Insight into the microbial multicellular lifestyle via flow-cell technology and confocal microscopy

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Insight into the microbial multicellular lifestyle via flow-cell technology and confocal microscopy. / Pamp, Sünje Johanna; Sternberg, Claus; Tolker-Nielsen, Tim.

In: Cytometry. Part A, Vol. 75, No. 2, 2009, p. 90-103.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Pamp, SJ, Sternberg, C & Tolker-Nielsen, T 2009, 'Insight into the microbial multicellular lifestyle via flow-cell technology and confocal microscopy', Cytometry. Part A, vol. 75, no. 2, pp. 90-103. https://doi.org/10.1002/cyto.a.20685

APA

Pamp, S. J., Sternberg, C., & Tolker-Nielsen, T. (2009). Insight into the microbial multicellular lifestyle via flow-cell technology and confocal microscopy. Cytometry. Part A, 75(2), 90-103. https://doi.org/10.1002/cyto.a.20685

Vancouver

Pamp SJ, Sternberg C, Tolker-Nielsen T. Insight into the microbial multicellular lifestyle via flow-cell technology and confocal microscopy. Cytometry. Part A. 2009;75(2):90-103. https://doi.org/10.1002/cyto.a.20685

Author

Pamp, Sünje Johanna ; Sternberg, Claus ; Tolker-Nielsen, Tim. / Insight into the microbial multicellular lifestyle via flow-cell technology and confocal microscopy. In: Cytometry. Part A. 2009 ; Vol. 75, No. 2. pp. 90-103.

Bibtex

@article{945727d07d3611df928f000ea68e967b,
title = "Insight into the microbial multicellular lifestyle via flow-cell technology and confocal microscopy",
abstract = "Biofilms are agglomerates of microorganisms surrounded by a self-produced extracellular matrix. During the last 10 years, there has been an increasing recognition of biofilms as a highly significant topic in microbiology with relevance for a variety of areas in our society including the environment, industry, and human health. Accordingly a number of biofilm model systems, molecular tools, microscopic techniques, and image analysis programs have been employed for the study of biofilms under controlled and reproducible conditions. Studies using confocal laser scanning microscopy (CLSM) of biofilms formed in flow-chamber experimental systems by genetically color-coded bacteria have provided detailed knowledge about biofilm developmental processes, cell differentiations, spatial organization, and function of laboratory-grown biofilms, in some cases down to the single cell level. In addition, the molecular mechanisms underlying the increased tolerance that biofilm cells often display towards antibiotic treatment are beginning to be unravelled.",
author = "Pamp, {S{\"u}nje Johanna} and Claus Sternberg and Tim Tolker-Nielsen",
note = "Keywords: Biofilms; Equipment Design; Flow Cytometry; Green Fluorescent Proteins; Microscopy, Confocal; Pseudomonas aeruginosa",
year = "2009",
doi = "10.1002/cyto.a.20685",
language = "English",
volume = "75",
pages = "90--103",
journal = "Cytometry. Supplement : the journal of the Society for Analytical Cytology",
issn = "1046-7386",
publisher = "JohnWiley & Sons, Inc.",
number = "2",

}

RIS

TY - JOUR

T1 - Insight into the microbial multicellular lifestyle via flow-cell technology and confocal microscopy

AU - Pamp, Sünje Johanna

AU - Sternberg, Claus

AU - Tolker-Nielsen, Tim

N1 - Keywords: Biofilms; Equipment Design; Flow Cytometry; Green Fluorescent Proteins; Microscopy, Confocal; Pseudomonas aeruginosa

PY - 2009

Y1 - 2009

N2 - Biofilms are agglomerates of microorganisms surrounded by a self-produced extracellular matrix. During the last 10 years, there has been an increasing recognition of biofilms as a highly significant topic in microbiology with relevance for a variety of areas in our society including the environment, industry, and human health. Accordingly a number of biofilm model systems, molecular tools, microscopic techniques, and image analysis programs have been employed for the study of biofilms under controlled and reproducible conditions. Studies using confocal laser scanning microscopy (CLSM) of biofilms formed in flow-chamber experimental systems by genetically color-coded bacteria have provided detailed knowledge about biofilm developmental processes, cell differentiations, spatial organization, and function of laboratory-grown biofilms, in some cases down to the single cell level. In addition, the molecular mechanisms underlying the increased tolerance that biofilm cells often display towards antibiotic treatment are beginning to be unravelled.

AB - Biofilms are agglomerates of microorganisms surrounded by a self-produced extracellular matrix. During the last 10 years, there has been an increasing recognition of biofilms as a highly significant topic in microbiology with relevance for a variety of areas in our society including the environment, industry, and human health. Accordingly a number of biofilm model systems, molecular tools, microscopic techniques, and image analysis programs have been employed for the study of biofilms under controlled and reproducible conditions. Studies using confocal laser scanning microscopy (CLSM) of biofilms formed in flow-chamber experimental systems by genetically color-coded bacteria have provided detailed knowledge about biofilm developmental processes, cell differentiations, spatial organization, and function of laboratory-grown biofilms, in some cases down to the single cell level. In addition, the molecular mechanisms underlying the increased tolerance that biofilm cells often display towards antibiotic treatment are beginning to be unravelled.

U2 - 10.1002/cyto.a.20685

DO - 10.1002/cyto.a.20685

M3 - Journal article

C2 - 19051241

VL - 75

SP - 90

EP - 103

JO - Cytometry. Supplement : the journal of the Society for Analytical Cytology

JF - Cytometry. Supplement : the journal of the Society for Analytical Cytology

SN - 1046-7386

IS - 2

ER -

ID: 20394326