Effects of Photoactivated Titanium Dioxide Nanopowders and Coating on Planktonic and Biofilm Growth of Pseudomonas aeruginosa
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Effects of Photoactivated Titanium Dioxide Nanopowders and Coating on Planktonic and Biofilm Growth of Pseudomonas aeruginosa. / Polo, Andrea; Diamanti, Maria Vittoria; Bjarnsholt, Thomas; Høiby, Niels; Villa, Federica; Pedeferri, Maria Pia; Cappitelli, Francesca.
In: Photochemistry and Photobiology, 25.07.2011.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Effects of Photoactivated Titanium Dioxide Nanopowders and Coating on Planktonic and Biofilm Growth of Pseudomonas aeruginosa
AU - Polo, Andrea
AU - Diamanti, Maria Vittoria
AU - Bjarnsholt, Thomas
AU - Høiby, Niels
AU - Villa, Federica
AU - Pedeferri, Maria Pia
AU - Cappitelli, Francesca
N1 - Photochemistry and Photobiology © 2011 American Society for Photobiology.
PY - 2011/7/25
Y1 - 2011/7/25
N2 - We exploited the ability of photocatalytic titanium dioxide (TiO(2) ) as an agent for the biofilm control. Two photocatalytic systems were investigated: a 3g/l suspension of TiO(2) nanopowder in demineralised water and glass slides coated with a TiO(2) thin film, achieved by sol-gel deposition. A running protocol for the photoactivation of TiO(2) was set up using the dye rhodamine B. The microorganisms studied were Pseudomonas stutzeri, Pseudomonas aeruginosa and a Bacillus cereus-group as planktonic cells. P. aeruginosa biofilms were also studied at both the solid-liquid and the solid-air interface. The TiO(2) nanopowder produced 1-log reduction of Bacillus sp. planktonic cells in 24 h, 2-log reduction of P. stutzeri planktonic cells in 30 min and 1-log reduction of P. aeruginosa planktonic cells in 2 h compared to non-photoactivated TiO(2) . TiO(2) thin film produced almost a complete eradication of P. aeruginosa planktonic cells (initial concentration 10(8) cells/ml) in 24 h compared to a 3-log reduction caused by UV-A light alone. In contrast, neither the photocatalytic treatment with TiO(2) film nor that with TiO(2) nanopowder had any effect on P. aeruginosa biofilms at all the interfaces investigated. Possible explanations for these findings, and for the discrepancy between this work and literature data, are discussed.
AB - We exploited the ability of photocatalytic titanium dioxide (TiO(2) ) as an agent for the biofilm control. Two photocatalytic systems were investigated: a 3g/l suspension of TiO(2) nanopowder in demineralised water and glass slides coated with a TiO(2) thin film, achieved by sol-gel deposition. A running protocol for the photoactivation of TiO(2) was set up using the dye rhodamine B. The microorganisms studied were Pseudomonas stutzeri, Pseudomonas aeruginosa and a Bacillus cereus-group as planktonic cells. P. aeruginosa biofilms were also studied at both the solid-liquid and the solid-air interface. The TiO(2) nanopowder produced 1-log reduction of Bacillus sp. planktonic cells in 24 h, 2-log reduction of P. stutzeri planktonic cells in 30 min and 1-log reduction of P. aeruginosa planktonic cells in 2 h compared to non-photoactivated TiO(2) . TiO(2) thin film produced almost a complete eradication of P. aeruginosa planktonic cells (initial concentration 10(8) cells/ml) in 24 h compared to a 3-log reduction caused by UV-A light alone. In contrast, neither the photocatalytic treatment with TiO(2) film nor that with TiO(2) nanopowder had any effect on P. aeruginosa biofilms at all the interfaces investigated. Possible explanations for these findings, and for the discrepancy between this work and literature data, are discussed.
U2 - 10.1111/j.1751-1097.2011.00972.x
DO - 10.1111/j.1751-1097.2011.00972.x
M3 - Journal article
C2 - 21790617
JO - Photochemistry and Photobiology
JF - Photochemistry and Photobiology
SN - 0031-8655
ER -
ID: 33910157