Growing and analyzing biofilms in flow chambers

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Growing and analyzing biofilms in flow chambers. / Tolker-Nielsen, Tim; Sternberg, Claus.

In: Current Protocols in Microbiology, Vol. 21, 2011, p. 1B.2.1-1B.2.17.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Tolker-Nielsen, T & Sternberg, C 2011, 'Growing and analyzing biofilms in flow chambers', Current Protocols in Microbiology, vol. 21, pp. 1B.2.1-1B.2.17. https://doi.org/10.1002/9780471729259.mc01b02s21

APA

Tolker-Nielsen, T., & Sternberg, C. (2011). Growing and analyzing biofilms in flow chambers. Current Protocols in Microbiology, 21, 1B.2.1-1B.2.17. https://doi.org/10.1002/9780471729259.mc01b02s21

Vancouver

Tolker-Nielsen T, Sternberg C. Growing and analyzing biofilms in flow chambers. Current Protocols in Microbiology. 2011;21:1B.2.1-1B.2.17. https://doi.org/10.1002/9780471729259.mc01b02s21

Author

Tolker-Nielsen, Tim ; Sternberg, Claus. / Growing and analyzing biofilms in flow chambers. In: Current Protocols in Microbiology. 2011 ; Vol. 21. pp. 1B.2.1-1B.2.17.

Bibtex

@article{cba8ba217eb24e7689843bbedcf133f4,
title = "Growing and analyzing biofilms in flow chambers",
abstract = "This unit describes the setup of flow chamber systems for the study of microbial biofilms, and methods for the analysis of structural biofilm formation. Use of flow chambers allows direct microscopic investigation of biofilm formation. The biofilms in flow chambers develop under hydrodynamic conditions, and the environment can be carefully controlled and easily changed. The protocols in this unit include construction of the flow chamber and the bubble trap, assembly and sterilization of the flow chamber system, inoculation of the flow chambers, running of the system, image capture and analysis, and disassembly and cleaning of the system. In addition, embedding and fluorescent in situ hybridization of flow chamber-grown biofilms are addressed.",
keywords = "Bacteria, Biofilms, Image Processing, Computer-Assisted, Microbiological Techniques, Microscopy, Confocal",
author = "Tim Tolker-Nielsen and Claus Sternberg",
year = "2011",
doi = "10.1002/9780471729259.mc01b02s21",
language = "English",
volume = "21",
pages = "1B.2.1--1B.2.17",
journal = "Current Protocols in Microbiology",
issn = "1934-8525",
publisher = "JohnWiley & Sons, Inc.",

}

RIS

TY - JOUR

T1 - Growing and analyzing biofilms in flow chambers

AU - Tolker-Nielsen, Tim

AU - Sternberg, Claus

PY - 2011

Y1 - 2011

N2 - This unit describes the setup of flow chamber systems for the study of microbial biofilms, and methods for the analysis of structural biofilm formation. Use of flow chambers allows direct microscopic investigation of biofilm formation. The biofilms in flow chambers develop under hydrodynamic conditions, and the environment can be carefully controlled and easily changed. The protocols in this unit include construction of the flow chamber and the bubble trap, assembly and sterilization of the flow chamber system, inoculation of the flow chambers, running of the system, image capture and analysis, and disassembly and cleaning of the system. In addition, embedding and fluorescent in situ hybridization of flow chamber-grown biofilms are addressed.

AB - This unit describes the setup of flow chamber systems for the study of microbial biofilms, and methods for the analysis of structural biofilm formation. Use of flow chambers allows direct microscopic investigation of biofilm formation. The biofilms in flow chambers develop under hydrodynamic conditions, and the environment can be carefully controlled and easily changed. The protocols in this unit include construction of the flow chamber and the bubble trap, assembly and sterilization of the flow chamber system, inoculation of the flow chambers, running of the system, image capture and analysis, and disassembly and cleaning of the system. In addition, embedding and fluorescent in situ hybridization of flow chamber-grown biofilms are addressed.

KW - Bacteria

KW - Biofilms

KW - Image Processing, Computer-Assisted

KW - Microbiological Techniques

KW - Microscopy, Confocal

U2 - 10.1002/9780471729259.mc01b02s21

DO - 10.1002/9780471729259.mc01b02s21

M3 - Journal article

C2 - 21538301

VL - 21

SP - 1B.2.1-1B.2.17

JO - Current Protocols in Microbiology

JF - Current Protocols in Microbiology

SN - 1934-8525

ER -

ID: 36061747