Pseudomonas aeruginosa mutations in lasI and rhlI quorum sensing systems result in milder chronic lung infection
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Pseudomonas aeruginosa mutations in lasI and rhlI quorum sensing systems result in milder chronic lung infection. / Wu, H; Song, Z; Givskov, Michael; Doring, G; Worlitzsch, D; Mathee, K; Rygaard, J; Høiby, N.
In: Microbiology, Vol. 147, No. Pt 5, 2001, p. 1105-1113.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Pseudomonas aeruginosa mutations in lasI and rhlI quorum sensing systems result in milder chronic lung infection
AU - Wu, H
AU - Song, Z
AU - Givskov, Michael
AU - Doring, G
AU - Worlitzsch, D
AU - Mathee, K
AU - Rygaard, J
AU - Høiby, N
PY - 2001
Y1 - 2001
N2 - To understand the importance of quorum sensing in chronic Pseudomonas aeruginosa lung infection, the in vivo pathogenic effects of the wild-type P. aeruginosa PAO1 and its double mutant, PAO1 lasI rhlI, in which the signal-generating parts of the quorum sensing systems are defective were compared. The rat model of P. aeruginosa lung infection was used in the present study. The rats were killed on days 3, 7, 14 and 28 after infection with the P. aeruginosa strains. The results showed that during the early stages of infection, the PAO1 double mutant induced a stronger serum antibody response, higher production of pulmonary interferon gamma, and more powerful blood polymorphonuclear leukocyte (PMN) chemiluminescence compared to its wild-type counterpart. On days 14 and 28 post-infection, significantly milder lung pathology, a reduction in the number of mast cells present in the lung foci, a reduced number of lung bacteria, and minor serum IgG and IgG1 responses but increased lung interferon gamma production were detected in the group infected with the PAO1 double mutant when compared with the PAO1-infected group. Delayed immune responses were observed in the PAO1-infected group and they might be associated with the production of virulence factors that are controlled by the quorum sensing systems. The conclusion of this study is that functional lasI and rhlI genes of P. aeruginosa PAO1 play a significant role during lung infection.
AB - To understand the importance of quorum sensing in chronic Pseudomonas aeruginosa lung infection, the in vivo pathogenic effects of the wild-type P. aeruginosa PAO1 and its double mutant, PAO1 lasI rhlI, in which the signal-generating parts of the quorum sensing systems are defective were compared. The rat model of P. aeruginosa lung infection was used in the present study. The rats were killed on days 3, 7, 14 and 28 after infection with the P. aeruginosa strains. The results showed that during the early stages of infection, the PAO1 double mutant induced a stronger serum antibody response, higher production of pulmonary interferon gamma, and more powerful blood polymorphonuclear leukocyte (PMN) chemiluminescence compared to its wild-type counterpart. On days 14 and 28 post-infection, significantly milder lung pathology, a reduction in the number of mast cells present in the lung foci, a reduced number of lung bacteria, and minor serum IgG and IgG1 responses but increased lung interferon gamma production were detected in the group infected with the PAO1 double mutant when compared with the PAO1-infected group. Delayed immune responses were observed in the PAO1-infected group and they might be associated with the production of virulence factors that are controlled by the quorum sensing systems. The conclusion of this study is that functional lasI and rhlI genes of P. aeruginosa PAO1 play a significant role during lung infection.
KW - Animals
KW - Bacterial Proteins
KW - Chronic Disease
KW - Cytokines
KW - Enzyme-Linked Immunosorbent Assay
KW - Female
KW - Ligases
KW - Luminescent Measurements
KW - Lung
KW - Lung Diseases
KW - Mutation
KW - Pseudomonas Infections
KW - Pseudomonas aeruginosa
KW - Rats
KW - Rats, Inbred Lew
KW - Transcription Factors
KW - Virulence
M3 - Journal article
C2 - 11320114
VL - 147
SP - 1105
EP - 1113
JO - Microbiology
JF - Microbiology
SN - 1350-0872
IS - Pt 5
ER -
ID: 143564