Dispersed cells represent a distinct stage in the transition from bacterial biofilm to planktonic lifestyles
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Dispersed cells represent a distinct stage in the transition from bacterial biofilm to planktonic lifestyles. / Chua, Song Lin; Liu, Yang; Yam, Joey Kuok Hoong; Chen, Yicai; Vejborg, Rebecca Munk; Tan, Bryan Giin Chyuan; Kjelleberg, Staffan; Tolker-Nielsen, Tim; Givskov, Michael; Yang, Liang.
In: Nature Communications, Vol. 5, 4462, 2014, p. 1-12.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Dispersed cells represent a distinct stage in the transition from bacterial biofilm to planktonic lifestyles
AU - Chua, Song Lin
AU - Liu, Yang
AU - Yam, Joey Kuok Hoong
AU - Chen, Yicai
AU - Vejborg, Rebecca Munk
AU - Tan, Bryan Giin Chyuan
AU - Kjelleberg, Staffan
AU - Tolker-Nielsen, Tim
AU - Givskov, Michael
AU - Yang, Liang
PY - 2014
Y1 - 2014
N2 - Bacteria assume distinct lifestyles during the planktonic and biofilm modes of growth. Increased levels of the intracellular messenger c-di-GMP determine the transition from planktonic to biofilm growth, while a reduction causes biofilm dispersal. It is generally assumed that cells dispersed from biofilms immediately go into the planktonic growth phase. Here we use single-nucleotide resolution transcriptomic analysis to show that the physiology of dispersed cells from Pseudomonas aeruginosa biofilms is highly different from those of planktonic and biofilm cells. In dispersed cells, the expression of the small regulatory RNAs RsmY and RsmZ is downregulated, whereas secretion genes are induced. Dispersed cells are highly virulent against macrophages and Caenorhabditis elegans compared with planktonic cells. In addition, they are highly sensitive towards iron stress, and the combination of a biofilm-dispersing agent, an iron chelator and tobramycin efficiently reduces the survival of the dispersed cells.
AB - Bacteria assume distinct lifestyles during the planktonic and biofilm modes of growth. Increased levels of the intracellular messenger c-di-GMP determine the transition from planktonic to biofilm growth, while a reduction causes biofilm dispersal. It is generally assumed that cells dispersed from biofilms immediately go into the planktonic growth phase. Here we use single-nucleotide resolution transcriptomic analysis to show that the physiology of dispersed cells from Pseudomonas aeruginosa biofilms is highly different from those of planktonic and biofilm cells. In dispersed cells, the expression of the small regulatory RNAs RsmY and RsmZ is downregulated, whereas secretion genes are induced. Dispersed cells are highly virulent against macrophages and Caenorhabditis elegans compared with planktonic cells. In addition, they are highly sensitive towards iron stress, and the combination of a biofilm-dispersing agent, an iron chelator and tobramycin efficiently reduces the survival of the dispersed cells.
U2 - 10.1038/ncomms5462
DO - 10.1038/ncomms5462
M3 - Journal article
C2 - 25042103
VL - 5
SP - 1
EP - 12
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
M1 - 4462
ER -
ID: 129018466