Effects of iron on DNA release and biofilm development by Pseudomonas aeruginosa
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Effects of iron on DNA release and biofilm development by Pseudomonas aeruginosa. / Yang, Liang; Barken, Kim B; Skindersoe, Mette E; Christensen, Allan B; Givskov, Michael; Tolker-Nielsen, Tim.
In: Microbiology, Vol. 153, No. Pt 5, 2007, p. 1318-28.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Effects of iron on DNA release and biofilm development by Pseudomonas aeruginosa
AU - Yang, Liang
AU - Barken, Kim B
AU - Skindersoe, Mette E
AU - Christensen, Allan B
AU - Givskov, Michael
AU - Tolker-Nielsen, Tim
N1 - Keywords: Biofilms; DNA, Bacterial; Gene Expression Regulation; Genes, Reporter; Green Fluorescent Proteins; Iron; Operon; Pseudomonas aeruginosa; Quorum Sensing; Signal Transduction
PY - 2007
Y1 - 2007
N2 - Extracellular DNA is one of the major matrix components in Pseudomonas aeruginosa biofilms. It functions as an intercellular connector and plays a role in stabilization of the biofilms. Evidence that DNA release in P. aeruginosa PAO1 biofilms is controlled by the las-rhl and pqs quorum-sensing systems has been previously presented. This paper provides evidence that DNA release in P. aeruginosa PAO1 biofilms is also under iron regulation. Experiments involving cultivation of P. aeruginosa in microtitre trays suggested that pqs expression, DNA release and biofilm formation were favoured in media with low iron concentrations (5 microM FeCl(3)), and decreased with increasing iron concentrations. Experiments involving cultivation of P. aeruginosa in a flow-chamber system suggested that a high level of iron (100 microM FeCl(3)) in the medium suppressed DNA release, structural biofilm development, and the development of subpopulations with increased tolerance toward antimicrobial compounds. Experiments with P. aeruginosa strains harbouring fluorescent reporters suggested that expression of the pqs operon was induced in particular subpopulations of the biofilm cells under low-iron conditions (1 microM FeCl(3)), but repressed in the biofilm cells under high-iron conditions (100 microM FeCl(3)).
AB - Extracellular DNA is one of the major matrix components in Pseudomonas aeruginosa biofilms. It functions as an intercellular connector and plays a role in stabilization of the biofilms. Evidence that DNA release in P. aeruginosa PAO1 biofilms is controlled by the las-rhl and pqs quorum-sensing systems has been previously presented. This paper provides evidence that DNA release in P. aeruginosa PAO1 biofilms is also under iron regulation. Experiments involving cultivation of P. aeruginosa in microtitre trays suggested that pqs expression, DNA release and biofilm formation were favoured in media with low iron concentrations (5 microM FeCl(3)), and decreased with increasing iron concentrations. Experiments involving cultivation of P. aeruginosa in a flow-chamber system suggested that a high level of iron (100 microM FeCl(3)) in the medium suppressed DNA release, structural biofilm development, and the development of subpopulations with increased tolerance toward antimicrobial compounds. Experiments with P. aeruginosa strains harbouring fluorescent reporters suggested that expression of the pqs operon was induced in particular subpopulations of the biofilm cells under low-iron conditions (1 microM FeCl(3)), but repressed in the biofilm cells under high-iron conditions (100 microM FeCl(3)).
U2 - 10.1099/mic.0.2006/004911-0
DO - 10.1099/mic.0.2006/004911-0
M3 - Journal article
C2 - 17464046
VL - 153
SP - 1318
EP - 1328
JO - Microbiology
JF - Microbiology
SN - 1350-0872
IS - Pt 5
ER -
ID: 10613610