Elevated c-di-GMP Levels and Expression of the Type III Secretion System Promote Corneal Infection by Pseudomonas aeruginosa

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Elevated c-di-GMP Levels and Expression of the Type III Secretion System Promote Corneal Infection by Pseudomonas aeruginosa. / Yam, Joey Kuok Hoong; Aung, Thet Tun; Chua, Song Lin; Cheng, Yingying; Kohli, Gurjeet Singh; Zhou, Jianuan; Constancias, Florentin; Liu, Yang; Cai, Zhao; Salido, May Margarette Santillan; Drautz-Moses, Daniela I.; Rice, Scott A.; Schuster, Stephan Christoph; Boo, Zhao Zhi; Wu, Bin; Kjelleberg, Staffan; Tolker-Nielsen, Tim; Lakshminarayanan, Rajamani; Beuerman, Roger W.; Yang, Liang; Givskov, Michael.

In: Infection and Immunity, Vol. 90, No. 8, 2022.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Yam, JKH, Aung, TT, Chua, SL, Cheng, Y, Kohli, GS, Zhou, J, Constancias, F, Liu, Y, Cai, Z, Salido, MMS, Drautz-Moses, DI, Rice, SA, Schuster, SC, Boo, ZZ, Wu, B, Kjelleberg, S, Tolker-Nielsen, T, Lakshminarayanan, R, Beuerman, RW, Yang, L & Givskov, M 2022, 'Elevated c-di-GMP Levels and Expression of the Type III Secretion System Promote Corneal Infection by Pseudomonas aeruginosa', Infection and Immunity, vol. 90, no. 8. https://doi.org/10.1128/iai.00061-22

APA

Yam, J. K. H., Aung, T. T., Chua, S. L., Cheng, Y., Kohli, G. S., Zhou, J., Constancias, F., Liu, Y., Cai, Z., Salido, M. M. S., Drautz-Moses, D. I., Rice, S. A., Schuster, S. C., Boo, Z. Z., Wu, B., Kjelleberg, S., Tolker-Nielsen, T., Lakshminarayanan, R., Beuerman, R. W., ... Givskov, M. (2022). Elevated c-di-GMP Levels and Expression of the Type III Secretion System Promote Corneal Infection by Pseudomonas aeruginosa. Infection and Immunity, 90(8). https://doi.org/10.1128/iai.00061-22

Vancouver

Yam JKH, Aung TT, Chua SL, Cheng Y, Kohli GS, Zhou J et al. Elevated c-di-GMP Levels and Expression of the Type III Secretion System Promote Corneal Infection by Pseudomonas aeruginosa. Infection and Immunity. 2022;90(8). https://doi.org/10.1128/iai.00061-22

Author

Yam, Joey Kuok Hoong ; Aung, Thet Tun ; Chua, Song Lin ; Cheng, Yingying ; Kohli, Gurjeet Singh ; Zhou, Jianuan ; Constancias, Florentin ; Liu, Yang ; Cai, Zhao ; Salido, May Margarette Santillan ; Drautz-Moses, Daniela I. ; Rice, Scott A. ; Schuster, Stephan Christoph ; Boo, Zhao Zhi ; Wu, Bin ; Kjelleberg, Staffan ; Tolker-Nielsen, Tim ; Lakshminarayanan, Rajamani ; Beuerman, Roger W. ; Yang, Liang ; Givskov, Michael. / Elevated c-di-GMP Levels and Expression of the Type III Secretion System Promote Corneal Infection by Pseudomonas aeruginosa. In: Infection and Immunity. 2022 ; Vol. 90, No. 8.

Bibtex

@article{95cd8c19f1e34dcd8100cc2573e00f6a,
title = "Elevated c-di-GMP Levels and Expression of the Type III Secretion System Promote Corneal Infection by Pseudomonas aeruginosa",
abstract = "Pseudomonas aeruginosa is generally believed to establish biofilm-associated infections under the regulation of the secondary messenger c-di-GMP. To evaluate P. aeruginosa biofilm physiology during ocular infections, comparative transcriptomic analysis was performed on wild-type P. aeruginosa PAO1, a DwspF mutant strain (high c-di-GMP levels), and a plac-yhjH-containing strain (low c-di-GMP levels) from mouse corneal infection, as well as in vitro biofilm and planktonic cultures. The c-di-GMP content in P. aeruginosa during corneal infection was monitored using a fluorescent c-di-GMP reporter strain. Biofilm-related genes were induced in in vivo PAO1 compared to in vitro planktonic bacteria. Several diguanylate cyclases and phosphodiesterases were commonly regulated in in vivo PAO1 and in vitro biofilm compared to in vitro planktonic bacteria. Several exopolysaccharide genes and motility genes were induced and downregulated, respectively, in in vivo PAO1 and the in vivo DwspF mutant compared to the in vivo plac-yhjH-containing strain. Elevation of c-di-GMP levels in P. aeruginosa began as early as 2 h postinfection. The DwspF mutant was less susceptible to host clearance than the plac-yhjH-containing strain and could suppress host immune responses. The type III secretion system (T3SS) was induced in in vivo PAO1 compared to in vitro biofilm bacteria. A DwspF mutant with a defective T3SS was more susceptible to host clearance than a DwspF mutant with a functional T3SS. Our study suggests that elevated intracellular c-di-GMP levels and T3SS activity in P. aeruginosa are necessary for establishment of infection and modulation of host immune responses in mouse cornea.",
keywords = "c-di-GMP, immune response, in vivo biofilms, mouse corneal infection, Pseudomonas aeruginosa, transcriptomics, type III secretion system",
author = "Yam, {Joey Kuok Hoong} and Aung, {Thet Tun} and Chua, {Song Lin} and Yingying Cheng and Kohli, {Gurjeet Singh} and Jianuan Zhou and Florentin Constancias and Yang Liu and Zhao Cai and Salido, {May Margarette Santillan} and Drautz-Moses, {Daniela I.} and Rice, {Scott A.} and Schuster, {Stephan Christoph} and Boo, {Zhao Zhi} and Bin Wu and Staffan Kjelleberg and Tim Tolker-Nielsen and Rajamani Lakshminarayanan and Beuerman, {Roger W.} and Liang Yang and Michael Givskov",
note = "Publisher Copyright: {\textcopyright} 2022 Yam et al.",
year = "2022",
doi = "10.1128/iai.00061-22",
language = "English",
volume = "90",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "8",

}

RIS

TY - JOUR

T1 - Elevated c-di-GMP Levels and Expression of the Type III Secretion System Promote Corneal Infection by Pseudomonas aeruginosa

AU - Yam, Joey Kuok Hoong

AU - Aung, Thet Tun

AU - Chua, Song Lin

AU - Cheng, Yingying

AU - Kohli, Gurjeet Singh

AU - Zhou, Jianuan

AU - Constancias, Florentin

AU - Liu, Yang

AU - Cai, Zhao

AU - Salido, May Margarette Santillan

AU - Drautz-Moses, Daniela I.

AU - Rice, Scott A.

AU - Schuster, Stephan Christoph

AU - Boo, Zhao Zhi

AU - Wu, Bin

AU - Kjelleberg, Staffan

AU - Tolker-Nielsen, Tim

AU - Lakshminarayanan, Rajamani

AU - Beuerman, Roger W.

AU - Yang, Liang

AU - Givskov, Michael

N1 - Publisher Copyright: © 2022 Yam et al.

PY - 2022

Y1 - 2022

N2 - Pseudomonas aeruginosa is generally believed to establish biofilm-associated infections under the regulation of the secondary messenger c-di-GMP. To evaluate P. aeruginosa biofilm physiology during ocular infections, comparative transcriptomic analysis was performed on wild-type P. aeruginosa PAO1, a DwspF mutant strain (high c-di-GMP levels), and a plac-yhjH-containing strain (low c-di-GMP levels) from mouse corneal infection, as well as in vitro biofilm and planktonic cultures. The c-di-GMP content in P. aeruginosa during corneal infection was monitored using a fluorescent c-di-GMP reporter strain. Biofilm-related genes were induced in in vivo PAO1 compared to in vitro planktonic bacteria. Several diguanylate cyclases and phosphodiesterases were commonly regulated in in vivo PAO1 and in vitro biofilm compared to in vitro planktonic bacteria. Several exopolysaccharide genes and motility genes were induced and downregulated, respectively, in in vivo PAO1 and the in vivo DwspF mutant compared to the in vivo plac-yhjH-containing strain. Elevation of c-di-GMP levels in P. aeruginosa began as early as 2 h postinfection. The DwspF mutant was less susceptible to host clearance than the plac-yhjH-containing strain and could suppress host immune responses. The type III secretion system (T3SS) was induced in in vivo PAO1 compared to in vitro biofilm bacteria. A DwspF mutant with a defective T3SS was more susceptible to host clearance than a DwspF mutant with a functional T3SS. Our study suggests that elevated intracellular c-di-GMP levels and T3SS activity in P. aeruginosa are necessary for establishment of infection and modulation of host immune responses in mouse cornea.

AB - Pseudomonas aeruginosa is generally believed to establish biofilm-associated infections under the regulation of the secondary messenger c-di-GMP. To evaluate P. aeruginosa biofilm physiology during ocular infections, comparative transcriptomic analysis was performed on wild-type P. aeruginosa PAO1, a DwspF mutant strain (high c-di-GMP levels), and a plac-yhjH-containing strain (low c-di-GMP levels) from mouse corneal infection, as well as in vitro biofilm and planktonic cultures. The c-di-GMP content in P. aeruginosa during corneal infection was monitored using a fluorescent c-di-GMP reporter strain. Biofilm-related genes were induced in in vivo PAO1 compared to in vitro planktonic bacteria. Several diguanylate cyclases and phosphodiesterases were commonly regulated in in vivo PAO1 and in vitro biofilm compared to in vitro planktonic bacteria. Several exopolysaccharide genes and motility genes were induced and downregulated, respectively, in in vivo PAO1 and the in vivo DwspF mutant compared to the in vivo plac-yhjH-containing strain. Elevation of c-di-GMP levels in P. aeruginosa began as early as 2 h postinfection. The DwspF mutant was less susceptible to host clearance than the plac-yhjH-containing strain and could suppress host immune responses. The type III secretion system (T3SS) was induced in in vivo PAO1 compared to in vitro biofilm bacteria. A DwspF mutant with a defective T3SS was more susceptible to host clearance than a DwspF mutant with a functional T3SS. Our study suggests that elevated intracellular c-di-GMP levels and T3SS activity in P. aeruginosa are necessary for establishment of infection and modulation of host immune responses in mouse cornea.

KW - c-di-GMP

KW - immune response

KW - in vivo biofilms

KW - mouse corneal infection

KW - Pseudomonas aeruginosa

KW - transcriptomics

KW - type III secretion system

U2 - 10.1128/iai.00061-22

DO - 10.1128/iai.00061-22

M3 - Journal article

C2 - 35913171

AN - SCOPUS:85136910897

VL - 90

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 8

ER -

ID: 320647332