Phenotypes selected during chronic lung infection in cystic fibrosis patients: implications for the treatment of Pseudomonas aeruginosa biofilm infections
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Phenotypes selected during chronic lung infection in cystic fibrosis patients : implications for the treatment of Pseudomonas aeruginosa biofilm infections. / Ciofu, Oana; Mandsberg, Lotte F; Wang, Hengzhuang; Høiby, Niels.
In: F E M S Immunology and Medical Microbiology, Vol. 65, No. 2, 07.2012, p. 215-225.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Phenotypes selected during chronic lung infection in cystic fibrosis patients
T2 - implications for the treatment of Pseudomonas aeruginosa biofilm infections
AU - Ciofu, Oana
AU - Mandsberg, Lotte F
AU - Wang, Hengzhuang
AU - Høiby, Niels
N1 - © 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
PY - 2012/7
Y1 - 2012/7
N2 - During chronic lung infection of patients with cystic fibrosis, Pseudomonas aeruginosa can survive for long periods of time under the challenging selective pressure imposed by the immune system and antibiotic treatment as a result of its biofilm mode of growth and adaptive evolution mediated by genetic variation. Mucoidy, hypermutability and acquirement of mutational antibiotic resistance are important adaptive phenotypes that are selected during chronic P. aeruginosa infection. This review dicsusses the role played by these phenotypes for the tolerance of biofilms to antibiotics and show that mucoidy and hypermutability change the architecture of in vitro formed biofilms and lead to increase tolerance to antibiotics. Production of high levels of beta-lactamase impairs penetration of beta-lactam antibiotics due to inactivation of the antibiotic. In conclusion, these data underline the importance of biofilm prevention strategies by early aggressive antibiotic prophylaxis or therapy before phenotypic diversification during chronic lung infection of patients with cystic fibrosis.
AB - During chronic lung infection of patients with cystic fibrosis, Pseudomonas aeruginosa can survive for long periods of time under the challenging selective pressure imposed by the immune system and antibiotic treatment as a result of its biofilm mode of growth and adaptive evolution mediated by genetic variation. Mucoidy, hypermutability and acquirement of mutational antibiotic resistance are important adaptive phenotypes that are selected during chronic P. aeruginosa infection. This review dicsusses the role played by these phenotypes for the tolerance of biofilms to antibiotics and show that mucoidy and hypermutability change the architecture of in vitro formed biofilms and lead to increase tolerance to antibiotics. Production of high levels of beta-lactamase impairs penetration of beta-lactam antibiotics due to inactivation of the antibiotic. In conclusion, these data underline the importance of biofilm prevention strategies by early aggressive antibiotic prophylaxis or therapy before phenotypic diversification during chronic lung infection of patients with cystic fibrosis.
KW - Anti-Bacterial Agents
KW - Biofilms
KW - Chronic Disease
KW - Cystic Fibrosis
KW - Humans
KW - Lung
KW - Phenotype
KW - Pseudomonas Infections
KW - Pseudomonas aeruginosa
KW - Selection, Genetic
KW - beta-Lactams
U2 - 10.1111/j.1574-695X.2012.00983.x
DO - 10.1111/j.1574-695X.2012.00983.x
M3 - Journal article
C2 - 22540844
VL - 65
SP - 215
EP - 225
JO - Pathogens and Disease
JF - Pathogens and Disease
SN - 2049-632X
IS - 2
ER -
ID: 49278578