gfp-based N-acyl homoserine-lactone sensor systems for detection of bacterial communication

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

gfp-based N-acyl homoserine-lactone sensor systems for detection of bacterial communication. / Andersen, J B; Heydorn, Arne; Hentzer, Morten; Eberl, Leo; Geisenberger, O; Christensen, B B; Molin, Søren; Givskov, M.

In: Applied and Environmental Microbiology, Vol. 67, No. 2, 2001, p. 575-85.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Andersen, JB, Heydorn, A, Hentzer, M, Eberl, L, Geisenberger, O, Christensen, BB, Molin, S & Givskov, M 2001, 'gfp-based N-acyl homoserine-lactone sensor systems for detection of bacterial communication', Applied and Environmental Microbiology, vol. 67, no. 2, pp. 575-85. https://doi.org/10.1128/AEM.67.2.575-585.2001

APA

Andersen, J. B., Heydorn, A., Hentzer, M., Eberl, L., Geisenberger, O., Christensen, B. B., Molin, S., & Givskov, M. (2001). gfp-based N-acyl homoserine-lactone sensor systems for detection of bacterial communication. Applied and Environmental Microbiology, 67(2), 575-85. https://doi.org/10.1128/AEM.67.2.575-585.2001

Vancouver

Andersen JB, Heydorn A, Hentzer M, Eberl L, Geisenberger O, Christensen BB et al. gfp-based N-acyl homoserine-lactone sensor systems for detection of bacterial communication. Applied and Environmental Microbiology. 2001;67(2):575-85. https://doi.org/10.1128/AEM.67.2.575-585.2001

Author

Andersen, J B ; Heydorn, Arne ; Hentzer, Morten ; Eberl, Leo ; Geisenberger, O ; Christensen, B B ; Molin, Søren ; Givskov, M. / gfp-based N-acyl homoserine-lactone sensor systems for detection of bacterial communication. In: Applied and Environmental Microbiology. 2001 ; Vol. 67, No. 2. pp. 575-85.

Bibtex

@article{9fb5ae33ce18407ba49da5e7183eda00,
title = "gfp-based N-acyl homoserine-lactone sensor systems for detection of bacterial communication",
abstract = "In order to perform single-cell analysis and online studies of N-acyl homoserine lactone (AHL)-mediated communication among bacteria, components of the Vibrio fischeri quorum sensor encoded by luxR-P(luxI) have been fused to modified versions of gfpmut3* genes encoding unstable green fluorescent proteins. Bacterial strains harboring this green fluorescent sensor detected a broad spectrum of AHL molecules and were capable of sensing the presence of 5 nM N-3-oxohexanoyl-L-homoserine lactone in the surroundings. In combination with epifluorescent microscopy, the sensitivity of the sensor enabled AHL detection at the single-cell level and allowed for real-time measurements of fluctuations in AHL concentrations. This green fluorescent AHL sensor provides a state-of-the-art tool for studies of communication between the individuals present in mixed bacterial communities.",
author = "Andersen, {J B} and Arne Heydorn and Morten Hentzer and Leo Eberl and O Geisenberger and Christensen, {B B} and S{\o}ren Molin and M Givskov",
year = "2001",
doi = "10.1128/AEM.67.2.575-585.2001",
language = "English",
volume = "67",
pages = "575--85",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "2",

}

RIS

TY - JOUR

T1 - gfp-based N-acyl homoserine-lactone sensor systems for detection of bacterial communication

AU - Andersen, J B

AU - Heydorn, Arne

AU - Hentzer, Morten

AU - Eberl, Leo

AU - Geisenberger, O

AU - Christensen, B B

AU - Molin, Søren

AU - Givskov, M

PY - 2001

Y1 - 2001

N2 - In order to perform single-cell analysis and online studies of N-acyl homoserine lactone (AHL)-mediated communication among bacteria, components of the Vibrio fischeri quorum sensor encoded by luxR-P(luxI) have been fused to modified versions of gfpmut3* genes encoding unstable green fluorescent proteins. Bacterial strains harboring this green fluorescent sensor detected a broad spectrum of AHL molecules and were capable of sensing the presence of 5 nM N-3-oxohexanoyl-L-homoserine lactone in the surroundings. In combination with epifluorescent microscopy, the sensitivity of the sensor enabled AHL detection at the single-cell level and allowed for real-time measurements of fluctuations in AHL concentrations. This green fluorescent AHL sensor provides a state-of-the-art tool for studies of communication between the individuals present in mixed bacterial communities.

AB - In order to perform single-cell analysis and online studies of N-acyl homoserine lactone (AHL)-mediated communication among bacteria, components of the Vibrio fischeri quorum sensor encoded by luxR-P(luxI) have been fused to modified versions of gfpmut3* genes encoding unstable green fluorescent proteins. Bacterial strains harboring this green fluorescent sensor detected a broad spectrum of AHL molecules and were capable of sensing the presence of 5 nM N-3-oxohexanoyl-L-homoserine lactone in the surroundings. In combination with epifluorescent microscopy, the sensitivity of the sensor enabled AHL detection at the single-cell level and allowed for real-time measurements of fluctuations in AHL concentrations. This green fluorescent AHL sensor provides a state-of-the-art tool for studies of communication between the individuals present in mixed bacterial communities.

U2 - 10.1128/AEM.67.2.575-585.2001

DO - 10.1128/AEM.67.2.575-585.2001

M3 - Journal article

C2 - 11157219

VL - 67

SP - 575

EP - 585

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 2

ER -

ID: 44310609