Sequence analysis of the Legionella micdadei groELS operon
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Sequence analysis of the Legionella micdadei groELS operon. / Hindersson, P; Høiby, N; Bangsborg, Jette Marie.
In: F E M S Microbiology Letters, Vol. 61, No. 1, 1991, p. 31-8.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Sequence analysis of the Legionella micdadei groELS operon
AU - Hindersson, P
AU - Høiby, N
AU - Bangsborg, Jette Marie
PY - 1991
Y1 - 1991
N2 - A 2.7 kb DNA fragment encoding the 60 kDa common antigen (CA) and a 13 kDa protein of Legionella micdadei was sequenced. Two open reading frames of 57,677 and 10,456 Da were identified, corresponding to the heat shock proteins GroEL and GroES, respectively. Typical -35, -10, and Shine-Dalgarno heat shock expression signals were identified upstream of the L. micdadei groEL gene. Further upstream, a poly-T region, also a feature of the sigma 32-regulated Escherichia coli groELS heat shock operon, was found. Despite the high degree of homology of the expression signals in E. coli and L. micdadei, Western blot analysis with an L. micdadei specific anti-groEL antibody did not reveal a significant increase in the amount of the GroEL protein during heat shock in L. micdadei or in the recombinant E. coli expressing L. micdadei GroEL.
AB - A 2.7 kb DNA fragment encoding the 60 kDa common antigen (CA) and a 13 kDa protein of Legionella micdadei was sequenced. Two open reading frames of 57,677 and 10,456 Da were identified, corresponding to the heat shock proteins GroEL and GroES, respectively. Typical -35, -10, and Shine-Dalgarno heat shock expression signals were identified upstream of the L. micdadei groEL gene. Further upstream, a poly-T region, also a feature of the sigma 32-regulated Escherichia coli groELS heat shock operon, was found. Despite the high degree of homology of the expression signals in E. coli and L. micdadei, Western blot analysis with an L. micdadei specific anti-groEL antibody did not reveal a significant increase in the amount of the GroEL protein during heat shock in L. micdadei or in the recombinant E. coli expressing L. micdadei GroEL.
KW - Amino Acid Sequence
KW - Bacterial Proteins
KW - Base Sequence
KW - Chaperonin 60
KW - DNA, Bacterial
KW - Escherichia coli
KW - Gene Expression Regulation, Bacterial
KW - Heat-Shock Proteins
KW - Legionella
KW - Molecular Sequence Data
KW - Molecular Weight
KW - Open Reading Frames
KW - Operon
KW - Regulatory Sequences, Nucleic Acid
KW - Restriction Mapping
KW - Sequence Homology, Nucleic Acid
M3 - Journal article
C2 - 1672279
VL - 61
SP - 31
EP - 38
JO - F E M S Microbiology Letters
JF - F E M S Microbiology Letters
SN - 0378-1097
IS - 1
ER -
ID: 40334306