The Bep gene cluster in Burkholderia cenocepacia H111 codes for a water-insoluble exopolysaccharide essential for biofilm formation

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

The Bep gene cluster in Burkholderia cenocepacia H111 codes for a water-insoluble exopolysaccharide essential for biofilm formation. / Bellich, Barbara; Terán, Lucrecia C.; Fazli, Magnus M.; Berti, Francesco; Rizzo, Roberto; Tolker-Nielsen, Tim; Cescutti, Paola.

In: Carbohydrate Polymers, Vol. 301, 120318, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Bellich, B, Terán, LC, Fazli, MM, Berti, F, Rizzo, R, Tolker-Nielsen, T & Cescutti, P 2023, 'The Bep gene cluster in Burkholderia cenocepacia H111 codes for a water-insoluble exopolysaccharide essential for biofilm formation', Carbohydrate Polymers, vol. 301, 120318. https://doi.org/10.1016/j.carbpol.2022.120318

APA

Bellich, B., Terán, L. C., Fazli, M. M., Berti, F., Rizzo, R., Tolker-Nielsen, T., & Cescutti, P. (2023). The Bep gene cluster in Burkholderia cenocepacia H111 codes for a water-insoluble exopolysaccharide essential for biofilm formation. Carbohydrate Polymers, 301, [120318]. https://doi.org/10.1016/j.carbpol.2022.120318

Vancouver

Bellich B, Terán LC, Fazli MM, Berti F, Rizzo R, Tolker-Nielsen T et al. The Bep gene cluster in Burkholderia cenocepacia H111 codes for a water-insoluble exopolysaccharide essential for biofilm formation. Carbohydrate Polymers. 2023;301. 120318. https://doi.org/10.1016/j.carbpol.2022.120318

Author

Bellich, Barbara ; Terán, Lucrecia C. ; Fazli, Magnus M. ; Berti, Francesco ; Rizzo, Roberto ; Tolker-Nielsen, Tim ; Cescutti, Paola. / The Bep gene cluster in Burkholderia cenocepacia H111 codes for a water-insoluble exopolysaccharide essential for biofilm formation. In: Carbohydrate Polymers. 2023 ; Vol. 301.

Bibtex

@article{57d45d6ad26b499eab163fe7356ca558,
title = "The Bep gene cluster in Burkholderia cenocepacia H111 codes for a water-insoluble exopolysaccharide essential for biofilm formation",
abstract = "Burkholderia cenocepacia is an opportunistic pathogen isolated from cystic fibrosis patients where it causes infections that are extremely difficult to treat with antibiotics, and sometimes have a fatal outcome. Biofilm is a virulence trait of B. cenocepacia, and is associated with infection persistence and increased tolerance to antibiotics. In biofilms exopolysaccharides have an important role, conferring mechanical stability and antibiotic tolerance. Two different exopolysaccharides were isolated from B. cenocepacia H111 biofilms: a water-soluble polysaccharide rich in rhamnose and containing an L-Man residue, and a water-insoluble polymer made of glucose, galactose and mannose. In the present work, the product encoded by B. cenocepacia H111 bepA-L gene cluster was identified as the water-insoluble exopolysaccharide, using mutant strains and NMR spectroscopy of the purified polysaccharides. It was also demonstrated that the B. cenocepacia H111 wild type strain produces the water-insoluble exopolysaccharide in pellicles, thus underlining its potential importance in in vivo infections.",
keywords = "bepA-L gene cluster, Biofilm, Burkholderia cenocepacia, Exopolysaccharide structure, NMR spectroscopy",
author = "Barbara Bellich and Ter{\'a}n, {Lucrecia C.} and Fazli, {Magnus M.} and Francesco Berti and Roberto Rizzo and Tim Tolker-Nielsen and Paola Cescutti",
note = "Publisher Copyright: {\textcopyright} 2022 Elsevier Ltd",
year = "2023",
doi = "10.1016/j.carbpol.2022.120318",
language = "English",
volume = "301",
journal = "Carbohydrate Polymers",
issn = "0144-8617",
publisher = "Pergamon Press",

}

RIS

TY - JOUR

T1 - The Bep gene cluster in Burkholderia cenocepacia H111 codes for a water-insoluble exopolysaccharide essential for biofilm formation

AU - Bellich, Barbara

AU - Terán, Lucrecia C.

AU - Fazli, Magnus M.

AU - Berti, Francesco

AU - Rizzo, Roberto

AU - Tolker-Nielsen, Tim

AU - Cescutti, Paola

N1 - Publisher Copyright: © 2022 Elsevier Ltd

PY - 2023

Y1 - 2023

N2 - Burkholderia cenocepacia is an opportunistic pathogen isolated from cystic fibrosis patients where it causes infections that are extremely difficult to treat with antibiotics, and sometimes have a fatal outcome. Biofilm is a virulence trait of B. cenocepacia, and is associated with infection persistence and increased tolerance to antibiotics. In biofilms exopolysaccharides have an important role, conferring mechanical stability and antibiotic tolerance. Two different exopolysaccharides were isolated from B. cenocepacia H111 biofilms: a water-soluble polysaccharide rich in rhamnose and containing an L-Man residue, and a water-insoluble polymer made of glucose, galactose and mannose. In the present work, the product encoded by B. cenocepacia H111 bepA-L gene cluster was identified as the water-insoluble exopolysaccharide, using mutant strains and NMR spectroscopy of the purified polysaccharides. It was also demonstrated that the B. cenocepacia H111 wild type strain produces the water-insoluble exopolysaccharide in pellicles, thus underlining its potential importance in in vivo infections.

AB - Burkholderia cenocepacia is an opportunistic pathogen isolated from cystic fibrosis patients where it causes infections that are extremely difficult to treat with antibiotics, and sometimes have a fatal outcome. Biofilm is a virulence trait of B. cenocepacia, and is associated with infection persistence and increased tolerance to antibiotics. In biofilms exopolysaccharides have an important role, conferring mechanical stability and antibiotic tolerance. Two different exopolysaccharides were isolated from B. cenocepacia H111 biofilms: a water-soluble polysaccharide rich in rhamnose and containing an L-Man residue, and a water-insoluble polymer made of glucose, galactose and mannose. In the present work, the product encoded by B. cenocepacia H111 bepA-L gene cluster was identified as the water-insoluble exopolysaccharide, using mutant strains and NMR spectroscopy of the purified polysaccharides. It was also demonstrated that the B. cenocepacia H111 wild type strain produces the water-insoluble exopolysaccharide in pellicles, thus underlining its potential importance in in vivo infections.

KW - bepA-L gene cluster

KW - Biofilm

KW - Burkholderia cenocepacia

KW - Exopolysaccharide structure

KW - NMR spectroscopy

U2 - 10.1016/j.carbpol.2022.120318

DO - 10.1016/j.carbpol.2022.120318

M3 - Journal article

C2 - 36436859

AN - SCOPUS:85141911943

VL - 301

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

SN - 0144-8617

M1 - 120318

ER -

ID: 330895722