Multiple roles of biosurfactants in structural biofilm development by Pseudomonas aeruginosa.

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Multiple roles of biosurfactants in structural biofilm development by Pseudomonas aeruginosa. / Pamp, Sünje Johanna; Tolker-Nielsen, Tim.

In: Journal of Bacteriology, Vol. 189, No. 6, 2007, p. 2531-9.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Pamp, SJ & Tolker-Nielsen, T 2007, 'Multiple roles of biosurfactants in structural biofilm development by Pseudomonas aeruginosa.', Journal of Bacteriology, vol. 189, no. 6, pp. 2531-9. https://doi.org/10.1128/JB.01515-06

APA

Pamp, S. J., & Tolker-Nielsen, T. (2007). Multiple roles of biosurfactants in structural biofilm development by Pseudomonas aeruginosa. Journal of Bacteriology, 189(6), 2531-9. https://doi.org/10.1128/JB.01515-06

Vancouver

Pamp SJ, Tolker-Nielsen T. Multiple roles of biosurfactants in structural biofilm development by Pseudomonas aeruginosa. Journal of Bacteriology. 2007;189(6):2531-9. https://doi.org/10.1128/JB.01515-06

Author

Pamp, Sünje Johanna ; Tolker-Nielsen, Tim. / Multiple roles of biosurfactants in structural biofilm development by Pseudomonas aeruginosa. In: Journal of Bacteriology. 2007 ; Vol. 189, No. 6. pp. 2531-9.

Bibtex

@article{d5d665d0bd3f11dd8e02000ea68e967b,
title = "Multiple roles of biosurfactants in structural biofilm development by Pseudomonas aeruginosa.",
abstract = "Recent studies have indicated that biosurfactants produced by Pseudomonas aeruginosa play a role both in maintaining channels between multicellular structures in biofilms and in dispersal of cells from biofilms. Through the use of flow cell technology and enhanced confocal laser scanning microscopy, we have obtained results which suggest that the biosurfactants produced by P. aeruginosa play additional roles in structural biofilm development. We present genetic evidence that during biofilm development by P. aeruginosa, biosurfactants promote microcolony formation in the initial phase and facilitate migration-dependent structural development in the later phase. P. aeruginosa rhlA mutants, deficient in synthesis of biosurfactants, were not capable of forming microcolonies in the initial phase of biofilm formation. Experiments involving two-color-coded mixed-strain biofilms showed that P. aeruginosa rhlA mutants were defective in migration-dependent development of mushroom-shaped multicellular structures in the later phase of biofilm formation. Experiments involving three-color-coded mixed-strain P. aeruginosa biofilms demonstrated that the wild-type and rhlA and pilA mutant strains formed distinct subpopulations on top of each other dependent on their ability to migrate and produce biosurfactants.",
author = "Pamp, {S{\"u}nje Johanna} and Tim Tolker-Nielsen",
note = "Keywords: Biofilms; Fimbriae Proteins; Glycolipids; Hexosyltransferases; Image Processing, Computer-Assisted; Microscopy, Confocal; Mutation; Pseudomonas aeruginosa; Surface-Active Agents",
year = "2007",
doi = "10.1128/JB.01515-06",
language = "English",
volume = "189",
pages = "2531--9",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "6",

}

RIS

TY - JOUR

T1 - Multiple roles of biosurfactants in structural biofilm development by Pseudomonas aeruginosa.

AU - Pamp, Sünje Johanna

AU - Tolker-Nielsen, Tim

N1 - Keywords: Biofilms; Fimbriae Proteins; Glycolipids; Hexosyltransferases; Image Processing, Computer-Assisted; Microscopy, Confocal; Mutation; Pseudomonas aeruginosa; Surface-Active Agents

PY - 2007

Y1 - 2007

N2 - Recent studies have indicated that biosurfactants produced by Pseudomonas aeruginosa play a role both in maintaining channels between multicellular structures in biofilms and in dispersal of cells from biofilms. Through the use of flow cell technology and enhanced confocal laser scanning microscopy, we have obtained results which suggest that the biosurfactants produced by P. aeruginosa play additional roles in structural biofilm development. We present genetic evidence that during biofilm development by P. aeruginosa, biosurfactants promote microcolony formation in the initial phase and facilitate migration-dependent structural development in the later phase. P. aeruginosa rhlA mutants, deficient in synthesis of biosurfactants, were not capable of forming microcolonies in the initial phase of biofilm formation. Experiments involving two-color-coded mixed-strain biofilms showed that P. aeruginosa rhlA mutants were defective in migration-dependent development of mushroom-shaped multicellular structures in the later phase of biofilm formation. Experiments involving three-color-coded mixed-strain P. aeruginosa biofilms demonstrated that the wild-type and rhlA and pilA mutant strains formed distinct subpopulations on top of each other dependent on their ability to migrate and produce biosurfactants.

AB - Recent studies have indicated that biosurfactants produced by Pseudomonas aeruginosa play a role both in maintaining channels between multicellular structures in biofilms and in dispersal of cells from biofilms. Through the use of flow cell technology and enhanced confocal laser scanning microscopy, we have obtained results which suggest that the biosurfactants produced by P. aeruginosa play additional roles in structural biofilm development. We present genetic evidence that during biofilm development by P. aeruginosa, biosurfactants promote microcolony formation in the initial phase and facilitate migration-dependent structural development in the later phase. P. aeruginosa rhlA mutants, deficient in synthesis of biosurfactants, were not capable of forming microcolonies in the initial phase of biofilm formation. Experiments involving two-color-coded mixed-strain biofilms showed that P. aeruginosa rhlA mutants were defective in migration-dependent development of mushroom-shaped multicellular structures in the later phase of biofilm formation. Experiments involving three-color-coded mixed-strain P. aeruginosa biofilms demonstrated that the wild-type and rhlA and pilA mutant strains formed distinct subpopulations on top of each other dependent on their ability to migrate and produce biosurfactants.

U2 - 10.1128/JB.01515-06

DO - 10.1128/JB.01515-06

M3 - Journal article

C2 - 17220224

VL - 189

SP - 2531

EP - 2539

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 6

ER -

ID: 8780175