Effects of ginseng on Pseudomonas aeruginosa motility and biofilm formation
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Effects of ginseng on Pseudomonas aeruginosa motility and biofilm formation. / Wu, Hong; Lee, Baoleri; Yang, Liang; Wang, Hengzuang; Givskov, Michael; Molin, Søren; Høiby, Niels; Song, Zhijun.
In: F E M S Immunology and Medical Microbiology, Vol. 62, No. 1, 01.06.2011, p. 49-56.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Effects of ginseng on Pseudomonas aeruginosa motility and biofilm formation
AU - Wu, Hong
AU - Lee, Baoleri
AU - Yang, Liang
AU - Wang, Hengzuang
AU - Givskov, Michael
AU - Molin, Søren
AU - Høiby, Niels
AU - Song, Zhijun
N1 - © 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
PY - 2011/6/1
Y1 - 2011/6/1
N2 - Biofilm-associated chronic Pseudomonas aeruginosa lung infections in patients with cystic fibrosis are virtually impossible to eradicate with antibiotics because biofilm-growing bacteria are highly tolerant to antibiotics and host defense mechanisms. Previously, we found that ginseng treatments protected animal models from developing chronic lung infection by P. aeruginosa. In the present study, the effects of ginseng on the formation of P. aeruginosa biofilms were further investigated in vitro and in vivo. Ginseng aqueous extract at concentrations of 0.5-2.0% did not inhibit the growth of P. aeruginosa, but significantly prevented P. aeruginosa from forming biofilm. Exposure to 0.5% ginseng aqueous extract for 24 h destroyed most 7-day-old mature biofilms formed by both mucoid and nonmucoid P. aeruginosa strains. Ginseng treatment enhanced swimming and twitching motility, but reduced swarming of P. aeruginosa at concentrations as low as 0.25%. Oral administration of ginseng extracts in mice promoted phagocytosis of P. aeruginosa PAO1 by airway phagocytes, but did not affect phagocytosis of a PAO1-filM mutant. Our study suggests that ginseng treatment may help to eradicate the biofilm-associated chronic infections caused by P. aeruginosa.
AB - Biofilm-associated chronic Pseudomonas aeruginosa lung infections in patients with cystic fibrosis are virtually impossible to eradicate with antibiotics because biofilm-growing bacteria are highly tolerant to antibiotics and host defense mechanisms. Previously, we found that ginseng treatments protected animal models from developing chronic lung infection by P. aeruginosa. In the present study, the effects of ginseng on the formation of P. aeruginosa biofilms were further investigated in vitro and in vivo. Ginseng aqueous extract at concentrations of 0.5-2.0% did not inhibit the growth of P. aeruginosa, but significantly prevented P. aeruginosa from forming biofilm. Exposure to 0.5% ginseng aqueous extract for 24 h destroyed most 7-day-old mature biofilms formed by both mucoid and nonmucoid P. aeruginosa strains. Ginseng treatment enhanced swimming and twitching motility, but reduced swarming of P. aeruginosa at concentrations as low as 0.25%. Oral administration of ginseng extracts in mice promoted phagocytosis of P. aeruginosa PAO1 by airway phagocytes, but did not affect phagocytosis of a PAO1-filM mutant. Our study suggests that ginseng treatment may help to eradicate the biofilm-associated chronic infections caused by P. aeruginosa.
KW - Animals
KW - Biofilms
KW - Cell Movement
KW - Cystic Fibrosis
KW - Humans
KW - Mice
KW - Panax
KW - Phagocytosis
KW - Plant Extracts
KW - Pseudomonas Infections
KW - Pseudomonas aeruginosa
KW - Treatment Outcome
U2 - 10.1111/j.1574-695X.2011.00787.x
DO - 10.1111/j.1574-695X.2011.00787.x
M3 - Journal article
C2 - 21303421
VL - 62
SP - 49
EP - 56
JO - Pathogens and Disease
JF - Pathogens and Disease
SN - 2049-632X
IS - 1
ER -
ID: 38230083