TY - JOUR

T1 - The biofilm of Burkholderia cenocepacia H111 contains an exopolysaccharide composed of L-rhamnose and L-mannose

T2 - Structural characterization and molecular modelling

AU - Bellich, Barbara

AU - Jou, Ining A.

AU - Buriola, Claudia

AU - Ravenscroft, Neil

AU - Brady, John W.

AU - Fazli, Mustafa

AU - Tolker-Nielsen, Tim

AU - Rizzo, Roberto

AU - Cescutti, Paola

PY - 2021

Y1 - 2021

N2 - Burkholderia cenocepacia belongs to the Burkholderia Cepacia Complex, a group of 22 closely related species both of clinical and environmental origin, infecting cystic fibrosis patients. B. cenocepacia accounts for the majority of the clinical isolates, comprising the most virulent and transmissible strains. The capacity to form biofilms is among the many virulence determinants of B. cenocepacia, a characteristic that confers enhanced tolerance to some antibiotics, desiccation, oxidizing agents, and host defenses. Exopolysaccharides are a major component of biofilm matrices, particularly providing mechanical stability to biofilms. Recently, a water-insoluble exopolysaccharide produced by B. cenocepacia H111 in biofilm was characterized. In the present study, a water-soluble exopolysaccharide was extracted from B. cenocepacia H111 biofilm, and its structure was determined by GLC-MS, NMR and ESI-MS. The repeating unit is a linear rhamno-tetrasaccharide with 50% replacement of a 3-α-L-Rha with a α-3-L-Man. [2)-α-L-Rhap-(1→3)-α-L-[Rhap or Manp]-(1→3)-α-L-Rhap-(1→2)-α-L-Rhap-(1→]n Molecular modelling was used to obtain information about local structural motifs which could give information about the polysaccharide conformation.

AB - Burkholderia cenocepacia belongs to the Burkholderia Cepacia Complex, a group of 22 closely related species both of clinical and environmental origin, infecting cystic fibrosis patients. B. cenocepacia accounts for the majority of the clinical isolates, comprising the most virulent and transmissible strains. The capacity to form biofilms is among the many virulence determinants of B. cenocepacia, a characteristic that confers enhanced tolerance to some antibiotics, desiccation, oxidizing agents, and host defenses. Exopolysaccharides are a major component of biofilm matrices, particularly providing mechanical stability to biofilms. Recently, a water-insoluble exopolysaccharide produced by B. cenocepacia H111 in biofilm was characterized. In the present study, a water-soluble exopolysaccharide was extracted from B. cenocepacia H111 biofilm, and its structure was determined by GLC-MS, NMR and ESI-MS. The repeating unit is a linear rhamno-tetrasaccharide with 50% replacement of a 3-α-L-Rha with a α-3-L-Man. [2)-α-L-Rhap-(1→3)-α-L-[Rhap or Manp]-(1→3)-α-L-Rhap-(1→2)-α-L-Rhap-(1→]n Molecular modelling was used to obtain information about local structural motifs which could give information about the polysaccharide conformation.

KW - Biofilm

KW - Burkholderia cenocepacia H111

KW - Click here for more information

KW - For information on how to create MOL files please see here

KW - L-mannose

KW - Molecular modelling

KW - NMR

KW - OPTIONAL

KW - Polysaccharide structure

KW - You can provide MOL files of the most relevant compounds discussed in your paper when you return the corrections

U2 - 10.1016/j.carres.2020.108231

DO - 10.1016/j.carres.2020.108231

M3 - Journal article

C2 - 33440288

AN - SCOPUS:85099254113

VL - 499

JO - Carbohydrate Research

JF - Carbohydrate Research

SN - 0008-6215

M1 - 108231

ER -