Key Players and Individualists of Cyclic-di-GMP Signaling in Burkholderia cenocepacia

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Key Players and Individualists of Cyclic-di-GMP Signaling in Burkholderia cenocepacia. / Richter, Anja M; Fazli, Mustafa; Schmid, Nadine; Shilling, Rebecca; Suppiger, Angela; Givskov, Michael; Eberl, Leo; Tolker-Nielsen, Tim.

In: Frontiers in Microbiology, Vol. 9, 3286, 2019.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Richter, AM, Fazli, M, Schmid, N, Shilling, R, Suppiger, A, Givskov, M, Eberl, L & Tolker-Nielsen, T 2019, 'Key Players and Individualists of Cyclic-di-GMP Signaling in Burkholderia cenocepacia', Frontiers in Microbiology, vol. 9, 3286. https://doi.org/10.3389/fmicb.2018.03286

APA

Richter, A. M., Fazli, M., Schmid, N., Shilling, R., Suppiger, A., Givskov, M., Eberl, L., & Tolker-Nielsen, T. (2019). Key Players and Individualists of Cyclic-di-GMP Signaling in Burkholderia cenocepacia. Frontiers in Microbiology, 9, [3286]. https://doi.org/10.3389/fmicb.2018.03286

Vancouver

Richter AM, Fazli M, Schmid N, Shilling R, Suppiger A, Givskov M et al. Key Players and Individualists of Cyclic-di-GMP Signaling in Burkholderia cenocepacia. Frontiers in Microbiology. 2019;9. 3286. https://doi.org/10.3389/fmicb.2018.03286

Author

Richter, Anja M ; Fazli, Mustafa ; Schmid, Nadine ; Shilling, Rebecca ; Suppiger, Angela ; Givskov, Michael ; Eberl, Leo ; Tolker-Nielsen, Tim. / Key Players and Individualists of Cyclic-di-GMP Signaling in Burkholderia cenocepacia. In: Frontiers in Microbiology. 2019 ; Vol. 9.

Bibtex

@article{5e3a5276d2ca4d22a95d7fec6b41332b,
title = "Key Players and Individualists of Cyclic-di-GMP Signaling in Burkholderia cenocepacia",
abstract = "Burkholderia cenocepacia H111 is an opportunistic pathogen associated with chronic lung infections in cystic fibrosis patients. Biofilm formation, motility and virulence of B. cenocepacia are regulated by the second messenger cyclic di-guanosine monophosphate (c-di-GMP). In the present study, we analyzed the role of all 25 putative c-di-GMP metabolizing proteins of B. cenocepacia H111 with respect to motility, colony morphology, pellicle formation, biofilm formation, and virulence. We found that RpfR is a key regulator of c-di-GMP signaling in B. cenocepacia, affecting a broad spectrum of phenotypes under various environmental conditions. In addition, we identified Bcal2449 as a regulator of B. cenocepacia virulence in Galleria mellonella larvae. While Bcal2449 consists of protein domains that may catalyze both c-di-GMP synthesis and degradation, only the latter was essential for larvae killing, suggesting that a decreased c-di-GMP level mediated by the Bcal2449 protein is required for virulence of B. cenocepacia. Finally, our work suggests that some individual proteins play a role in regulating exclusively motility (CdpA), biofilm formation (Bcam1160) or both (Bcam2836).",
author = "Richter, {Anja M} and Mustafa Fazli and Nadine Schmid and Rebecca Shilling and Angela Suppiger and Michael Givskov and Leo Eberl and Tim Tolker-Nielsen",
year = "2019",
doi = "10.3389/fmicb.2018.03286",
language = "English",
volume = "9",
journal = "Frontiers in Microbiology",
issn = "1664-302X",
publisher = "Frontiers Media S.A.",

}

RIS

TY - JOUR

T1 - Key Players and Individualists of Cyclic-di-GMP Signaling in Burkholderia cenocepacia

AU - Richter, Anja M

AU - Fazli, Mustafa

AU - Schmid, Nadine

AU - Shilling, Rebecca

AU - Suppiger, Angela

AU - Givskov, Michael

AU - Eberl, Leo

AU - Tolker-Nielsen, Tim

PY - 2019

Y1 - 2019

N2 - Burkholderia cenocepacia H111 is an opportunistic pathogen associated with chronic lung infections in cystic fibrosis patients. Biofilm formation, motility and virulence of B. cenocepacia are regulated by the second messenger cyclic di-guanosine monophosphate (c-di-GMP). In the present study, we analyzed the role of all 25 putative c-di-GMP metabolizing proteins of B. cenocepacia H111 with respect to motility, colony morphology, pellicle formation, biofilm formation, and virulence. We found that RpfR is a key regulator of c-di-GMP signaling in B. cenocepacia, affecting a broad spectrum of phenotypes under various environmental conditions. In addition, we identified Bcal2449 as a regulator of B. cenocepacia virulence in Galleria mellonella larvae. While Bcal2449 consists of protein domains that may catalyze both c-di-GMP synthesis and degradation, only the latter was essential for larvae killing, suggesting that a decreased c-di-GMP level mediated by the Bcal2449 protein is required for virulence of B. cenocepacia. Finally, our work suggests that some individual proteins play a role in regulating exclusively motility (CdpA), biofilm formation (Bcam1160) or both (Bcam2836).

AB - Burkholderia cenocepacia H111 is an opportunistic pathogen associated with chronic lung infections in cystic fibrosis patients. Biofilm formation, motility and virulence of B. cenocepacia are regulated by the second messenger cyclic di-guanosine monophosphate (c-di-GMP). In the present study, we analyzed the role of all 25 putative c-di-GMP metabolizing proteins of B. cenocepacia H111 with respect to motility, colony morphology, pellicle formation, biofilm formation, and virulence. We found that RpfR is a key regulator of c-di-GMP signaling in B. cenocepacia, affecting a broad spectrum of phenotypes under various environmental conditions. In addition, we identified Bcal2449 as a regulator of B. cenocepacia virulence in Galleria mellonella larvae. While Bcal2449 consists of protein domains that may catalyze both c-di-GMP synthesis and degradation, only the latter was essential for larvae killing, suggesting that a decreased c-di-GMP level mediated by the Bcal2449 protein is required for virulence of B. cenocepacia. Finally, our work suggests that some individual proteins play a role in regulating exclusively motility (CdpA), biofilm formation (Bcam1160) or both (Bcam2836).

U2 - 10.3389/fmicb.2018.03286

DO - 10.3389/fmicb.2018.03286

M3 - Journal article

C2 - 30687272

VL - 9

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

M1 - 3286

ER -

ID: 216311344