Synergy of Quorum Quenching Enzyme and Quorum Sensing Inhibitor in Inhibiting P.aeruginosa Quorum Sensing

Research output: Working paper › Preprint › Research

Standard

Synergy of Quorum Quenching Enzyme and Quorum Sensing Inhibitor in Inhibiting P.aeruginosa Quorum Sensing. / Fong, J.; Zhang, C.; Yang, R.; Boo, Z.Z.; Tan, S.K.; Nielsen, T.E.; Givskov, M.; Wu, B.; Su, H.; Yang, L.

bioRxiv, 2017.

Research output: Working paper › Preprint › Research

Harvard

Fong, J, Zhang, C, Yang, R, Boo, ZZ, Tan, SK, Nielsen, TE, Givskov, M, Wu, B, Su, H & Yang, L 2017 'Synergy of Quorum Quenching Enzyme and Quorum Sensing Inhibitor in Inhibiting P.aeruginosa Quorum Sensing' bioRxiv. https://doi.org/10.1101/182543

APA

Fong, J., Zhang, C., Yang, R., Boo, Z. Z., Tan, S. K., Nielsen, T. E., Givskov, M., Wu, B., Su, H., & Yang, L. (2017). Synergy of Quorum Quenching Enzyme and Quorum Sensing Inhibitor in Inhibiting P.aeruginosa Quorum Sensing. bioRxiv. https://doi.org/10.1101/182543

Vancouver

Fong J, Zhang C, Yang R, Boo ZZ, Tan SK, Nielsen TE et al. Synergy of Quorum Quenching Enzyme and Quorum Sensing Inhibitor in Inhibiting P.aeruginosa Quorum Sensing. bioRxiv. 2017. https://doi.org/10.1101/182543

Author

Fong, J. ; Zhang, C. ; Yang, R. ; Boo, Z.Z. ; Tan, S.K. ; Nielsen, T.E. ; Givskov, M. ; Wu, B. ; Su, H. ; Yang, L. / Synergy of Quorum Quenching Enzyme and Quorum Sensing Inhibitor in Inhibiting P.aeruginosa Quorum Sensing. bioRxiv, 2017.

Bibtex

@techreport{e9191c3f4f924704b6b1f561c4534268,
title = "Synergy of Quorum Quenching Enzyme and Quorum Sensing Inhibitor in Inhibiting P.aeruginosa Quorum Sensing",
abstract = "The threat of antibiotic resistant bacteria has called for alternative antimicrobial strategies that would mitigate the increase of classical resistance mechanism. Many bacteria employ quorum sensing (QS) to govern the production of virulence genes and formation of drug-resistance biofilms. Blocking QS mechanisms have proven to be a functional alternative to conventional antibiotic control of infections. The concepts of quorum sensing inhibitors (QSI) and quorum quenching enzymes (QQ) have been investigated separately. In this study however, we simulated the synergistic effect of QQ and QSI in blocking bacterial QS. This effect was validated by experiments using AiiA and G1 as QQ and QSI respectively on Pseudomonas aeruginosa LasR/I and RhlR/I QS circuits. The combination of a QQ and a QSI almost completely blocked the P. aeruginosa QS las and rhl system. Our findings provided a potential application strategy for bacterial QS disruption.",
author = "J. Fong and C. Zhang and R. Yang and Z.Z. Boo and S.K. Tan and T.E. Nielsen and M. Givskov and B. Wu and H. Su and L. Yang",
year = "2017",
doi = "10.1101/182543",
language = "Udefineret/Ukendt",
publisher = "bioRxiv",
type = "WorkingPaper",
institution = "bioRxiv",

}

RIS

TY - UNPB

T1 - Synergy of Quorum Quenching Enzyme and Quorum Sensing Inhibitor in Inhibiting P.aeruginosa Quorum Sensing

AU - Fong, J.

AU - Zhang, C.

AU - Yang, R.

AU - Boo, Z.Z.

AU - Tan, S.K.

AU - Nielsen, T.E.

AU - Givskov, M.

AU - Wu, B.

AU - Su, H.

AU - Yang, L.

PY - 2017

Y1 - 2017

N2 - The threat of antibiotic resistant bacteria has called for alternative antimicrobial strategies that would mitigate the increase of classical resistance mechanism. Many bacteria employ quorum sensing (QS) to govern the production of virulence genes and formation of drug-resistance biofilms. Blocking QS mechanisms have proven to be a functional alternative to conventional antibiotic control of infections. The concepts of quorum sensing inhibitors (QSI) and quorum quenching enzymes (QQ) have been investigated separately. In this study however, we simulated the synergistic effect of QQ and QSI in blocking bacterial QS. This effect was validated by experiments using AiiA and G1 as QQ and QSI respectively on Pseudomonas aeruginosa LasR/I and RhlR/I QS circuits. The combination of a QQ and a QSI almost completely blocked the P. aeruginosa QS las and rhl system. Our findings provided a potential application strategy for bacterial QS disruption.

AB - The threat of antibiotic resistant bacteria has called for alternative antimicrobial strategies that would mitigate the increase of classical resistance mechanism. Many bacteria employ quorum sensing (QS) to govern the production of virulence genes and formation of drug-resistance biofilms. Blocking QS mechanisms have proven to be a functional alternative to conventional antibiotic control of infections. The concepts of quorum sensing inhibitors (QSI) and quorum quenching enzymes (QQ) have been investigated separately. In this study however, we simulated the synergistic effect of QQ and QSI in blocking bacterial QS. This effect was validated by experiments using AiiA and G1 as QQ and QSI respectively on Pseudomonas aeruginosa LasR/I and RhlR/I QS circuits. The combination of a QQ and a QSI almost completely blocked the P. aeruginosa QS las and rhl system. Our findings provided a potential application strategy for bacterial QS disruption.

U2 - 10.1101/182543

DO - 10.1101/182543

M3 - Preprint

BT - Synergy of Quorum Quenching Enzyme and Quorum Sensing Inhibitor in Inhibiting P.aeruginosa Quorum Sensing

PB - bioRxiv

ER -

ID: 340401178