TY - JOUR

T1 - Experimental Pseudomonas aeruginosa mediated rhino sinusitis in mink

AU - Kirkeby, S

AU - Hammer, A S

AU - Høiby, N

AU - Salomonsen, C.M.

N1 - Copyright © 2017 Elsevier Ireland Ltd. All rights reserved.

PY - 2017/5

Y1 - 2017/5

N2 - OBJECTIVES: The nasal and sinus cavities in children may serve as reservoirs for microorganisms that cause recurrent and chronic lung infections. This study evaluates whether the mink can be used as an animal model for studying Pseudomonas aeruginosa mediated rhino-sinusitis since there is no suitable traditional animal model for this disease.METHODS: Nasal tissue samples from infected and control mink were fixed in formalin, demineralized, and embedded in paraffin. A histological examination of sections from the infected animals revealed disintegration of the respiratory epithelium lining the nasal turbinates and swelling and edema of the submucosa. The expression of mucins and sialylated glycans was examined using immunohistochemistry.RESULTS: MUC1, MUC2 and MUC5AC were upregulated in the inoculated animals as a much stronger staining was present in the respiratory epithelium in the infected animals compared to the controls. The goblet cells in the nasal epithelium from the infected mink showed high affinity to the Maackia amurensis lectin and anti-asialo GM1 indicating a high concentration of α2-3 sialic acid respectively βGalNAc1-4Galβ containing glycans in these mucin producing cells. The nasal cavity in the infected mink shows features of carbohydrate expression comparable to what has been described in the respiratory system after Pseudomonas aeruginosa infection in humans.CONCLUSION: It is suggested that the mink is suitable for studying Pseudomonas aeruginosa mediated rhino-sinusitis.

AB - OBJECTIVES: The nasal and sinus cavities in children may serve as reservoirs for microorganisms that cause recurrent and chronic lung infections. This study evaluates whether the mink can be used as an animal model for studying Pseudomonas aeruginosa mediated rhino-sinusitis since there is no suitable traditional animal model for this disease.METHODS: Nasal tissue samples from infected and control mink were fixed in formalin, demineralized, and embedded in paraffin. A histological examination of sections from the infected animals revealed disintegration of the respiratory epithelium lining the nasal turbinates and swelling and edema of the submucosa. The expression of mucins and sialylated glycans was examined using immunohistochemistry.RESULTS: MUC1, MUC2 and MUC5AC were upregulated in the inoculated animals as a much stronger staining was present in the respiratory epithelium in the infected animals compared to the controls. The goblet cells in the nasal epithelium from the infected mink showed high affinity to the Maackia amurensis lectin and anti-asialo GM1 indicating a high concentration of α2-3 sialic acid respectively βGalNAc1-4Galβ containing glycans in these mucin producing cells. The nasal cavity in the infected mink shows features of carbohydrate expression comparable to what has been described in the respiratory system after Pseudomonas aeruginosa infection in humans.CONCLUSION: It is suggested that the mink is suitable for studying Pseudomonas aeruginosa mediated rhino-sinusitis.

KW - Animals

KW - Disease Models, Animal

KW - Immunohistochemistry

KW - Mink

KW - Mucins

KW - Nasal Mucosa

KW - Pseudomonas Infections

KW - Pseudomonas aeruginosa

KW - Rhinitis

KW - Sinusitis

KW - Journal Article

U2 - 10.1016/j.ijporl.2016.12.037

DO - 10.1016/j.ijporl.2016.12.037

M3 - Journal article

C2 - 28302328

VL - 96

SP - 156

EP - 163

JO - International Journal of Pediatric Otorhinolaryngology Extra

JF - International Journal of Pediatric Otorhinolaryngology Extra

SN - 1871-4048

ER -