Early implant-associated osteomyelitis results in a peri-implanted bacterial reservoir

Research output: Contribution to journalJournal articlepeer-review

Standard

Early implant-associated osteomyelitis results in a peri-implanted bacterial reservoir. / Jensen, Louise Kruse; Koch, Janne; Aalbæk, Bent; Moodley, Arshnee; Bjarnsholt, Thomas; Kragh, Kasper Nørskov; Petersen, Andreas; Jensen, Henrik Elvang.

In: APMIS - Journal of Pathology, Microbiology and Immunology, Vol. 125, No. 1, 01.2017, p. 38-45.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Jensen, LK, Koch, J, Aalbæk, B, Moodley, A, Bjarnsholt, T, Kragh, KN, Petersen, A & Jensen, HE 2017, 'Early implant-associated osteomyelitis results in a peri-implanted bacterial reservoir', APMIS - Journal of Pathology, Microbiology and Immunology, vol. 125, no. 1, pp. 38-45. https://doi.org/10.1111/apm.12597

APA

Jensen, L. K., Koch, J., Aalbæk, B., Moodley, A., Bjarnsholt, T., Kragh, K. N., Petersen, A., & Jensen, H. E. (2017). Early implant-associated osteomyelitis results in a peri-implanted bacterial reservoir. APMIS - Journal of Pathology, Microbiology and Immunology, 125(1), 38-45. https://doi.org/10.1111/apm.12597

Vancouver

Jensen LK, Koch J, Aalbæk B, Moodley A, Bjarnsholt T, Kragh KN et al. Early implant-associated osteomyelitis results in a peri-implanted bacterial reservoir. APMIS - Journal of Pathology, Microbiology and Immunology. 2017 Jan;125(1):38-45. https://doi.org/10.1111/apm.12597

Author

Jensen, Louise Kruse ; Koch, Janne ; Aalbæk, Bent ; Moodley, Arshnee ; Bjarnsholt, Thomas ; Kragh, Kasper Nørskov ; Petersen, Andreas ; Jensen, Henrik Elvang. / Early implant-associated osteomyelitis results in a peri-implanted bacterial reservoir. In: APMIS - Journal of Pathology, Microbiology and Immunology. 2017 ; Vol. 125, No. 1. pp. 38-45.

Bibtex

@article{41c84521af4e4cb39c35718446f4fb27,
title = "Early implant-associated osteomyelitis results in a peri-implanted bacterial reservoir",
abstract = "Implant-associated osteomyelitis (IAO) is a common complication in orthopedic surgery. The aim of this study was to elucidate how deep IAO can go into the peri-implanted bone tissue within a week. The study was performed in a porcine model of IAO. A small steel implant and either 104 CFU/kg body weight of Staphylococcus aureus or saline was inserted into the right tibial bone of 12 pigs. The animals were consecutively killed on day 2, 4 and 6 following implantation. Bone tissue around the implant was histologically evaluated. Identification of S. aureus was performed immunohistochemically on tissue section and with scanning electron microscopy and peptide nucleic acid in situ hybridization on implants. The distance of the peri-implanted pathological bone area (PIBA), measured perpendicular to the implant, was significantly larger in infected animals compared to controls (p = 0.0014). The largest differences were seen after 4 and 6 days of inoculation, where PIBA measurements of up to 6 mm were observed. Positive S. aureus bacteria were identified on implants and from 25 μm to 6 mm into PIBA. This is important knowledge for optimizing outcomes of surgical debridement in osteomyelitis.",
author = "Jensen, {Louise Kruse} and Janne Koch and Bent Aalb{\ae}k and Arshnee Moodley and Thomas Bjarnsholt and Kragh, {Kasper N{\o}rskov} and Andreas Petersen and Jensen, {Henrik Elvang}",
year = "2017",
month = jan,
doi = "10.1111/apm.12597",
language = "English",
volume = "125",
pages = "38--45",
journal = "A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica",
issn = "0903-4641",
publisher = "Wiley Online",
number = "1",

}

RIS

TY - JOUR

T1 - Early implant-associated osteomyelitis results in a peri-implanted bacterial reservoir

AU - Jensen, Louise Kruse

AU - Koch, Janne

AU - Aalbæk, Bent

AU - Moodley, Arshnee

AU - Bjarnsholt, Thomas

AU - Kragh, Kasper Nørskov

AU - Petersen, Andreas

AU - Jensen, Henrik Elvang

PY - 2017/1

Y1 - 2017/1

N2 - Implant-associated osteomyelitis (IAO) is a common complication in orthopedic surgery. The aim of this study was to elucidate how deep IAO can go into the peri-implanted bone tissue within a week. The study was performed in a porcine model of IAO. A small steel implant and either 104 CFU/kg body weight of Staphylococcus aureus or saline was inserted into the right tibial bone of 12 pigs. The animals were consecutively killed on day 2, 4 and 6 following implantation. Bone tissue around the implant was histologically evaluated. Identification of S. aureus was performed immunohistochemically on tissue section and with scanning electron microscopy and peptide nucleic acid in situ hybridization on implants. The distance of the peri-implanted pathological bone area (PIBA), measured perpendicular to the implant, was significantly larger in infected animals compared to controls (p = 0.0014). The largest differences were seen after 4 and 6 days of inoculation, where PIBA measurements of up to 6 mm were observed. Positive S. aureus bacteria were identified on implants and from 25 μm to 6 mm into PIBA. This is important knowledge for optimizing outcomes of surgical debridement in osteomyelitis.

AB - Implant-associated osteomyelitis (IAO) is a common complication in orthopedic surgery. The aim of this study was to elucidate how deep IAO can go into the peri-implanted bone tissue within a week. The study was performed in a porcine model of IAO. A small steel implant and either 104 CFU/kg body weight of Staphylococcus aureus or saline was inserted into the right tibial bone of 12 pigs. The animals were consecutively killed on day 2, 4 and 6 following implantation. Bone tissue around the implant was histologically evaluated. Identification of S. aureus was performed immunohistochemically on tissue section and with scanning electron microscopy and peptide nucleic acid in situ hybridization on implants. The distance of the peri-implanted pathological bone area (PIBA), measured perpendicular to the implant, was significantly larger in infected animals compared to controls (p = 0.0014). The largest differences were seen after 4 and 6 days of inoculation, where PIBA measurements of up to 6 mm were observed. Positive S. aureus bacteria were identified on implants and from 25 μm to 6 mm into PIBA. This is important knowledge for optimizing outcomes of surgical debridement in osteomyelitis.

U2 - 10.1111/apm.12597

DO - 10.1111/apm.12597

M3 - Journal article

C2 - 27704604

VL - 125

SP - 38

EP - 45

JO - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

JF - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

SN - 0903-4641

IS - 1

ER -

ID: 167353757