N-acylhomoserine-lactone-mediated communication between Pseudomonas aeruginosa and Burkholderia cepacia in mixed biofilms
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N-acylhomoserine-lactone-mediated communication between Pseudomonas aeruginosa and Burkholderia cepacia in mixed biofilms. / Riedel, K; Hentzer, Morten; Geisenberger, O; Huber, B; Steidle, A; Wu, H; Høiby, N; Givskov, M; Molin, Søren; Eberl, Leo.
In: Microbiology, Vol. 147, No. Pt 12, 2001, p. 3249-62.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - N-acylhomoserine-lactone-mediated communication between Pseudomonas aeruginosa and Burkholderia cepacia in mixed biofilms
AU - Riedel, K
AU - Hentzer, Morten
AU - Geisenberger, O
AU - Huber, B
AU - Steidle, A
AU - Wu, H
AU - Høiby, N
AU - Givskov, M
AU - Molin, Søren
AU - Eberl, Leo
PY - 2001
Y1 - 2001
N2 - Pseudomonas aeruginosa and Burkholderia cepacia are capable of forming mixed biofilms in the lungs of cystic fibrosis patients. Both bacteria employ quorum-sensing systems, which rely on N-acylhomoserine lactone (AHL) signal molecules, to co-ordinate expression of virulence factors with the formation of biofilms. As both bacteria utilize the same class of signal molecules the authors investigated whether communication between the species occurs. To address this issue, novel Gfp-based biosensors for non-destructive, in situ detection of AHLs were constructed and characterized. These sensors were used to visualize AHL-mediated communication in mixed biofilms, which were cultivated either in artificial flow chambers or in alginate beads in mouse lung tissue. In both model systems B. cepacia was capable of perceiving the AHL signals produced by P. aeruginosa, while the latter strain did not respond to the molecules produced by B. cepacia. Measurements of extracellular proteolytic activities of defined quorum-sensing mutants grown in media complemented with AHL extracts prepared from culture supernatants of various wild-type and mutant strains supported the view of unidirectional signalling between the two strains.
AB - Pseudomonas aeruginosa and Burkholderia cepacia are capable of forming mixed biofilms in the lungs of cystic fibrosis patients. Both bacteria employ quorum-sensing systems, which rely on N-acylhomoserine lactone (AHL) signal molecules, to co-ordinate expression of virulence factors with the formation of biofilms. As both bacteria utilize the same class of signal molecules the authors investigated whether communication between the species occurs. To address this issue, novel Gfp-based biosensors for non-destructive, in situ detection of AHLs were constructed and characterized. These sensors were used to visualize AHL-mediated communication in mixed biofilms, which were cultivated either in artificial flow chambers or in alginate beads in mouse lung tissue. In both model systems B. cepacia was capable of perceiving the AHL signals produced by P. aeruginosa, while the latter strain did not respond to the molecules produced by B. cepacia. Measurements of extracellular proteolytic activities of defined quorum-sensing mutants grown in media complemented with AHL extracts prepared from culture supernatants of various wild-type and mutant strains supported the view of unidirectional signalling between the two strains.
KW - Animals
KW - Biofilms
KW - Burkholderia Infections
KW - Burkholderia cepacia
KW - Endopeptidases
KW - Genes, Reporter
KW - Green Fluorescent Proteins
KW - Homoserine
KW - Luminescent Proteins
KW - Lung Diseases
KW - Mice
KW - Pheromones
KW - Pseudomonas Infections
KW - Pseudomonas aeruginosa
KW - Signal Transduction
M3 - Journal article
C2 - 11739757
VL - 147
SP - 3249
EP - 3262
JO - Microbiology
JF - Microbiology
SN - 1350-0872
IS - Pt 12
ER -
ID: 44310300