Efficacy of a synthetic antimicrobial peptidomimetic versus vancomycin in a Staphylococcus epidermidis device-related murine peritonitis model

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Efficacy of a synthetic antimicrobial peptidomimetic versus vancomycin in a Staphylococcus epidermidis device-related murine peritonitis model. / Cavanagh, Jorunn Pauline; Granslo, Hildegunn Norbakken; Fredheim, Elizabeth Aarag; Christophersen, Lars; Jensen, Peter Østrup; Thomsen, Kim; van Gennip, Maria; Klingenberg, Claus; Flaegstad, Trond; Moser, Claus; Alhede, Maria.

In: The Journal of antimicrobial chemotherapy, Vol. 68, No. 9, 09.2013, p. 2106-10.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Cavanagh, JP, Granslo, HN, Fredheim, EA, Christophersen, L, Jensen, PØ, Thomsen, K, van Gennip, M, Klingenberg, C, Flaegstad, T, Moser, C & Alhede, M 2013, 'Efficacy of a synthetic antimicrobial peptidomimetic versus vancomycin in a Staphylococcus epidermidis device-related murine peritonitis model', The Journal of antimicrobial chemotherapy, vol. 68, no. 9, pp. 2106-10. https://doi.org/10.1093/jac/dkt161

APA

Cavanagh, J. P., Granslo, H. N., Fredheim, E. A., Christophersen, L., Jensen, P. Ø., Thomsen, K., van Gennip, M., Klingenberg, C., Flaegstad, T., Moser, C., & Alhede, M. (2013). Efficacy of a synthetic antimicrobial peptidomimetic versus vancomycin in a Staphylococcus epidermidis device-related murine peritonitis model. The Journal of antimicrobial chemotherapy, 68(9), 2106-10. https://doi.org/10.1093/jac/dkt161

Vancouver

Cavanagh JP, Granslo HN, Fredheim EA, Christophersen L, Jensen PØ, Thomsen K et al. Efficacy of a synthetic antimicrobial peptidomimetic versus vancomycin in a Staphylococcus epidermidis device-related murine peritonitis model. The Journal of antimicrobial chemotherapy. 2013 Sep;68(9):2106-10. https://doi.org/10.1093/jac/dkt161

Author

Cavanagh, Jorunn Pauline ; Granslo, Hildegunn Norbakken ; Fredheim, Elizabeth Aarag ; Christophersen, Lars ; Jensen, Peter Østrup ; Thomsen, Kim ; van Gennip, Maria ; Klingenberg, Claus ; Flaegstad, Trond ; Moser, Claus ; Alhede, Maria. / Efficacy of a synthetic antimicrobial peptidomimetic versus vancomycin in a Staphylococcus epidermidis device-related murine peritonitis model. In: The Journal of antimicrobial chemotherapy. 2013 ; Vol. 68, No. 9. pp. 2106-10.

Bibtex

@article{ac0d427aa7f14753b108004fd94e3406,
title = "Efficacy of a synthetic antimicrobial peptidomimetic versus vancomycin in a Staphylococcus epidermidis device-related murine peritonitis model",
abstract = "Objectives Biofilm-forming Staphylococcus epidermidis is a prevalent cause of peritonitis during peritoneal dialysis. We compared the efficacy of a synthetic antimicrobial peptidomimetic (Ltx21) versus vancomycin in a murine model mimicking a device-related peritonitis. Methods Silicone implants, pre-colonized with an S. epidermidis biofilm, were inserted into the peritoneal cavity of BALB/c mice. Three groups (36 mice in each) with pre-colonized implants received intraperitoneal treatment with Ltx21, vancomycin or placebo. Mice were euthanized on day 3 (n = 12), day 6 (n = 12) or day 8 (n = 12) post-implantation. Controls were mice with sterile implants (n = 18) and mice without surgery (n = 6). Bacterial reductions in cfu were analysed from implants and peritoneal fluid (PF). Inflammatory responses in serum and PF were measured. Results Vancomycin resulted in a stronger reduction in cfu counts, both on pre-colonized implants and in PF, compared with Ltx21 and placebo. Complete bacterial clearance of the implants was not achieved in any of the groups. The implants pre-colonized with S. epidermidis 1457 resulted in a low-grade peritonitis. We observed, only on day 6, a significant increase in the PF leucocyte count in the group with pre-colonized implants compared with the group with sterile implants (P = 0.0364). Conclusions Treatment with vancomycin or Ltx21 was not sufficient to achieve complete bacterial clearance of implants, underlining the difficulties of treating such infections. The low-grade infection may attenuate the inflammatory response and contribute to impaired bacterial clearance.",
keywords = "Animals, Anti-Bacterial Agents, Bacterial Load, Catheter-Related Infections, Disease Models, Animal, Female, Mice, Mice, Inbred BALB C, Peptidomimetics, Peritoneal Dialysis, Peritonitis, Staphylococcal Infections, Staphylococcus epidermidis, Treatment Outcome, Vancomycin",
author = "Cavanagh, {Jorunn Pauline} and Granslo, {Hildegunn Norbakken} and Fredheim, {Elizabeth Aarag} and Lars Christophersen and Jensen, {Peter {\O}strup} and Kim Thomsen and {van Gennip}, Maria and Claus Klingenberg and Trond Flaegstad and Claus Moser and Maria Alhede",
year = "2013",
month = sep,
doi = "10.1093/jac/dkt161",
language = "English",
volume = "68",
pages = "2106--10",
journal = "Journal of Antimicrobial Chemotherapy",
issn = "0305-7453",
publisher = "Oxford University Press",
number = "9",

}

RIS

TY - JOUR

T1 - Efficacy of a synthetic antimicrobial peptidomimetic versus vancomycin in a Staphylococcus epidermidis device-related murine peritonitis model

AU - Cavanagh, Jorunn Pauline

AU - Granslo, Hildegunn Norbakken

AU - Fredheim, Elizabeth Aarag

AU - Christophersen, Lars

AU - Jensen, Peter Østrup

AU - Thomsen, Kim

AU - van Gennip, Maria

AU - Klingenberg, Claus

AU - Flaegstad, Trond

AU - Moser, Claus

AU - Alhede, Maria

PY - 2013/9

Y1 - 2013/9

N2 - Objectives Biofilm-forming Staphylococcus epidermidis is a prevalent cause of peritonitis during peritoneal dialysis. We compared the efficacy of a synthetic antimicrobial peptidomimetic (Ltx21) versus vancomycin in a murine model mimicking a device-related peritonitis. Methods Silicone implants, pre-colonized with an S. epidermidis biofilm, were inserted into the peritoneal cavity of BALB/c mice. Three groups (36 mice in each) with pre-colonized implants received intraperitoneal treatment with Ltx21, vancomycin or placebo. Mice were euthanized on day 3 (n = 12), day 6 (n = 12) or day 8 (n = 12) post-implantation. Controls were mice with sterile implants (n = 18) and mice without surgery (n = 6). Bacterial reductions in cfu were analysed from implants and peritoneal fluid (PF). Inflammatory responses in serum and PF were measured. Results Vancomycin resulted in a stronger reduction in cfu counts, both on pre-colonized implants and in PF, compared with Ltx21 and placebo. Complete bacterial clearance of the implants was not achieved in any of the groups. The implants pre-colonized with S. epidermidis 1457 resulted in a low-grade peritonitis. We observed, only on day 6, a significant increase in the PF leucocyte count in the group with pre-colonized implants compared with the group with sterile implants (P = 0.0364). Conclusions Treatment with vancomycin or Ltx21 was not sufficient to achieve complete bacterial clearance of implants, underlining the difficulties of treating such infections. The low-grade infection may attenuate the inflammatory response and contribute to impaired bacterial clearance.

AB - Objectives Biofilm-forming Staphylococcus epidermidis is a prevalent cause of peritonitis during peritoneal dialysis. We compared the efficacy of a synthetic antimicrobial peptidomimetic (Ltx21) versus vancomycin in a murine model mimicking a device-related peritonitis. Methods Silicone implants, pre-colonized with an S. epidermidis biofilm, were inserted into the peritoneal cavity of BALB/c mice. Three groups (36 mice in each) with pre-colonized implants received intraperitoneal treatment with Ltx21, vancomycin or placebo. Mice were euthanized on day 3 (n = 12), day 6 (n = 12) or day 8 (n = 12) post-implantation. Controls were mice with sterile implants (n = 18) and mice without surgery (n = 6). Bacterial reductions in cfu were analysed from implants and peritoneal fluid (PF). Inflammatory responses in serum and PF were measured. Results Vancomycin resulted in a stronger reduction in cfu counts, both on pre-colonized implants and in PF, compared with Ltx21 and placebo. Complete bacterial clearance of the implants was not achieved in any of the groups. The implants pre-colonized with S. epidermidis 1457 resulted in a low-grade peritonitis. We observed, only on day 6, a significant increase in the PF leucocyte count in the group with pre-colonized implants compared with the group with sterile implants (P = 0.0364). Conclusions Treatment with vancomycin or Ltx21 was not sufficient to achieve complete bacterial clearance of implants, underlining the difficulties of treating such infections. The low-grade infection may attenuate the inflammatory response and contribute to impaired bacterial clearance.

KW - Animals

KW - Anti-Bacterial Agents

KW - Bacterial Load

KW - Catheter-Related Infections

KW - Disease Models, Animal

KW - Female

KW - Mice

KW - Mice, Inbred BALB C

KW - Peptidomimetics

KW - Peritoneal Dialysis

KW - Peritonitis

KW - Staphylococcal Infections

KW - Staphylococcus epidermidis

KW - Treatment Outcome

KW - Vancomycin

U2 - 10.1093/jac/dkt161

DO - 10.1093/jac/dkt161

M3 - Journal article

C2 - 23645587

VL - 68

SP - 2106

EP - 2110

JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

SN - 0305-7453

IS - 9

ER -

ID: 105877305