TY - JOUR

T1 - Microbiological aspects of phyllosoma rearing of the ornate rock lobster Panulirus ornatus

AU - Bourne, David

AU - Høj, Lone

AU - Webster, Nicole

AU - Payne, Matthew

AU - Skindersøe, Mette

AU - Givskov, Michael

AU - Hall, Mike

N1 - Funding Information: Matt Kenway, Don Booth, Matthew Salmon, Jane Gioffre, Grant Milton and Justin Hochen are thanked for the larval rearing of phyllosomas. Rick Webb, Centre of Microscopy and Microanalysis, University of Queensland is also thanked for assistance with SEM. The authors would also like to thank Neil Young for skilled preparation of phyllosomas for histopathology analysis, Jenny Swan for the technical help in the project and Tim Simmonds for the help in preparation of manuscript figures. Financial support for this research was received from AIMS, Jeff McCloy and the RLEAS Subprogram of the Fisheries Research and Development Corporation (FRDC).

PY - 2007/8/22

Y1 - 2007/8/22

N2 - Rock lobsters of the Palinuridae are the most valuable wild fisheries sector in Australia and are currently target aquaculture species. Significant challenges exist however to produce commercial scale quantities of post-larvae due to an extended larval phase which acerbates a high rate of larval attrition caused by inadequate nutrition and a challenging microbial environment. Here we investigate a diverse and varied bacterial community in four compartments of the larval-rearing system: the water column, the biofilm, live feeds and the phyllosomas themselves. External fouling of phyllosoma by filamentous Thiothrix sp. was documented by scanning electron microscopy (SEM) and fluorescence in situ hybridisation (FISH). Internal proliferation of bacteria coinciding with mass mortality of phyllosoma was observed in histopathological analysis and identified as Vibrio sp. by specific labelling of sectioned hepatopancreas tissue using FISH. Of particular interest in relation to larval mortalities was a range of Vibrio species, isolated from the four rearing compartments, closely affiliated with V. alginolyticus, V. parahaemolyticus, and V. harveyi. The presence of bacterial quorum sensing signal molecules within the system was demonstrated in both biofilm and phyllosoma environments during a larval-rearing run. Interestingly, a large increase in quorum sensing signal molecules was detected in phyllosoma corresponding with mass mortality.

AB - Rock lobsters of the Palinuridae are the most valuable wild fisheries sector in Australia and are currently target aquaculture species. Significant challenges exist however to produce commercial scale quantities of post-larvae due to an extended larval phase which acerbates a high rate of larval attrition caused by inadequate nutrition and a challenging microbial environment. Here we investigate a diverse and varied bacterial community in four compartments of the larval-rearing system: the water column, the biofilm, live feeds and the phyllosomas themselves. External fouling of phyllosoma by filamentous Thiothrix sp. was documented by scanning electron microscopy (SEM) and fluorescence in situ hybridisation (FISH). Internal proliferation of bacteria coinciding with mass mortality of phyllosoma was observed in histopathological analysis and identified as Vibrio sp. by specific labelling of sectioned hepatopancreas tissue using FISH. Of particular interest in relation to larval mortalities was a range of Vibrio species, isolated from the four rearing compartments, closely affiliated with V. alginolyticus, V. parahaemolyticus, and V. harveyi. The presence of bacterial quorum sensing signal molecules within the system was demonstrated in both biofilm and phyllosoma environments during a larval-rearing run. Interestingly, a large increase in quorum sensing signal molecules was detected in phyllosoma corresponding with mass mortality.

KW - Fluorescence in situ hybridisation

KW - Microbiology

KW - Panulirus ornatus

KW - Rock lobster

KW - Scanning electron microscopy

UR - http://www.scopus.com/inward/record.url?scp=34347351131&partnerID=8YFLogxK

U2 - 10.1016/j.aquaculture.2007.04.067

DO - 10.1016/j.aquaculture.2007.04.067

M3 - Journal article

AN - SCOPUS:34347351131

VL - 268

SP - 274

EP - 287

JO - Aquaculture

JF - Aquaculture

SN - 0044-8486

IS - 1-4 SPEC. ISS.

ER -