Engineering PQS biosynthesis pathway for enhancement of bioelectricity production in Pseudomonas aeruginosa microbial fuel cells

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Engineering PQS biosynthesis pathway for enhancement of bioelectricity production in Pseudomonas aeruginosa microbial fuel cells. / Wang, Victor Bochuan; Chua, Song-Lin; Cao, Bin; Seviour, Thomas; Nesatyy, Victor J; Marsili, Enrico; Kjelleberg, Staffan; Givskov, Michael; Tolker-Nielsen, Tim; Song, Hao; Loo, Joachim Say Chye; Yang, Liang.

In: PLOS ONE, Vol. 8, No. 5, e63129, 2013, p. e63129.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Wang, VB, Chua, S-L, Cao, B, Seviour, T, Nesatyy, VJ, Marsili, E, Kjelleberg, S, Givskov, M, Tolker-Nielsen, T, Song, H, Loo, JSC & Yang, L 2013, 'Engineering PQS biosynthesis pathway for enhancement of bioelectricity production in Pseudomonas aeruginosa microbial fuel cells', PLOS ONE, vol. 8, no. 5, e63129, pp. e63129. https://doi.org/10.1371/journal.pone.0063129

APA

Wang, V. B., Chua, S-L., Cao, B., Seviour, T., Nesatyy, V. J., Marsili, E., Kjelleberg, S., Givskov, M., Tolker-Nielsen, T., Song, H., Loo, J. S. C., & Yang, L. (2013). Engineering PQS biosynthesis pathway for enhancement of bioelectricity production in Pseudomonas aeruginosa microbial fuel cells. PLOS ONE, 8(5), e63129. [e63129]. https://doi.org/10.1371/journal.pone.0063129

Vancouver

Wang VB, Chua S-L, Cao B, Seviour T, Nesatyy VJ, Marsili E et al. Engineering PQS biosynthesis pathway for enhancement of bioelectricity production in Pseudomonas aeruginosa microbial fuel cells. PLOS ONE. 2013;8(5):e63129. e63129. https://doi.org/10.1371/journal.pone.0063129

Author

Wang, Victor Bochuan ; Chua, Song-Lin ; Cao, Bin ; Seviour, Thomas ; Nesatyy, Victor J ; Marsili, Enrico ; Kjelleberg, Staffan ; Givskov, Michael ; Tolker-Nielsen, Tim ; Song, Hao ; Loo, Joachim Say Chye ; Yang, Liang. / Engineering PQS biosynthesis pathway for enhancement of bioelectricity production in Pseudomonas aeruginosa microbial fuel cells. In: PLOS ONE. 2013 ; Vol. 8, No. 5. pp. e63129.

Bibtex

@article{77fa51c464fa47d5a14f0305a4dade27,
title = "Engineering PQS biosynthesis pathway for enhancement of bioelectricity production in Pseudomonas aeruginosa microbial fuel cells",
abstract = "The biosynthesis of the redox shuttle, phenazines, in Pseudomonas aeruginosa, an ubiquitous microorganism in wastewater microflora, is regulated by the 2-heptyl-3,4-dihydroxyquinoline (PQS) quorum-sensing system. However, PQS inhibits anaerobic growth of P. aeruginosa. We constructed a P. aeruginosa strain that produces higher concentrations of phenazines under anaerobic conditions by over-expressing the PqsE effector in a PQS negative ΔpqsC mutant. The engineered strain exhibited an improved electrical performance in microbial fuel cells (MFCs) and potentiostat-controlled electrochemical cells with an approximate five-fold increase of maximum current density relative to the parent strain. Electrochemical analysis showed that the current increase correlates with an over-synthesis of phenazines. These results therefore demonstrate that targeting microbial cell-to-cell communication by genetic engineering is a suitable technique to improve power output of bioelectrochemical systems.",
keywords = "Bioelectric Energy Sources, Biofilms, Biosynthetic Pathways, Electricity, Genetic Engineering, Hydroxyquinolines, Phenazines, Pseudomonas aeruginosa, Pyocyanine, Quorum Sensing",
author = "Wang, {Victor Bochuan} and Song-Lin Chua and Bin Cao and Thomas Seviour and Nesatyy, {Victor J} and Enrico Marsili and Staffan Kjelleberg and Michael Givskov and Tim Tolker-Nielsen and Hao Song and Loo, {Joachim Say Chye} and Liang Yang",
year = "2013",
doi = "10.1371/journal.pone.0063129",
language = "English",
volume = "8",
pages = "e63129",
journal = "PLoS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "5",

}

RIS

TY - JOUR

T1 - Engineering PQS biosynthesis pathway for enhancement of bioelectricity production in Pseudomonas aeruginosa microbial fuel cells

AU - Wang, Victor Bochuan

AU - Chua, Song-Lin

AU - Cao, Bin

AU - Seviour, Thomas

AU - Nesatyy, Victor J

AU - Marsili, Enrico

AU - Kjelleberg, Staffan

AU - Givskov, Michael

AU - Tolker-Nielsen, Tim

AU - Song, Hao

AU - Loo, Joachim Say Chye

AU - Yang, Liang

PY - 2013

Y1 - 2013

N2 - The biosynthesis of the redox shuttle, phenazines, in Pseudomonas aeruginosa, an ubiquitous microorganism in wastewater microflora, is regulated by the 2-heptyl-3,4-dihydroxyquinoline (PQS) quorum-sensing system. However, PQS inhibits anaerobic growth of P. aeruginosa. We constructed a P. aeruginosa strain that produces higher concentrations of phenazines under anaerobic conditions by over-expressing the PqsE effector in a PQS negative ΔpqsC mutant. The engineered strain exhibited an improved electrical performance in microbial fuel cells (MFCs) and potentiostat-controlled electrochemical cells with an approximate five-fold increase of maximum current density relative to the parent strain. Electrochemical analysis showed that the current increase correlates with an over-synthesis of phenazines. These results therefore demonstrate that targeting microbial cell-to-cell communication by genetic engineering is a suitable technique to improve power output of bioelectrochemical systems.

AB - The biosynthesis of the redox shuttle, phenazines, in Pseudomonas aeruginosa, an ubiquitous microorganism in wastewater microflora, is regulated by the 2-heptyl-3,4-dihydroxyquinoline (PQS) quorum-sensing system. However, PQS inhibits anaerobic growth of P. aeruginosa. We constructed a P. aeruginosa strain that produces higher concentrations of phenazines under anaerobic conditions by over-expressing the PqsE effector in a PQS negative ΔpqsC mutant. The engineered strain exhibited an improved electrical performance in microbial fuel cells (MFCs) and potentiostat-controlled electrochemical cells with an approximate five-fold increase of maximum current density relative to the parent strain. Electrochemical analysis showed that the current increase correlates with an over-synthesis of phenazines. These results therefore demonstrate that targeting microbial cell-to-cell communication by genetic engineering is a suitable technique to improve power output of bioelectrochemical systems.

KW - Bioelectric Energy Sources

KW - Biofilms

KW - Biosynthetic Pathways

KW - Electricity

KW - Genetic Engineering

KW - Hydroxyquinolines

KW - Phenazines

KW - Pseudomonas aeruginosa

KW - Pyocyanine

KW - Quorum Sensing

U2 - 10.1371/journal.pone.0063129

DO - 10.1371/journal.pone.0063129

M3 - Journal article

C2 - 23700414

VL - 8

SP - e63129

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 5

M1 - e63129

ER -

ID: 95697683