Induction of phospholipase- and flagellar synthesis in Serratia liquefaciens is controlled by expression of the flagellar master operon flhD

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Induction of phospholipase- and flagellar synthesis in Serratia liquefaciens is controlled by expression of the flagellar master operon flhD. / Givskov, M; Eberl, Leo; Christiansen, G; Benedik, M J; Molin, Søren.

In: Molecular Microbiology, Vol. 15, No. 3, 1995, p. 445-54.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Givskov, M, Eberl, L, Christiansen, G, Benedik, MJ & Molin, S 1995, 'Induction of phospholipase- and flagellar synthesis in Serratia liquefaciens is controlled by expression of the flagellar master operon flhD', Molecular Microbiology, vol. 15, no. 3, pp. 445-54.

APA

Givskov, M., Eberl, L., Christiansen, G., Benedik, M. J., & Molin, S. (1995). Induction of phospholipase- and flagellar synthesis in Serratia liquefaciens is controlled by expression of the flagellar master operon flhD. Molecular Microbiology, 15(3), 445-54.

Vancouver

Givskov M, Eberl L, Christiansen G, Benedik MJ, Molin S. Induction of phospholipase- and flagellar synthesis in Serratia liquefaciens is controlled by expression of the flagellar master operon flhD. Molecular Microbiology. 1995;15(3):445-54.

Author

Givskov, M ; Eberl, Leo ; Christiansen, G ; Benedik, M J ; Molin, Søren. / Induction of phospholipase- and flagellar synthesis in Serratia liquefaciens is controlled by expression of the flagellar master operon flhD. In: Molecular Microbiology. 1995 ; Vol. 15, No. 3. pp. 445-54.

Bibtex

@article{41dd241a9d3e4052b51811e149cfff87,
title = "Induction of phospholipase- and flagellar synthesis in Serratia liquefaciens is controlled by expression of the flagellar master operon flhD",
abstract = "When a liquid culture of Serratia spp. reaches the last part of the logarithmic phase of growth it induces the synthesis of several extracellular hydrolytic enzymes. In this communication we show that synthesis and secretion of the extracellular phospholipase is coupled to expression of flagella. Expression of flagella is demonstrated to follow a growth-phase-dependent pattern. Cloning, complementation studies and DNA-sequencing analysis has identified a genetic region in Serratia liquefaciens which exhibits extensive homology to the Escherichia coli flhD flagellar master operon. Interruption of the chromosomal flhD operon in S. liquefaciens results in non-flagellated and phospholipase-negative cells, but the synthesis of other exoenzymes is not affected. By placing the flhD operon under the control of a foreign inducible promoter we have shown that increased transcription through the flhD operon leads to induction of flagellar synthesis and phospholipase expression.",
author = "M Givskov and Leo Eberl and G Christiansen and Benedik, {M J} and S{\o}ren Molin",
year = "1995",
language = "English",
volume = "15",
pages = "445--54",
journal = "Molecular Microbiology",
issn = "0950-382X",
publisher = "Wiley-Blackwell",
number = "3",

}

RIS

TY - JOUR

T1 - Induction of phospholipase- and flagellar synthesis in Serratia liquefaciens is controlled by expression of the flagellar master operon flhD

AU - Givskov, M

AU - Eberl, Leo

AU - Christiansen, G

AU - Benedik, M J

AU - Molin, Søren

PY - 1995

Y1 - 1995

N2 - When a liquid culture of Serratia spp. reaches the last part of the logarithmic phase of growth it induces the synthesis of several extracellular hydrolytic enzymes. In this communication we show that synthesis and secretion of the extracellular phospholipase is coupled to expression of flagella. Expression of flagella is demonstrated to follow a growth-phase-dependent pattern. Cloning, complementation studies and DNA-sequencing analysis has identified a genetic region in Serratia liquefaciens which exhibits extensive homology to the Escherichia coli flhD flagellar master operon. Interruption of the chromosomal flhD operon in S. liquefaciens results in non-flagellated and phospholipase-negative cells, but the synthesis of other exoenzymes is not affected. By placing the flhD operon under the control of a foreign inducible promoter we have shown that increased transcription through the flhD operon leads to induction of flagellar synthesis and phospholipase expression.

AB - When a liquid culture of Serratia spp. reaches the last part of the logarithmic phase of growth it induces the synthesis of several extracellular hydrolytic enzymes. In this communication we show that synthesis and secretion of the extracellular phospholipase is coupled to expression of flagella. Expression of flagella is demonstrated to follow a growth-phase-dependent pattern. Cloning, complementation studies and DNA-sequencing analysis has identified a genetic region in Serratia liquefaciens which exhibits extensive homology to the Escherichia coli flhD flagellar master operon. Interruption of the chromosomal flhD operon in S. liquefaciens results in non-flagellated and phospholipase-negative cells, but the synthesis of other exoenzymes is not affected. By placing the flhD operon under the control of a foreign inducible promoter we have shown that increased transcription through the flhD operon leads to induction of flagellar synthesis and phospholipase expression.

M3 - Journal article

C2 - 7783615

VL - 15

SP - 445

EP - 454

JO - Molecular Microbiology

JF - Molecular Microbiology

SN - 0950-382X

IS - 3

ER -

ID: 44304770