Identification of a new gene PA5017 involved in flagella-mediated motility, chemotaxis and biofilm formation in Pseudomonas aeruginosa.
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Identification of a new gene PA5017 involved in flagella-mediated motility, chemotaxis and biofilm formation in Pseudomonas aeruginosa. / Li, Yingli; Xia, Huiming; Bai, Fang; Xu, Haijin; Yang, Liang; Yao, Hongming; Zhang, Lu; Zhang, Xiuming; Bai, Yanling; Saris, Per E J; Tolker-Nielsen, Tim; Qiao, Mingqiang.
In: FEMS Microbiology Letters, Vol. 272, No. 2, 2007, p. 188-95.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Identification of a new gene PA5017 involved in flagella-mediated motility, chemotaxis and biofilm formation in Pseudomonas aeruginosa.
AU - Li, Yingli
AU - Xia, Huiming
AU - Bai, Fang
AU - Xu, Haijin
AU - Yang, Liang
AU - Yao, Hongming
AU - Zhang, Lu
AU - Zhang, Xiuming
AU - Bai, Yanling
AU - Saris, Per E J
AU - Tolker-Nielsen, Tim
AU - Qiao, Mingqiang
N1 - Keywords: Artificial Gene Fusion; Biofilms; Chemotaxis; DNA Transposable Elements; DNA, Bacterial; Flagella; Gene Deletion; Gene Expression Regulation, Bacterial; Genes, Bacterial; Genes, Reporter; Genetic Complementation Test; Locomotion; Microscopy, Electron, Scanning; Microscopy, Electron, Transmission; Molecular Sequence Data; Mutagenesis, Insertional; Operon; Pseudomonas aeruginosa; Sequence Analysis, DNA; beta-Galactosidase
PY - 2007
Y1 - 2007
N2 - Flagella-mediated motility is recognized as one of the major factors contributing to virulence in Pseudomonas aeruginosa. During a screening of a mini-Mu transposon mutant library of P. aeruginosa PA68, a mutant partially deficient in swimming and swarming motility was identified in a new locus that encodes a predicted protein of unknown function annotated PA5017 in the P. aeruginosa PAO1 genome sequence. Chemotaxis plate assay indicated that inactivation of the PA5017 gene led to a decreased chemotactic response. Complementation of the PA5017 mutant with the wild-type PA5017 gene restored normal motility and chemotaxis phenotype. A promoter-lacZ reporter activity assay of the cheYZAB operon from chemotaxis gene cluster 1 showed that there was almost a twofold difference in expression levels of the wild-type PA68 and the PA5017 mutant. This suggested that the PA5017 affected expression of the cheYZAB operon negatively. Further study showed that inactivation of the PA5017 gene in PA68 led to increased biofilm formation in a static system and to the formation of a heterogeneous biofilm in a flow-chamber system. These results suggested that PA5017 possibly affected flagellum-dependent motility and in turn biofilm formation via the chemotaxis signal transduction pathway.
AB - Flagella-mediated motility is recognized as one of the major factors contributing to virulence in Pseudomonas aeruginosa. During a screening of a mini-Mu transposon mutant library of P. aeruginosa PA68, a mutant partially deficient in swimming and swarming motility was identified in a new locus that encodes a predicted protein of unknown function annotated PA5017 in the P. aeruginosa PAO1 genome sequence. Chemotaxis plate assay indicated that inactivation of the PA5017 gene led to a decreased chemotactic response. Complementation of the PA5017 mutant with the wild-type PA5017 gene restored normal motility and chemotaxis phenotype. A promoter-lacZ reporter activity assay of the cheYZAB operon from chemotaxis gene cluster 1 showed that there was almost a twofold difference in expression levels of the wild-type PA68 and the PA5017 mutant. This suggested that the PA5017 affected expression of the cheYZAB operon negatively. Further study showed that inactivation of the PA5017 gene in PA68 led to increased biofilm formation in a static system and to the formation of a heterogeneous biofilm in a flow-chamber system. These results suggested that PA5017 possibly affected flagellum-dependent motility and in turn biofilm formation via the chemotaxis signal transduction pathway.
U2 - 10.1111/j.1574-6968.2007.00753.x
DO - 10.1111/j.1574-6968.2007.00753.x
M3 - Journal article
C2 - 17521365
VL - 272
SP - 188
EP - 195
JO - F E M S Microbiology Letters
JF - F E M S Microbiology Letters
SN - 0378-1097
IS - 2
ER -
ID: 8780119