Quantification of specific E. coli in gut mucosa from Crohn's disease patients
Research output: Contribution to journal › Journal article › Research › peer-review
We here present a method based on qRT-PCR to quantify E. coli LF82 in intestinal human samples. Two different primer-probe sets were designed to detect LF82, and a third to target total E. coli. The assay showed high robustness and specificity for detection of LF82 in the presence of intestinal tissue.
| Original language | English |
|---|---|
| Journal | Journal of Microbiological Methods |
| Volume | 86 |
| Issue number | 1 |
| Pages (from-to) | 111-4 |
| Number of pages | 4 |
| ISSN | 0167-7012 |
| DOIs | |
| Publication status | Published - Jul 2011 |
- Crohn Disease, DNA Primers, Escherichia coli, Humans, Intestinal Mucosa, Polymerase Chain Reaction, Evaluation Studies, Journal Article, Research Support, Non-U.S. Gov't
Research areas
ID: 166456201