Induction of native c-di-GMP phosphodiesterases leads to dispersal of Pseudomonas aeruginosa biofilms

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Induction of native c-di-GMP phosphodiesterases leads to dispersal of Pseudomonas aeruginosa biofilms. / Andersen, Jens Bo; Kragh, Kasper Nørskov; Hultqvist, Louise Dahl; Rybtke, Morten; Nilsson, Martin; Jakobsen, Tim Holm; Givskov, Michael; Tolker-Nielsen, Tim.

In: Antimicrobial Agents and Chemotherapy, Vol. 65, No. 4, e02431-20, 2021.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Andersen, JB, Kragh, KN, Hultqvist, LD, Rybtke, M, Nilsson, M, Jakobsen, TH, Givskov, M & Tolker-Nielsen, T 2021, 'Induction of native c-di-GMP phosphodiesterases leads to dispersal of Pseudomonas aeruginosa biofilms', Antimicrobial Agents and Chemotherapy, vol. 65, no. 4, e02431-20. https://doi.org/10.1128/AAC.02431-20

APA

Andersen, J. B., Kragh, K. N., Hultqvist, L. D., Rybtke, M., Nilsson, M., Jakobsen, T. H., Givskov, M., & Tolker-Nielsen, T. (2021). Induction of native c-di-GMP phosphodiesterases leads to dispersal of Pseudomonas aeruginosa biofilms. Antimicrobial Agents and Chemotherapy, 65(4), [e02431-20]. https://doi.org/10.1128/AAC.02431-20

Vancouver

Andersen JB, Kragh KN, Hultqvist LD, Rybtke M, Nilsson M, Jakobsen TH et al. Induction of native c-di-GMP phosphodiesterases leads to dispersal of Pseudomonas aeruginosa biofilms. Antimicrobial Agents and Chemotherapy. 2021;65(4). e02431-20. https://doi.org/10.1128/AAC.02431-20

Author

Andersen, Jens Bo ; Kragh, Kasper Nørskov ; Hultqvist, Louise Dahl ; Rybtke, Morten ; Nilsson, Martin ; Jakobsen, Tim Holm ; Givskov, Michael ; Tolker-Nielsen, Tim. / Induction of native c-di-GMP phosphodiesterases leads to dispersal of Pseudomonas aeruginosa biofilms. In: Antimicrobial Agents and Chemotherapy. 2021 ; Vol. 65, No. 4.

Bibtex

@article{b497b49f635041909f52463caf675c48,
title = "Induction of native c-di-GMP phosphodiesterases leads to dispersal of Pseudomonas aeruginosa biofilms",
abstract = "A decade of research has shown that the molecule c-di-GMP functions as a central second messenger in many bacteria. A high level of c-di-GMP is associated with biofilm formation whereas a low level of c-di-GMP is associated with a planktonic single-cell bacterial lifestyle. C-di-GMP is formed by diguanylate cyclases and is degraded by specific phosphodiesterases. We have previously presented evidence that ectopic expression in Pseudomonas aeruginosa of the Escherichia coli phosphodiesterase YhjH results in biofilm dispersal. More recently, however, evidence has been presented that induction of native c-di-GMP phosphodiesterases does not lead to dispersal of P. aeruginosa biofilms. The latter result may discourage attempts to use c-di-GMP signaling as a target for development of anti-biofilm drugs. However, here we demonstrate that induction of the P. aeruginosa c-di-GMP phosphodiesterases PA2133 and BifA indeed does result in dispersal of P. aeruginosa biofilms in both a microtiter tray biofilm assay and in a flow-cell biofilm system.",
author = "Andersen, {Jens Bo} and Kragh, {Kasper N{\o}rskov} and Hultqvist, {Louise Dahl} and Morten Rybtke and Martin Nilsson and Jakobsen, {Tim Holm} and Michael Givskov and Tim Tolker-Nielsen",
note = "Copyright {\textcopyright} 2021 American Society for Microbiology.",
year = "2021",
doi = "10.1128/AAC.02431-20",
language = "English",
volume = "65",
journal = "Antimicrobial Agents and Chemotherapy",
issn = "0066-4804",
publisher = "American Society for Microbiology",
number = "4",

}

RIS

TY - JOUR

T1 - Induction of native c-di-GMP phosphodiesterases leads to dispersal of Pseudomonas aeruginosa biofilms

AU - Andersen, Jens Bo

AU - Kragh, Kasper Nørskov

AU - Hultqvist, Louise Dahl

AU - Rybtke, Morten

AU - Nilsson, Martin

AU - Jakobsen, Tim Holm

AU - Givskov, Michael

AU - Tolker-Nielsen, Tim

N1 - Copyright © 2021 American Society for Microbiology.

PY - 2021

Y1 - 2021

N2 - A decade of research has shown that the molecule c-di-GMP functions as a central second messenger in many bacteria. A high level of c-di-GMP is associated with biofilm formation whereas a low level of c-di-GMP is associated with a planktonic single-cell bacterial lifestyle. C-di-GMP is formed by diguanylate cyclases and is degraded by specific phosphodiesterases. We have previously presented evidence that ectopic expression in Pseudomonas aeruginosa of the Escherichia coli phosphodiesterase YhjH results in biofilm dispersal. More recently, however, evidence has been presented that induction of native c-di-GMP phosphodiesterases does not lead to dispersal of P. aeruginosa biofilms. The latter result may discourage attempts to use c-di-GMP signaling as a target for development of anti-biofilm drugs. However, here we demonstrate that induction of the P. aeruginosa c-di-GMP phosphodiesterases PA2133 and BifA indeed does result in dispersal of P. aeruginosa biofilms in both a microtiter tray biofilm assay and in a flow-cell biofilm system.

AB - A decade of research has shown that the molecule c-di-GMP functions as a central second messenger in many bacteria. A high level of c-di-GMP is associated with biofilm formation whereas a low level of c-di-GMP is associated with a planktonic single-cell bacterial lifestyle. C-di-GMP is formed by diguanylate cyclases and is degraded by specific phosphodiesterases. We have previously presented evidence that ectopic expression in Pseudomonas aeruginosa of the Escherichia coli phosphodiesterase YhjH results in biofilm dispersal. More recently, however, evidence has been presented that induction of native c-di-GMP phosphodiesterases does not lead to dispersal of P. aeruginosa biofilms. The latter result may discourage attempts to use c-di-GMP signaling as a target for development of anti-biofilm drugs. However, here we demonstrate that induction of the P. aeruginosa c-di-GMP phosphodiesterases PA2133 and BifA indeed does result in dispersal of P. aeruginosa biofilms in both a microtiter tray biofilm assay and in a flow-cell biofilm system.

U2 - 10.1128/AAC.02431-20

DO - 10.1128/AAC.02431-20

M3 - Journal article

C2 - 33495218

VL - 65

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

SN - 0066-4804

IS - 4

M1 - e02431-20

ER -

ID: 257705842