Gauging and visualizing c-di-GMP levels in pseudomonas aeruginosa using fluorescence-based biosensors

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Gauging and visualizing c-di-GMP levels in pseudomonas aeruginosa using fluorescence-based biosensors. / Rybtke, Morten; Chua, Song Lin; Yam, Joey Kuok Hoong; Givskov, Michael; Yang, Liang; Tolker-Nielsen, Tim.

c-di-GMP Signaling: Methods and Protocols. ed. / Karin Sauer. Vol. 1657 Humana Press, 2017. p. 87-98 (Methods in Molecular Biology, Vol. 1657).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Rybtke, M, Chua, SL, Yam, JKH, Givskov, M, Yang, L & Tolker-Nielsen, T 2017, Gauging and visualizing c-di-GMP levels in pseudomonas aeruginosa using fluorescence-based biosensors. in K Sauer (ed.), c-di-GMP Signaling: Methods and Protocols. vol. 1657, Humana Press, Methods in Molecular Biology, vol. 1657, pp. 87-98. https://doi.org/10.1007/978-1-4939-7240-1_8

APA

Rybtke, M., Chua, S. L., Yam, J. K. H., Givskov, M., Yang, L., & Tolker-Nielsen, T. (2017). Gauging and visualizing c-di-GMP levels in pseudomonas aeruginosa using fluorescence-based biosensors. In K. Sauer (Ed.), c-di-GMP Signaling: Methods and Protocols (Vol. 1657, pp. 87-98). Humana Press. Methods in Molecular Biology Vol. 1657 https://doi.org/10.1007/978-1-4939-7240-1_8

Vancouver

Rybtke M, Chua SL, Yam JKH, Givskov M, Yang L, Tolker-Nielsen T. Gauging and visualizing c-di-GMP levels in pseudomonas aeruginosa using fluorescence-based biosensors. In Sauer K, editor, c-di-GMP Signaling: Methods and Protocols. Vol. 1657. Humana Press. 2017. p. 87-98. (Methods in Molecular Biology, Vol. 1657). https://doi.org/10.1007/978-1-4939-7240-1_8

Author

Rybtke, Morten ; Chua, Song Lin ; Yam, Joey Kuok Hoong ; Givskov, Michael ; Yang, Liang ; Tolker-Nielsen, Tim. / Gauging and visualizing c-di-GMP levels in pseudomonas aeruginosa using fluorescence-based biosensors. c-di-GMP Signaling: Methods and Protocols. editor / Karin Sauer. Vol. 1657 Humana Press, 2017. pp. 87-98 (Methods in Molecular Biology, Vol. 1657).

Bibtex

@inbook{d415a9d4196643169a2aa765252f8736,
title = "Gauging and visualizing c-di-GMP levels in pseudomonas aeruginosa using fluorescence-based biosensors",
abstract = "Recent research has shown that the molecule c-di-GMP is an important second messenger regulating various functions in bacteria. In particular, the implication of c-di-GMP as a positive regulator of adhesion and biofilm formation has gained momentum as a highly relevant research topic, as detailed knowledge about the underlying regulatory mechanisms may enable the development of measures to control biofilms in both industrial and medical settings. Accordingly, it is in many cases of interest to measure the c-di-GMP level in bacteria under specific conditions or in specific mutant strains. We have developed a collection of fluorescence-based c-di-GMP biosensors capable of gauging the c-di-GMP level in Pseudomonas aeruginosa and closely related bacteria. Here, we describe protocols for the use of these biosensors in gauging and visualizing cellular c-di-GMP levels of P. aeruginosa both in in vitro setups such as continuous-culture flow-cell biofilms, and in in vivo settings such as a murine corneal infection model.",
keywords = "Biofilm, Biosensor, c-di-GMP, Cyclic di-GMP, Fluorescence, Ocular infection, Pseudomonas",
author = "Morten Rybtke and Chua, {Song Lin} and Yam, {Joey Kuok Hoong} and Michael Givskov and Liang Yang and Tim Tolker-Nielsen",
year = "2017",
month = jan,
doi = "10.1007/978-1-4939-7240-1_8",
language = "English",
isbn = "978-1-4939-7239-5",
volume = "1657",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "87--98",
editor = "Karin Sauer",
booktitle = "c-di-GMP Signaling",
address = "United States",

}

RIS

TY - CHAP

T1 - Gauging and visualizing c-di-GMP levels in pseudomonas aeruginosa using fluorescence-based biosensors

AU - Rybtke, Morten

AU - Chua, Song Lin

AU - Yam, Joey Kuok Hoong

AU - Givskov, Michael

AU - Yang, Liang

AU - Tolker-Nielsen, Tim

PY - 2017/1

Y1 - 2017/1

N2 - Recent research has shown that the molecule c-di-GMP is an important second messenger regulating various functions in bacteria. In particular, the implication of c-di-GMP as a positive regulator of adhesion and biofilm formation has gained momentum as a highly relevant research topic, as detailed knowledge about the underlying regulatory mechanisms may enable the development of measures to control biofilms in both industrial and medical settings. Accordingly, it is in many cases of interest to measure the c-di-GMP level in bacteria under specific conditions or in specific mutant strains. We have developed a collection of fluorescence-based c-di-GMP biosensors capable of gauging the c-di-GMP level in Pseudomonas aeruginosa and closely related bacteria. Here, we describe protocols for the use of these biosensors in gauging and visualizing cellular c-di-GMP levels of P. aeruginosa both in in vitro setups such as continuous-culture flow-cell biofilms, and in in vivo settings such as a murine corneal infection model.

AB - Recent research has shown that the molecule c-di-GMP is an important second messenger regulating various functions in bacteria. In particular, the implication of c-di-GMP as a positive regulator of adhesion and biofilm formation has gained momentum as a highly relevant research topic, as detailed knowledge about the underlying regulatory mechanisms may enable the development of measures to control biofilms in both industrial and medical settings. Accordingly, it is in many cases of interest to measure the c-di-GMP level in bacteria under specific conditions or in specific mutant strains. We have developed a collection of fluorescence-based c-di-GMP biosensors capable of gauging the c-di-GMP level in Pseudomonas aeruginosa and closely related bacteria. Here, we describe protocols for the use of these biosensors in gauging and visualizing cellular c-di-GMP levels of P. aeruginosa both in in vitro setups such as continuous-culture flow-cell biofilms, and in in vivo settings such as a murine corneal infection model.

KW - Biofilm

KW - Biosensor

KW - c-di-GMP

KW - Cyclic di-GMP

KW - Fluorescence

KW - Ocular infection

KW - Pseudomonas

U2 - 10.1007/978-1-4939-7240-1_8

DO - 10.1007/978-1-4939-7240-1_8

M3 - Book chapter

C2 - 28889288

AN - SCOPUS:85029390696

SN - 978-1-4939-7239-5

VL - 1657

T3 - Methods in Molecular Biology

SP - 87

EP - 98

BT - c-di-GMP Signaling

A2 - Sauer, Karin

PB - Humana Press

ER -

ID: 187265654