy, Neutrophil, Staphylococcus aureus, Up to 10)",
author = "Pettygrove, {Brian A.} and Kratofil, {Rachel M.} and Maria Alhede and Peter Jensen and Michelle Newton and Klaus Qvortrup and Pallister, {Kyler B.} and Thomas Bjarnsholt and Paul Kubes and Voyich, {Jovanka M.} and Stewart, {Philip S.}",
note = "Publisher Copyright: {\textcopyright} 2021 Elsevier Ltd",
year = "2021",
month = aug,
doi = "10.1016/j.biomaterials.2021.120775",
language = "English",
volume = "275",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Delayed neutrophil recruitment allows nascent Staphylococcus aureus biofilm formation and immune evasion

AU - Pettygrove, Brian A.

AU - Kratofil, Rachel M.

AU - Alhede, Maria

AU - Jensen, Peter

AU - Newton, Michelle

AU - Qvortrup, Klaus

AU - Pallister, Kyler B.

AU - Bjarnsholt, Thomas

AU - Kubes, Paul

AU - Voyich, Jovanka M.

AU - Stewart, Philip S.

N1 - Publisher Copyright: © 2021 Elsevier Ltd

PY - 2021/8

Y1 - 2021/8

N2 - Biofilms that form on implanted medical devices cause recalcitrant infections. The early events enabling contaminating bacteria to evade immune clearance, before a mature biofilm is established, are poorly understood. Live imaging in vitro demonstrated that Staphylococcus aureus sparsely inoculated on an abiotic surface can go undiscovered by human neutrophils, grow, and form aggregates. Small (~50 μm2) aggregates of attached bacteria resisted killing by human neutrophils, resulting in neutrophil lysis and bacterial persistence. In vivo, neutrophil recruitment to a peritoneal implant was spatially heterogenous, with some bacterial aggregates remaining undiscovered by neutrophils after 24 h. Intravital imaging in mouse skin revealed that attached S. aureus aggregates grew and remained undiscovered by neutrophils for up to 3 h. These results suggest a model in which delayed recruitment of neutrophils to an abiotic implant presents a critical window in which bacteria establish a nascent biofilm and acquire tolerance to neutrophil killing.

AB - Biofilms that form on implanted medical devices cause recalcitrant infections. The early events enabling contaminating bacteria to evade immune clearance, before a mature biofilm is established, are poorly understood. Live imaging in vitro demonstrated that Staphylococcus aureus sparsely inoculated on an abiotic surface can go undiscovered by human neutrophils, grow, and form aggregates. Small (~50 μm2) aggregates of attached bacteria resisted killing by human neutrophils, resulting in neutrophil lysis and bacterial persistence. In vivo, neutrophil recruitment to a peritoneal implant was spatially heterogenous, with some bacterial aggregates remaining undiscovered by neutrophils after 24 h. Intravital imaging in mouse skin revealed that attached S. aureus aggregates grew and remained undiscovered by neutrophils for up to 3 h. These results suggest a model in which delayed recruitment of neutrophils to an abiotic implant presents a critical window in which bacteria establish a nascent biofilm and acquire tolerance to neutrophil killing.

KW - Biofilm

KW - Implant-associated infection

KW - Microscopy

KW - Neutrophil

KW - Staphylococcus aureus

KW - Up to 10)

U2 - 10.1016/j.biomaterials.2021.120775

DO - 10.1016/j.biomaterials.2021.120775

M3 - Journal article

C2 - 34243039

AN - SCOPUS:85109215098

VL - 275

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

M1 - 120775

ER -

ID: 274569161