Serological diagnosis of experimental Enterococcus faecalis endocarditis
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Serological diagnosis of experimental Enterococcus faecalis endocarditis. / Kjerulf, A; Espersen, F; Gutschik, E; Majcherczyk, P A; Hougen, H P; Rygaard, J; Høiby, N.
In: A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica, Vol. 106, No. 10, 1998, p. 997-1008.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Serological diagnosis of experimental Enterococcus faecalis endocarditis
AU - Kjerulf, A
AU - Espersen, F
AU - Gutschik, E
AU - Majcherczyk, P A
AU - Hougen, H P
AU - Rygaard, J
AU - Høiby, N
PY - 1998
Y1 - 1998
N2 - A modified rat model of endocarditis with catheterization for 2 days was established in female Lewis rats using different inocula of Enterococcus faecalis (strain no. EF 19) in order to measure IgG antibodies in serum during the course of infection. Increasing the inocula intravenously resulted in an increase in the CFU/g vegetation and the CFU/g spleen, the ID50 being about 10 CFU/ml and the ID90 about 1x10(2) CFU/ml. The lowest bacterial inoculum infecting 100% of the rats was 3x10(3) CFU/ml, and for further investigations we used this inoculum size. Rats were sacrificed on day 2, 5, 7, 9, 11 and 28 after infection. The CFU/g vegetation and the CFU/g spleen increased until day 7 and then decreased. Serum samples were collected from 129 rats at different times after challenge. Three different ELISA systems were established to measure the IgG antibody responses: E. faecalis sonicate ELISA (a pool of four sonicates of strain no. EF 10, EF 11, EF 19 and EF 48), E. faecalis whole cell ELISA (strain no. EF 19) and E. faecalis purified cell wall ELISA (strain no. EF 19). An IgG antibody response was detected already on day 2, and except for a minor decrease on day 6/7 the antibody response continued to increase until day 14 (whole cell ELISA and sonicate ELISA) and day 21 (purified cell wall ELISA) when a plateau was reached. Significant increases in IgG antibody responses (p
AB - A modified rat model of endocarditis with catheterization for 2 days was established in female Lewis rats using different inocula of Enterococcus faecalis (strain no. EF 19) in order to measure IgG antibodies in serum during the course of infection. Increasing the inocula intravenously resulted in an increase in the CFU/g vegetation and the CFU/g spleen, the ID50 being about 10 CFU/ml and the ID90 about 1x10(2) CFU/ml. The lowest bacterial inoculum infecting 100% of the rats was 3x10(3) CFU/ml, and for further investigations we used this inoculum size. Rats were sacrificed on day 2, 5, 7, 9, 11 and 28 after infection. The CFU/g vegetation and the CFU/g spleen increased until day 7 and then decreased. Serum samples were collected from 129 rats at different times after challenge. Three different ELISA systems were established to measure the IgG antibody responses: E. faecalis sonicate ELISA (a pool of four sonicates of strain no. EF 10, EF 11, EF 19 and EF 48), E. faecalis whole cell ELISA (strain no. EF 19) and E. faecalis purified cell wall ELISA (strain no. EF 19). An IgG antibody response was detected already on day 2, and except for a minor decrease on day 6/7 the antibody response continued to increase until day 14 (whole cell ELISA and sonicate ELISA) and day 21 (purified cell wall ELISA) when a plateau was reached. Significant increases in IgG antibody responses (p
KW - Animals
KW - Blotting, Western
KW - Cell Fractionation
KW - Cell Wall
KW - Cross Reactions
KW - Disease Models, Animal
KW - Electrophoresis, Polyacrylamide Gel
KW - Endocarditis, Bacterial
KW - Enterococcus faecalis
KW - Enzyme-Linked Immunosorbent Assay
KW - Female
KW - Gram-Positive Bacterial Infections
KW - Heart Valves
KW - Rats
KW - Rats, Inbred Lew
M3 - Journal article
C2 - 9833704
VL - 106
SP - 997
EP - 1008
JO - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica
JF - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica
SN - 0903-4641
IS - 10
ER -
ID: 44353941