Growing and analyzing biofilms in flow cells.

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Growing and analyzing biofilms in flow cells. / Sternberg, Claus; Tolker-Nielsen, Tim.

In: Current Protocols in Microbiology, Vol. Chapter 1, 2006, p. Unit 1B.2.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Sternberg, C & Tolker-Nielsen, T 2006, 'Growing and analyzing biofilms in flow cells.', Current Protocols in Microbiology, vol. Chapter 1, pp. Unit 1B.2. https://doi.org/10.1002/9780471729259.mc01b02s00

APA

Sternberg, C., & Tolker-Nielsen, T. (2006). Growing and analyzing biofilms in flow cells. Current Protocols in Microbiology, Chapter 1, Unit 1B.2. https://doi.org/10.1002/9780471729259.mc01b02s00

Vancouver

Sternberg C, Tolker-Nielsen T. Growing and analyzing biofilms in flow cells. Current Protocols in Microbiology. 2006;Chapter 1:Unit 1B.2. https://doi.org/10.1002/9780471729259.mc01b02s00

Author

Sternberg, Claus ; Tolker-Nielsen, Tim. / Growing and analyzing biofilms in flow cells. In: Current Protocols in Microbiology. 2006 ; Vol. Chapter 1. pp. Unit 1B.2.

Bibtex

@article{f0bf0830bc8011dd8e02000ea68e967b,
title = "Growing and analyzing biofilms in flow cells.",
abstract = "The setup of flow cell systems for the study of microbial biofilms and methods for the analysis of structural biofilm development are described in this unit. Use of flow cells allows direct microscopic investigation of biofilm development. The biofilms in flow cells develop under hydrodynamic conditions, and the environment can be carefully controlled and easily changed. The protocols in this unit include construction of the flow cell and the bubble trap, assembly and sterilization of the flow cell system, inoculation of the flow cells, running of the system, image capture and analysis, and disassembly and cleaning of the system. In addition, embedding and fluorescent in situ hybridization of flow cell-grown biofilms are addressed.",
author = "Claus Sternberg and Tim Tolker-Nielsen",
note = "Keywords: Biofilms; Bioreactors; Microscopy, Confocal",
year = "2006",
doi = "10.1002/9780471729259.mc01b02s00",
language = "English",
volume = "Chapter 1",
pages = "Unit 1B.2",
journal = "Current Protocols in Microbiology",
issn = "1934-8525",
publisher = "JohnWiley & Sons, Inc.",

}

RIS

TY - JOUR

T1 - Growing and analyzing biofilms in flow cells.

AU - Sternberg, Claus

AU - Tolker-Nielsen, Tim

N1 - Keywords: Biofilms; Bioreactors; Microscopy, Confocal

PY - 2006

Y1 - 2006

N2 - The setup of flow cell systems for the study of microbial biofilms and methods for the analysis of structural biofilm development are described in this unit. Use of flow cells allows direct microscopic investigation of biofilm development. The biofilms in flow cells develop under hydrodynamic conditions, and the environment can be carefully controlled and easily changed. The protocols in this unit include construction of the flow cell and the bubble trap, assembly and sterilization of the flow cell system, inoculation of the flow cells, running of the system, image capture and analysis, and disassembly and cleaning of the system. In addition, embedding and fluorescent in situ hybridization of flow cell-grown biofilms are addressed.

AB - The setup of flow cell systems for the study of microbial biofilms and methods for the analysis of structural biofilm development are described in this unit. Use of flow cells allows direct microscopic investigation of biofilm development. The biofilms in flow cells develop under hydrodynamic conditions, and the environment can be carefully controlled and easily changed. The protocols in this unit include construction of the flow cell and the bubble trap, assembly and sterilization of the flow cell system, inoculation of the flow cells, running of the system, image capture and analysis, and disassembly and cleaning of the system. In addition, embedding and fluorescent in situ hybridization of flow cell-grown biofilms are addressed.

U2 - 10.1002/9780471729259.mc01b02s00

DO - 10.1002/9780471729259.mc01b02s00

M3 - Journal article

C2 - 18770573

VL - Chapter 1

SP - Unit 1B.2

JO - Current Protocols in Microbiology

JF - Current Protocols in Microbiology

SN - 1934-8525

ER -

ID: 8777650