Identification of Burkholderia cenocepacia strain H111 virulence factors using nonmammalian infection hosts

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Identification of Burkholderia cenocepacia strain H111 virulence factors using nonmammalian infection hosts. / Schwager, Stephan; Agnoli, Kirsty; Köthe, Manuela; Feldmann, Friederike; Givskov, Michael; Carlier, Aurelien; Eberl, Leo.

In: Infection and Immunity, Vol. 81, No. 1, 2013, p. 143-53.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Schwager, S, Agnoli, K, Köthe, M, Feldmann, F, Givskov, M, Carlier, A & Eberl, L 2013, 'Identification of Burkholderia cenocepacia strain H111 virulence factors using nonmammalian infection hosts', Infection and Immunity, vol. 81, no. 1, pp. 143-53. https://doi.org/10.1128/IAI.00768-12

APA

Schwager, S., Agnoli, K., Köthe, M., Feldmann, F., Givskov, M., Carlier, A., & Eberl, L. (2013). Identification of Burkholderia cenocepacia strain H111 virulence factors using nonmammalian infection hosts. Infection and Immunity, 81(1), 143-53. https://doi.org/10.1128/IAI.00768-12

Vancouver

Schwager S, Agnoli K, Köthe M, Feldmann F, Givskov M, Carlier A et al. Identification of Burkholderia cenocepacia strain H111 virulence factors using nonmammalian infection hosts. Infection and Immunity. 2013;81(1):143-53. https://doi.org/10.1128/IAI.00768-12

Author

Schwager, Stephan ; Agnoli, Kirsty ; Köthe, Manuela ; Feldmann, Friederike ; Givskov, Michael ; Carlier, Aurelien ; Eberl, Leo. / Identification of Burkholderia cenocepacia strain H111 virulence factors using nonmammalian infection hosts. In: Infection and Immunity. 2013 ; Vol. 81, No. 1. pp. 143-53.

Bibtex

@article{ea4005b36fd64097ab657327ad7e1711,
title = "Identification of Burkholderia cenocepacia strain H111 virulence factors using nonmammalian infection hosts",
abstract = "Burkholderia cenocepacia H111, a strain isolated from a cystic fibrosis patient, has been shown to effectively kill the nematode Caenorhabditis elegans. We used the C. elegans model of infection to screen a mini-Tn5 mutant library of B. cenocepacia H111 for attenuated virulence. Of the approximately 5,500 B. cenocepacia H111 random mini-Tn5 insertion mutants that were screened, 22 showed attenuated virulence in C. elegans. Except for the quorum-sensing regulator cepR, none of the mutated genes coded for the biosynthesis of classical virulence factors such as extracellular proteases or siderophores. Instead, the mutants contained insertions in metabolic and regulatory genes. Mutants attenuated in virulence in the C. elegans infection model were also tested in the Drosophila melanogaster pricking model, and those also attenuated in this model were further tested in Galleria mellonella. Six of the 22 mutants were attenuated in D. melanogaster, and five of these were less pathogenic in the G. mellonella model. We show that genes encoding enzymes of the purine, pyrimidine, and shikimate biosynthesis pathways are critical for virulence in multiple host models of infection.",
author = "Stephan Schwager and Kirsty Agnoli and Manuela K{\"o}the and Friederike Feldmann and Michael Givskov and Aurelien Carlier and Leo Eberl",
year = "2013",
doi = "10.1128/IAI.00768-12",
language = "English",
volume = "81",
pages = "143--53",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "1",

}

RIS

TY - JOUR

T1 - Identification of Burkholderia cenocepacia strain H111 virulence factors using nonmammalian infection hosts

AU - Schwager, Stephan

AU - Agnoli, Kirsty

AU - Köthe, Manuela

AU - Feldmann, Friederike

AU - Givskov, Michael

AU - Carlier, Aurelien

AU - Eberl, Leo

PY - 2013

Y1 - 2013

N2 - Burkholderia cenocepacia H111, a strain isolated from a cystic fibrosis patient, has been shown to effectively kill the nematode Caenorhabditis elegans. We used the C. elegans model of infection to screen a mini-Tn5 mutant library of B. cenocepacia H111 for attenuated virulence. Of the approximately 5,500 B. cenocepacia H111 random mini-Tn5 insertion mutants that were screened, 22 showed attenuated virulence in C. elegans. Except for the quorum-sensing regulator cepR, none of the mutated genes coded for the biosynthesis of classical virulence factors such as extracellular proteases or siderophores. Instead, the mutants contained insertions in metabolic and regulatory genes. Mutants attenuated in virulence in the C. elegans infection model were also tested in the Drosophila melanogaster pricking model, and those also attenuated in this model were further tested in Galleria mellonella. Six of the 22 mutants were attenuated in D. melanogaster, and five of these were less pathogenic in the G. mellonella model. We show that genes encoding enzymes of the purine, pyrimidine, and shikimate biosynthesis pathways are critical for virulence in multiple host models of infection.

AB - Burkholderia cenocepacia H111, a strain isolated from a cystic fibrosis patient, has been shown to effectively kill the nematode Caenorhabditis elegans. We used the C. elegans model of infection to screen a mini-Tn5 mutant library of B. cenocepacia H111 for attenuated virulence. Of the approximately 5,500 B. cenocepacia H111 random mini-Tn5 insertion mutants that were screened, 22 showed attenuated virulence in C. elegans. Except for the quorum-sensing regulator cepR, none of the mutated genes coded for the biosynthesis of classical virulence factors such as extracellular proteases or siderophores. Instead, the mutants contained insertions in metabolic and regulatory genes. Mutants attenuated in virulence in the C. elegans infection model were also tested in the Drosophila melanogaster pricking model, and those also attenuated in this model were further tested in Galleria mellonella. Six of the 22 mutants were attenuated in D. melanogaster, and five of these were less pathogenic in the G. mellonella model. We show that genes encoding enzymes of the purine, pyrimidine, and shikimate biosynthesis pathways are critical for virulence in multiple host models of infection.

U2 - 10.1128/IAI.00768-12

DO - 10.1128/IAI.00768-12

M3 - Journal article

C2 - 23090963

VL - 81

SP - 143

EP - 153

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 1

ER -

ID: 44311148