Novel human in vitro vegetation simulation model for infective endocarditis
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Novel human in vitro vegetation simulation model for infective endocarditis. / Schwartz, Franziska A.; Christophersen, Lars; Laulund, Anne Sofie; Lundquist, Rasmus; Lerche, Christian; Rude Nielsen, Pia; Bundgaard, Henning; Høiby, Niels; Moser, Claus.
In: APMIS, Vol. 129, No. 11, 2021, p. 653-662.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Novel human in vitro vegetation simulation model for infective endocarditis
AU - Schwartz, Franziska A.
AU - Christophersen, Lars
AU - Laulund, Anne Sofie
AU - Lundquist, Rasmus
AU - Lerche, Christian
AU - Rude Nielsen, Pia
AU - Bundgaard, Henning
AU - Høiby, Niels
AU - Moser, Claus
N1 - Publisher Copyright: © 2021 Scandinavian Societies for Medical Microbiology and Pathology.
PY - 2021
Y1 - 2021
N2 - Infective endocarditis (IE) is a heart valve infection with high mortality rates. IE results from epithelial lesions, inducing sterile healing vegetations consisting of platelets, leucocytes, and fibrin that are susceptible for colonization by temporary bacteremia. Clinical testing of new treatments for IE is difficult and fast models sparse. The present study aimed at establishing an in vitro vegetation simulation IE model for fast screening of novel treatment strategies. A healing promoting platelet and leucocyte-rich fibrin patch was used to establish an IE organoid-like model by colonization with IE-associated bacterial isolates Staphylococcus aureus, Streptococcus spp (S. mitis group), and Enterococcus faecalis. The patch was subsequently exposed to tobramycin, ciprofloxacin, or penicillin. Bacterial colonization was evaluated by microscopy and quantitative bacteriology. We achieved stable bacterial colonization on the patch, comparable to clinical IE vegetations. Microscopy revealed uneven, biofilm-like colonization of the patch. The surface-associated bacteria displayed increased tolerance to antibiotics compared to planktonic bacteria. The present study succeeded in establishing an IE simulation model with the relevant pathogens S. aureus, S. mitis group, and E. faecalis. The findings indicate that the IE model mirrors the natural IE process and has the potential for fast screening of treatment candidates.
AB - Infective endocarditis (IE) is a heart valve infection with high mortality rates. IE results from epithelial lesions, inducing sterile healing vegetations consisting of platelets, leucocytes, and fibrin that are susceptible for colonization by temporary bacteremia. Clinical testing of new treatments for IE is difficult and fast models sparse. The present study aimed at establishing an in vitro vegetation simulation IE model for fast screening of novel treatment strategies. A healing promoting platelet and leucocyte-rich fibrin patch was used to establish an IE organoid-like model by colonization with IE-associated bacterial isolates Staphylococcus aureus, Streptococcus spp (S. mitis group), and Enterococcus faecalis. The patch was subsequently exposed to tobramycin, ciprofloxacin, or penicillin. Bacterial colonization was evaluated by microscopy and quantitative bacteriology. We achieved stable bacterial colonization on the patch, comparable to clinical IE vegetations. Microscopy revealed uneven, biofilm-like colonization of the patch. The surface-associated bacteria displayed increased tolerance to antibiotics compared to planktonic bacteria. The present study succeeded in establishing an IE simulation model with the relevant pathogens S. aureus, S. mitis group, and E. faecalis. The findings indicate that the IE model mirrors the natural IE process and has the potential for fast screening of treatment candidates.
KW - antibiotic tolerance
KW - Biofilm
KW - histopathology
KW - host response
KW - infective endocarditis
KW - organoid-like simulation
U2 - 10.1111/apm.13182
DO - 10.1111/apm.13182
M3 - Journal article
C2 - 34580927
AN - SCOPUS:85117200193
VL - 129
SP - 653
EP - 662
JO - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica
JF - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica
SN - 0903-4641
IS - 11
ER -
ID: 283138951