Experimental reproducibility in flow-chamber biofilms

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Experimental reproducibility in flow-chamber biofilms. / Heydorn, Arne; Ersbøll, Bjarne Kjær; Hentzer, Morten; Parsek, M R; Givskov, M; Molin, Søren.

In: Microbiology, Vol. 146 ( Pt 10), 2000, p. 2409-15.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Heydorn, A, Ersbøll, BK, Hentzer, M, Parsek, MR, Givskov, M & Molin, S 2000, 'Experimental reproducibility in flow-chamber biofilms', Microbiology, vol. 146 ( Pt 10), pp. 2409-15.

APA

Heydorn, A., Ersbøll, B. K., Hentzer, M., Parsek, M. R., Givskov, M., & Molin, S. (2000). Experimental reproducibility in flow-chamber biofilms. Microbiology, 146 ( Pt 10), 2409-15.

Vancouver

Heydorn A, Ersbøll BK, Hentzer M, Parsek MR, Givskov M, Molin S. Experimental reproducibility in flow-chamber biofilms. Microbiology. 2000;146 ( Pt 10):2409-15.

Author

Heydorn, Arne ; Ersbøll, Bjarne Kjær ; Hentzer, Morten ; Parsek, M R ; Givskov, M ; Molin, Søren. / Experimental reproducibility in flow-chamber biofilms. In: Microbiology. 2000 ; Vol. 146 ( Pt 10). pp. 2409-15.

Bibtex

@article{70e5241f65e842d8bfd324b0bfd24147,
title = "Experimental reproducibility in flow-chamber biofilms",
abstract = "The structural organization of microbial communities is influenced by many factors, e.g. nutrient composition, shear stress and temperature. This paper presents a general method for quantitative comparison of biofilm structures and assessment of experimental reproducibility between independent biofilm experiments. By using a novel computer program, COMSTAT, biofilm structures of Pseudomonas aeruginosa and an isogenic rpoS mutant were quantified. The strains were tagged with the green fluorescent protein (GFP) and grown in flow chambers with a defined minimal medium as substrate. Three independent rounds of biofilm experiments were performed and in each round, each of the two variants was grown in two separate channels. Nine image stacks were acquired in each channel 146 h after inoculation. An analysis of variance model incorporating the factors experiment round, bacterial strain, channel number and image stack number was used to analyse the data calculated by COMSTAT. Experimental reproducibility was verified by estimating the magnitude of the variance of the effects round (sigma(2)R) and the interaction between bacterial strain and round (sigma(2)BR). Mean thickness of the wild-type and rpoS mutant biofilms was estimated at 6.31 microm (SE 0.81 microm) and 16.85 microm (SE 0.87 microm), respectively.",
author = "Arne Heydorn and Ersb{\o}ll, {Bjarne Kj{\ae}r} and Morten Hentzer and Parsek, {M R} and M Givskov and S{\o}ren Molin",
year = "2000",
language = "English",
volume = "146 ( Pt 10)",
pages = "2409--15",
journal = "Microbiology",
issn = "1350-0872",
publisher = "Society for General Microbiology",

}

RIS

TY - JOUR

T1 - Experimental reproducibility in flow-chamber biofilms

AU - Heydorn, Arne

AU - Ersbøll, Bjarne Kjær

AU - Hentzer, Morten

AU - Parsek, M R

AU - Givskov, M

AU - Molin, Søren

PY - 2000

Y1 - 2000

N2 - The structural organization of microbial communities is influenced by many factors, e.g. nutrient composition, shear stress and temperature. This paper presents a general method for quantitative comparison of biofilm structures and assessment of experimental reproducibility between independent biofilm experiments. By using a novel computer program, COMSTAT, biofilm structures of Pseudomonas aeruginosa and an isogenic rpoS mutant were quantified. The strains were tagged with the green fluorescent protein (GFP) and grown in flow chambers with a defined minimal medium as substrate. Three independent rounds of biofilm experiments were performed and in each round, each of the two variants was grown in two separate channels. Nine image stacks were acquired in each channel 146 h after inoculation. An analysis of variance model incorporating the factors experiment round, bacterial strain, channel number and image stack number was used to analyse the data calculated by COMSTAT. Experimental reproducibility was verified by estimating the magnitude of the variance of the effects round (sigma(2)R) and the interaction between bacterial strain and round (sigma(2)BR). Mean thickness of the wild-type and rpoS mutant biofilms was estimated at 6.31 microm (SE 0.81 microm) and 16.85 microm (SE 0.87 microm), respectively.

AB - The structural organization of microbial communities is influenced by many factors, e.g. nutrient composition, shear stress and temperature. This paper presents a general method for quantitative comparison of biofilm structures and assessment of experimental reproducibility between independent biofilm experiments. By using a novel computer program, COMSTAT, biofilm structures of Pseudomonas aeruginosa and an isogenic rpoS mutant were quantified. The strains were tagged with the green fluorescent protein (GFP) and grown in flow chambers with a defined minimal medium as substrate. Three independent rounds of biofilm experiments were performed and in each round, each of the two variants was grown in two separate channels. Nine image stacks were acquired in each channel 146 h after inoculation. An analysis of variance model incorporating the factors experiment round, bacterial strain, channel number and image stack number was used to analyse the data calculated by COMSTAT. Experimental reproducibility was verified by estimating the magnitude of the variance of the effects round (sigma(2)R) and the interaction between bacterial strain and round (sigma(2)BR). Mean thickness of the wild-type and rpoS mutant biofilms was estimated at 6.31 microm (SE 0.81 microm) and 16.85 microm (SE 0.87 microm), respectively.

M3 - Journal article

C2 - 11021917

VL - 146 ( Pt 10)

SP - 2409

EP - 2415

JO - Microbiology

JF - Microbiology

SN - 1350-0872

ER -

ID: 44305410