Inactivation of gltB abolishes expression of the assimilatory nitrate reductase gene (nasB) in Pseudomonas putida KT2442

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Inactivation of gltB abolishes expression of the assimilatory nitrate reductase gene (nasB) in Pseudomonas putida KT2442. / Eberl, Leo; Ammendola, A; Rothballer, M H; Givskov, M; Sternberg, C; Kilstrup, M; Schleifer, K H; Molin, Søren.

In: Journal of Bacteriology, Vol. 182, No. 12, 2000, p. 3368-76.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Eberl, L, Ammendola, A, Rothballer, MH, Givskov, M, Sternberg, C, Kilstrup, M, Schleifer, KH & Molin, S 2000, 'Inactivation of gltB abolishes expression of the assimilatory nitrate reductase gene (nasB) in Pseudomonas putida KT2442', Journal of Bacteriology, vol. 182, no. 12, pp. 3368-76.

APA

Eberl, L., Ammendola, A., Rothballer, M. H., Givskov, M., Sternberg, C., Kilstrup, M., Schleifer, K. H., & Molin, S. (2000). Inactivation of gltB abolishes expression of the assimilatory nitrate reductase gene (nasB) in Pseudomonas putida KT2442. Journal of Bacteriology, 182(12), 3368-76.

Vancouver

Eberl L, Ammendola A, Rothballer MH, Givskov M, Sternberg C, Kilstrup M et al. Inactivation of gltB abolishes expression of the assimilatory nitrate reductase gene (nasB) in Pseudomonas putida KT2442. Journal of Bacteriology. 2000;182(12):3368-76.

Author

Eberl, Leo ; Ammendola, A ; Rothballer, M H ; Givskov, M ; Sternberg, C ; Kilstrup, M ; Schleifer, K H ; Molin, Søren. / Inactivation of gltB abolishes expression of the assimilatory nitrate reductase gene (nasB) in Pseudomonas putida KT2442. In: Journal of Bacteriology. 2000 ; Vol. 182, No. 12. pp. 3368-76.

Bibtex

@article{299f3b76b660459191d2f7fccbb2b8b8,
title = "Inactivation of gltB abolishes expression of the assimilatory nitrate reductase gene (nasB) in Pseudomonas putida KT2442",
abstract = "By using mini-Tn5 transposon mutagenesis, random transcriptional fusions of promoterless bacterial luciferase, luxAB, to genes of Pseudomonas putida KT2442 were generated. Insertion mutants that responded to ammonium deficiency by induction of bioluminescence were selected. The mutant that responded most strongly was genetically analyzed and is demonstrated to bear the transposon within the assimilatory nitrate reductase gene (nasB) of P. putida KT2442. Genetic evidence as well as sequence analyses of the DNA regions flanking nasB suggest that the genes required for nitrate assimilation are not clustered. We isolated three second-site mutants in which induction of nasB expression was completely abolished under nitrogen-limiting conditions. Nucleotide sequence analysis of the chromosomal junctions revealed that in all three mutants the secondary transposon had inserted at different sites in the gltB gene of P. putida KT2442 encoding the major subunit of the glutamate synthase. A detailed physiological characterization of the gltB mutants revealed that they are unable to utilize a number of potential nitrogen sources, are defective in the ability to express nitrogen starvation proteins, display an aberrant cell morphology under nitrogen-limiting conditions, and are impaired in the capacity to survive prolonged nitrogen starvation periods.",
author = "Leo Eberl and A Ammendola and Rothballer, {M H} and M Givskov and C Sternberg and M Kilstrup and Schleifer, {K H} and S{\o}ren Molin",
year = "2000",
language = "English",
volume = "182",
pages = "3368--76",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "12",

}

RIS

TY - JOUR

T1 - Inactivation of gltB abolishes expression of the assimilatory nitrate reductase gene (nasB) in Pseudomonas putida KT2442

AU - Eberl, Leo

AU - Ammendola, A

AU - Rothballer, M H

AU - Givskov, M

AU - Sternberg, C

AU - Kilstrup, M

AU - Schleifer, K H

AU - Molin, Søren

PY - 2000

Y1 - 2000

N2 - By using mini-Tn5 transposon mutagenesis, random transcriptional fusions of promoterless bacterial luciferase, luxAB, to genes of Pseudomonas putida KT2442 were generated. Insertion mutants that responded to ammonium deficiency by induction of bioluminescence were selected. The mutant that responded most strongly was genetically analyzed and is demonstrated to bear the transposon within the assimilatory nitrate reductase gene (nasB) of P. putida KT2442. Genetic evidence as well as sequence analyses of the DNA regions flanking nasB suggest that the genes required for nitrate assimilation are not clustered. We isolated three second-site mutants in which induction of nasB expression was completely abolished under nitrogen-limiting conditions. Nucleotide sequence analysis of the chromosomal junctions revealed that in all three mutants the secondary transposon had inserted at different sites in the gltB gene of P. putida KT2442 encoding the major subunit of the glutamate synthase. A detailed physiological characterization of the gltB mutants revealed that they are unable to utilize a number of potential nitrogen sources, are defective in the ability to express nitrogen starvation proteins, display an aberrant cell morphology under nitrogen-limiting conditions, and are impaired in the capacity to survive prolonged nitrogen starvation periods.

AB - By using mini-Tn5 transposon mutagenesis, random transcriptional fusions of promoterless bacterial luciferase, luxAB, to genes of Pseudomonas putida KT2442 were generated. Insertion mutants that responded to ammonium deficiency by induction of bioluminescence were selected. The mutant that responded most strongly was genetically analyzed and is demonstrated to bear the transposon within the assimilatory nitrate reductase gene (nasB) of P. putida KT2442. Genetic evidence as well as sequence analyses of the DNA regions flanking nasB suggest that the genes required for nitrate assimilation are not clustered. We isolated three second-site mutants in which induction of nasB expression was completely abolished under nitrogen-limiting conditions. Nucleotide sequence analysis of the chromosomal junctions revealed that in all three mutants the secondary transposon had inserted at different sites in the gltB gene of P. putida KT2442 encoding the major subunit of the glutamate synthase. A detailed physiological characterization of the gltB mutants revealed that they are unable to utilize a number of potential nitrogen sources, are defective in the ability to express nitrogen starvation proteins, display an aberrant cell morphology under nitrogen-limiting conditions, and are impaired in the capacity to survive prolonged nitrogen starvation periods.

M3 - Journal article

C2 - 10852866

VL - 182

SP - 3368

EP - 3376

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 12

ER -

ID: 44305680