Immediate bromodeoxyuridine labelling of unseparated human bone marrow cells ex vivo is superior to labelling after routine laboratory processing

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Immediate bromodeoxyuridine labelling of unseparated human bone marrow cells ex vivo is superior to labelling after routine laboratory processing. / Jensen, P O; Mortensen, B T; Christensen, I J; Larsen, J K.

In: Cell Proliferation, Vol. 31, No. 1, 02.1998, p. 1-7.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Jensen, PO, Mortensen, BT, Christensen, IJ & Larsen, JK 1998, 'Immediate bromodeoxyuridine labelling of unseparated human bone marrow cells ex vivo is superior to labelling after routine laboratory processing', Cell Proliferation, vol. 31, no. 1, pp. 1-7.

APA

Jensen, P. O., Mortensen, B. T., Christensen, I. J., & Larsen, J. K. (1998). Immediate bromodeoxyuridine labelling of unseparated human bone marrow cells ex vivo is superior to labelling after routine laboratory processing. Cell Proliferation, 31(1), 1-7.

Vancouver

Jensen PO, Mortensen BT, Christensen IJ, Larsen JK. Immediate bromodeoxyuridine labelling of unseparated human bone marrow cells ex vivo is superior to labelling after routine laboratory processing. Cell Proliferation. 1998 Feb;31(1):1-7.

Author

Jensen, P O ; Mortensen, B T ; Christensen, I J ; Larsen, J K. / Immediate bromodeoxyuridine labelling of unseparated human bone marrow cells ex vivo is superior to labelling after routine laboratory processing. In: Cell Proliferation. 1998 ; Vol. 31, No. 1. pp. 1-7.

Bibtex

@article{09577cd651204fb5b8a56591f5133431,
title = "Immediate bromodeoxyuridine labelling of unseparated human bone marrow cells ex vivo is superior to labelling after routine laboratory processing",
abstract = "It is important to evaluate the proliferation of bone marrow cells in several disease conditions and during treatment of patients with for example cytokines. Labelling with bromodeoxyuridine (BrdUrd), immunocytochemical staining with anti-BrdUrd antibody and analysis by flow cytometry provides a reliable and reproducible technique for estimation of the fraction of cells that incorporated BrdUrd into DNA during S-phase. We have compared immediate BrdUrd labelling of unseparated bone marrow cells with the previously used labelling in the laboratory after routine separation of the mononuclear cells. Bone marrow aspirates from seven lymphoma patients without bone marrow involvement were studied with these two methods. We found higher BrdUrd labelling indices (LI) in the mononuclear cells, when cells were labelled immediately. A large variation in LI was found between patients. Our results suggest that ex vivo BrdUrd labelling of bone marrow cells should be performed immediately after aspiration and before separation, because these data are closer to values reported from in vivo labelling with BrdUrd.",
keywords = "Bone Marrow Cells, Bromodeoxyuridine, Cell Separation, Flow Cytometry, Humans, Journal Article, Research Support, Non-U.S. Gov't",
author = "Jensen, {P O} and Mortensen, {B T} and Christensen, {I J} and Larsen, {J K}",
year = "1998",
month = feb,
language = "English",
volume = "31",
pages = "1--7",
journal = "Cell Proliferation",
issn = "0960-7722",
publisher = "Wiley-Blackwell",
number = "1",

}

RIS

TY - JOUR

T1 - Immediate bromodeoxyuridine labelling of unseparated human bone marrow cells ex vivo is superior to labelling after routine laboratory processing

AU - Jensen, P O

AU - Mortensen, B T

AU - Christensen, I J

AU - Larsen, J K

PY - 1998/2

Y1 - 1998/2

N2 - It is important to evaluate the proliferation of bone marrow cells in several disease conditions and during treatment of patients with for example cytokines. Labelling with bromodeoxyuridine (BrdUrd), immunocytochemical staining with anti-BrdUrd antibody and analysis by flow cytometry provides a reliable and reproducible technique for estimation of the fraction of cells that incorporated BrdUrd into DNA during S-phase. We have compared immediate BrdUrd labelling of unseparated bone marrow cells with the previously used labelling in the laboratory after routine separation of the mononuclear cells. Bone marrow aspirates from seven lymphoma patients without bone marrow involvement were studied with these two methods. We found higher BrdUrd labelling indices (LI) in the mononuclear cells, when cells were labelled immediately. A large variation in LI was found between patients. Our results suggest that ex vivo BrdUrd labelling of bone marrow cells should be performed immediately after aspiration and before separation, because these data are closer to values reported from in vivo labelling with BrdUrd.

AB - It is important to evaluate the proliferation of bone marrow cells in several disease conditions and during treatment of patients with for example cytokines. Labelling with bromodeoxyuridine (BrdUrd), immunocytochemical staining with anti-BrdUrd antibody and analysis by flow cytometry provides a reliable and reproducible technique for estimation of the fraction of cells that incorporated BrdUrd into DNA during S-phase. We have compared immediate BrdUrd labelling of unseparated bone marrow cells with the previously used labelling in the laboratory after routine separation of the mononuclear cells. Bone marrow aspirates from seven lymphoma patients without bone marrow involvement were studied with these two methods. We found higher BrdUrd labelling indices (LI) in the mononuclear cells, when cells were labelled immediately. A large variation in LI was found between patients. Our results suggest that ex vivo BrdUrd labelling of bone marrow cells should be performed immediately after aspiration and before separation, because these data are closer to values reported from in vivo labelling with BrdUrd.

KW - Bone Marrow Cells

KW - Bromodeoxyuridine

KW - Cell Separation

KW - Flow Cytometry

KW - Humans

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - Journal article

C2 - 9666814

VL - 31

SP - 1

EP - 7

JO - Cell Proliferation

JF - Cell Proliferation

SN - 0960-7722

IS - 1

ER -

ID: 181873845