The divergent promoters mediating transcription of the par locus of plasmid RP4 are subject to autoregulation
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The divergent promoters mediating transcription of the par locus of plasmid RP4 are subject to autoregulation. / Eberl, Leo; Givskov, M; Schwab, H.
In: Molecular Microbiology, Vol. 6, No. 14, 1992, p. 1969-79.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - The divergent promoters mediating transcription of the par locus of plasmid RP4 are subject to autoregulation
AU - Eberl, Leo
AU - Givskov, M
AU - Schwab, H
PY - 1992
Y1 - 1992
N2 - The partitioning region of broad-host-range plasmid RP4 contains four genes (parA, parB, parC, and parD) that encode products essential for partition activity. Two divergently arranged promoters located in the intercistronic region between parC and parD mediate transcription of these genes. The transcriptional initiation sites for both promoters were determined by primer extension. Transcriptional fusions were used to show that parA, parB, and parC are combined in an operon, while parD constitutes a separate transcription unit. Both parCBA (genes in order of transcription) and parD are negatively autoregulated at the level of transcription by the gene products of parA and parD, respectively. parD promoter mutants which have become insensitive to repression by parD were isolated. Comparison of wild type and the mutant parD promoter sequences indicated that three short repeats are likely involved in the negative regulation of this promoter. Potentially these sequence elements comprise target sites for the ParD protein.
AB - The partitioning region of broad-host-range plasmid RP4 contains four genes (parA, parB, parC, and parD) that encode products essential for partition activity. Two divergently arranged promoters located in the intercistronic region between parC and parD mediate transcription of these genes. The transcriptional initiation sites for both promoters were determined by primer extension. Transcriptional fusions were used to show that parA, parB, and parC are combined in an operon, while parD constitutes a separate transcription unit. Both parCBA (genes in order of transcription) and parD are negatively autoregulated at the level of transcription by the gene products of parA and parD, respectively. parD promoter mutants which have become insensitive to repression by parD were isolated. Comparison of wild type and the mutant parD promoter sequences indicated that three short repeats are likely involved in the negative regulation of this promoter. Potentially these sequence elements comprise target sites for the ParD protein.
M3 - Journal article
C2 - 1508044
VL - 6
SP - 1969
EP - 1979
JO - Molecular Microbiology
JF - Molecular Microbiology
SN - 0950-382X
IS - 14
ER -
ID: 44293698