Methods for studying biofilm formation: flow cells and confocal laser scanning microscopy

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Methods for studying biofilm formation : flow cells and confocal laser scanning microscopy. / Tolker-Nielsen, Tim; Sternberg, Claus.

In: Methods in Molecular Biology, Vol. 1149, 2014, p. 615-29.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Tolker-Nielsen, T & Sternberg, C 2014, 'Methods for studying biofilm formation: flow cells and confocal laser scanning microscopy', Methods in Molecular Biology, vol. 1149, pp. 615-29. https://doi.org/10.1007/978-1-4939-0473-0_47

APA

Tolker-Nielsen, T., & Sternberg, C. (2014). Methods for studying biofilm formation: flow cells and confocal laser scanning microscopy. Methods in Molecular Biology, 1149, 615-29. https://doi.org/10.1007/978-1-4939-0473-0_47

Vancouver

Tolker-Nielsen T, Sternberg C. Methods for studying biofilm formation: flow cells and confocal laser scanning microscopy. Methods in Molecular Biology. 2014;1149:615-29. https://doi.org/10.1007/978-1-4939-0473-0_47

Author

Tolker-Nielsen, Tim ; Sternberg, Claus. / Methods for studying biofilm formation : flow cells and confocal laser scanning microscopy. In: Methods in Molecular Biology. 2014 ; Vol. 1149. pp. 615-29.

Bibtex

@article{a073a1a8a8ee49288451687262780c5d,
title = "Methods for studying biofilm formation: flow cells and confocal laser scanning microscopy",
abstract = "In this chapter methods for growing and analyzing biofilms under hydrodynamic conditions in flow cells are described. Use of flow cells allows for direct microscopic investigation of biofilm formation. The flow in these chambers is essentially laminar, which means that the biofilms can be grown under highly controlled conditions, and that perturbations such as addition of antibiotics or change of the growth medium can be done efficiently at a defined time point. The protocol includes construction of the flow cell and the bubble trap, assembly and sterilization of the flow cell system, inoculation of the flow cells, running of the system, confocal laser scanning microscopy and image analysis, and disassembly and cleaning of the system.",
author = "Tim Tolker-Nielsen and Claus Sternberg",
year = "2014",
doi = "10.1007/978-1-4939-0473-0_47",
language = "English",
volume = "1149",
pages = "615--29",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
publisher = "Humana Press",

}

RIS

TY - JOUR

T1 - Methods for studying biofilm formation

T2 - flow cells and confocal laser scanning microscopy

AU - Tolker-Nielsen, Tim

AU - Sternberg, Claus

PY - 2014

Y1 - 2014

N2 - In this chapter methods for growing and analyzing biofilms under hydrodynamic conditions in flow cells are described. Use of flow cells allows for direct microscopic investigation of biofilm formation. The flow in these chambers is essentially laminar, which means that the biofilms can be grown under highly controlled conditions, and that perturbations such as addition of antibiotics or change of the growth medium can be done efficiently at a defined time point. The protocol includes construction of the flow cell and the bubble trap, assembly and sterilization of the flow cell system, inoculation of the flow cells, running of the system, confocal laser scanning microscopy and image analysis, and disassembly and cleaning of the system.

AB - In this chapter methods for growing and analyzing biofilms under hydrodynamic conditions in flow cells are described. Use of flow cells allows for direct microscopic investigation of biofilm formation. The flow in these chambers is essentially laminar, which means that the biofilms can be grown under highly controlled conditions, and that perturbations such as addition of antibiotics or change of the growth medium can be done efficiently at a defined time point. The protocol includes construction of the flow cell and the bubble trap, assembly and sterilization of the flow cell system, inoculation of the flow cells, running of the system, confocal laser scanning microscopy and image analysis, and disassembly and cleaning of the system.

U2 - 10.1007/978-1-4939-0473-0_47

DO - 10.1007/978-1-4939-0473-0_47

M3 - Journal article

C2 - 24818937

VL - 1149

SP - 615

EP - 629

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -

ID: 129018593