Mucoid Pseudomonas aeruginosa isolates maintain the biofilm formation capacity and the gene expression profiles during the chronic lung infection of CF patients
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Mucoid Pseudomonas aeruginosa isolates maintain the biofilm formation capacity and the gene expression profiles during the chronic lung infection of CF patients. / Lee, Baoleri; Schjerling, Charlotte K; Kirkby, Nikolai; Hoffmann, Nadine; Borup, Rehannah; Molin, Søren; Høiby, Niels; Ciofu, Oana.
In: Acta Pathologica Microbiologica et Immunologica Scandinavica, Vol. 119, No. 4-5, 2011, p. 263-74.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Mucoid Pseudomonas aeruginosa isolates maintain the biofilm formation capacity and the gene expression profiles during the chronic lung infection of CF patients
AU - Lee, Baoleri
AU - Schjerling, Charlotte K
AU - Kirkby, Nikolai
AU - Hoffmann, Nadine
AU - Borup, Rehannah
AU - Molin, Søren
AU - Høiby, Niels
AU - Ciofu, Oana
N1 - © 2011 The Authors. APMIS © 2011 APMIS.
PY - 2011
Y1 - 2011
N2 - Phenotypic and genotypic diversifications of Pseudomonas aeruginosa in the airways of patients with cystic fibrosis (CF) promote long-term survival of bacteria during chronic lung infection. Twelve clonally related, sequential mucoid and non-mucoid paired P. aeruginosa isolates obtained from three Danish CF patients were investigated. The in vitro biofilm formation capacity was studied under static and flow through conditions and the global gene expression profiles were investigated by Affymetrix GeneChip. Regulatory genes of alginate production and quorum sensing (QS) system were sequenced and measurements of the alginate production and the detection of the QS signal molecules were performed. Comparisons of mucoid and non-mucoid isolates from early and late stages of the infection showed that the mucoid phenotype maintained over a decade the capacity to form in vitro biofilm and showed an unaltered transcriptional profile, whereas substantial alterations in the transcriptional profiles and loss of the capacity to form in vitro biofilms were observed in corresponding isolates of the non-mucoid phenotype. The conserved gene expression pattern in the mucoid isolates vs the diversity of changes in non-mucoid isolates observed in this particular P. aeruginosa clone reflects different adaptation strategies used by these two phenotypes in the different niches of the CF lung environment.
AB - Phenotypic and genotypic diversifications of Pseudomonas aeruginosa in the airways of patients with cystic fibrosis (CF) promote long-term survival of bacteria during chronic lung infection. Twelve clonally related, sequential mucoid and non-mucoid paired P. aeruginosa isolates obtained from three Danish CF patients were investigated. The in vitro biofilm formation capacity was studied under static and flow through conditions and the global gene expression profiles were investigated by Affymetrix GeneChip. Regulatory genes of alginate production and quorum sensing (QS) system were sequenced and measurements of the alginate production and the detection of the QS signal molecules were performed. Comparisons of mucoid and non-mucoid isolates from early and late stages of the infection showed that the mucoid phenotype maintained over a decade the capacity to form in vitro biofilm and showed an unaltered transcriptional profile, whereas substantial alterations in the transcriptional profiles and loss of the capacity to form in vitro biofilms were observed in corresponding isolates of the non-mucoid phenotype. The conserved gene expression pattern in the mucoid isolates vs the diversity of changes in non-mucoid isolates observed in this particular P. aeruginosa clone reflects different adaptation strategies used by these two phenotypes in the different niches of the CF lung environment.
KW - Alginates
KW - Bacterial Proteins
KW - Biofilms
KW - Chronic Disease
KW - Cystic Fibrosis
KW - Denmark
KW - Gene Expression Profiling
KW - Genes, Bacterial
KW - Genotype
KW - Glucuronic Acid
KW - Hexuronic Acids
KW - Humans
KW - Lung Diseases
KW - Mutation
KW - Oligonucleotide Array Sequence Analysis
KW - Phenotype
KW - Pseudomonas Infections
KW - Pseudomonas aeruginosa
KW - Quorum Sensing
KW - Sigma Factor
KW - Trans-Activators
U2 - 10.1111/j.1600-0463.2011.02726.x
DO - 10.1111/j.1600-0463.2011.02726.x
M3 - Journal article
C2 - 21492226
VL - 119
SP - 263
EP - 274
JO - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica
JF - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica
SN - 0903-4641
IS - 4-5
ER -
ID: 33842899